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A preparation method of silk fibroin/hyaluronic acid double network hydrogel that can realize three-dimensional loading of cells

A technology of silk fibroin and hyaluronic acid, applied in prosthesis, medical science and other directions, can solve the problems of inability to meet the requirements of three-dimensional cartilage construction, three-dimensional loading of difficult cells, and long gelation time, and achieve adhesion, proliferation and In-situ three-dimensional loading, green and environmentally friendly gel forming method, and the effect of stable physical and chemical properties

Active Publication Date: 2022-04-19
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the current methods for preparing double network hydrogels use toxic cross-linking agents, or the gelation time is too long, it is difficult to achieve three-dimensional loading of cells in vitro, and cannot meet the requirements of three-dimensional construction of tissues such as cartilage in vitro

Method used

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  • A preparation method of silk fibroin/hyaluronic acid double network hydrogel that can realize three-dimensional loading of cells

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Embodiment 1

[0034] 1. A method for preparing a silk fibroin / hyaluronic acid double network hydrogel capable of three-dimensional loading of cells, comprising the steps of:

[0035] 1) Sterilization of materials: The silk fibroin solution and the modified hyaluronic acid solution were sterilized separately under the high temperature and high pressure environment of 120° C. and 0.25 MPa, and the sterilization time was 20 minutes.

[0036] 2) Mix the silk fibroin solution with a mass volume fraction of 8% and the modified hyaluronic acid solution with a mass volume fraction of 2% according to a mass ratio of 10:0 to form a prepolymer solution, and add photoinitiated Agent 2-hydroxyl-4'-(2-hydroxyethoxy)-2-methyl propiophenone, wherein the mass volume ratio (g / ml) of photoinitiator and prepolymer solution is 0.5%;

[0037] 3) The solution obtained in step 2) was sonicated for 15 s, and the solution was fluid and transparent after sonication;

[0038] 4) After the solution obtained in step 3)...

Embodiment 2

[0040] 1. A method for preparing a silk fibroin / hyaluronic acid double network hydrogel capable of three-dimensional loading of cells, comprising the steps of:

[0041] 1) Sterilization of materials: adopt the method of high temperature and high pressure in advance, and sterilize the silk fibroin solution and the modified hyaluronic acid solution for 20 minutes at 120°C and 0.25Mpa high temperature and high pressure environment;

[0042] 2) The silk fibroin solution with a mass volume fraction of 8%, and the methacrylic anhydride-modified hyaluronic acid solution with a mass volume fraction of 2% are mixed according to a mass ratio of 9.5:0.5 to form a prepolymer solution, and the prepolymer solution is Add photoinitiator 2-hydroxyl-4'-(2-hydroxyethoxy)-2-methyl propiophenone, wherein, the mass volume ratio (g / ml) of photoinitiator and prepolymer solution is 0.5% ;

[0043]3) The solution obtained in step 2) was sonicated for 13s, and the solution was fluid and transparent af...

Embodiment 3

[0046] 1. A method for preparing a silk fibroin / hyaluronic acid double network hydrogel capable of encapsulating cells, comprising the steps of:

[0047] 1) Sterilization of materials: The silk fibroin solution and the modified hyaluronic acid solution were sterilized separately under the high temperature and high pressure environment of 120° C. and 0.25 MPa, and the sterilization time was 20 minutes.

[0048] 2) Mix the silk fibroin solution with a mass volume fraction of 8% and the modified hyaluronic acid solution with a mass volume fraction of 2% according to a mass ratio of 9:1 to form a prepolymer solution, and add photoinitiated Agent 2-hydroxyl-4'-(2-hydroxyethoxy)-2-methylpropiophenone, the mass volume ratio (g / ml) of photoinitiator and prepolymer solution is 0.5%;

[0049] 3) Ultrasonicate the solution obtained in step 2) for 12s, and the solution is fluid and transparent after ultrasonication;

[0050] 4) After the solution obtained in step 3) was repeatedly blown ...

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Abstract

The invention discloses a preparation method of a silk fibroin / hyaluronic acid double network hydrogel capable of realizing three-dimensional loading of cells, which comprises the following steps: 1) sterilizing the material: 2) making the silk fibroin solution, modified transparent The urethane solution is mixed to form a prepolymer solution, and a photoinitiator is added to the prepolymer solution; 3) the prepolymer solution obtained in the ultrasonic treatment step 2), the solution has fluidity and is translucent after ultrasonic treatment; 4) Step 3) processing the obtained solution and mixing the cells evenly to obtain a cell pre-aggregation solution, and further adding the cell pre-aggregation solution to the cell culture plate; 5) placing the culture plate obtained in step 4) under ultraviolet light for photocrosslinking into a gel, and then Place it in a thermostat at 37°C until the pre-polymerization solution turns milky white. The method can realize the three-dimensional culture of cells in the high-strength and high-toughness double network hydrogel, which is beneficial to the three-dimensional construction of load-bearing soft tissue tissues such as cartilage in vitro.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomaterials, and in particular relates to a preparation method of a silk fibroin / hyaluronic acid double network hydrogel capable of realizing three-dimensional loading of cells. Background technique [0002] The hydrogel material is a kind of polymer with high water absorption and water retention, soft texture and three-dimensional network system, which is similar to the texture of living soft tissue; because its three-dimensional network structure is similar to natural extracellular matrix, it is conducive to the growth of seed cells. Survival; it can be minimally invasively injected into the body in a liquid state and then form a semi-solid colloid to reduce implant trauma and other unique advantages. It has been widely used in the research of scaffold materials in tissue engineering. However, hydrogels have disadvantages such as poor mechanical properties and rapid degradation, which make the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24C08L89/00C08L5/08A61L27/20A61L27/22A61L27/52
Inventor 肖文谦曲晓航李加乐谭云飞李波
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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