Test strip for testing chlorpromazine by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

A time-resolved fluorescence and immunochromatography technology, applied in measurement devices, analytical materials, instruments, etc., can solve problems such as low sensitivity, large batch-to-batch variation, and small stock displacement, and achieve the effect of high sensitivity

Inactive Publication Date: 2019-05-28
ZHENJIANG YITE BIOTECH DEV CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Test strip for testing chlorpromazine by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing
  • Test strip for testing chlorpromazine by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing
  • Test strip for testing chlorpromazine by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

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Experimental program
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Embodiment Construction

[0034] 1. Preparation of Components of Test Strips

[0035] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotinylated γ-globulin (BGG)

[0036] ⑴ Preparation of biotinylated BGG

[0037] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.2 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μl Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M Nacl, 0.005 MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0038] ⑵ Use the above-mentioned labeled protein to coat the aldehyde-modified fluorescent microspheres

[0...

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Abstract

The invention relates to a preparation method and application of a quick test strip for testing chlorpromazine by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing. Thetest strip is composed of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper, according to the competition law principle, time-resolved fluorescence microspheres are taken as animmune marker, and accurate and quick quantitative testing can be conducted on a to-be-tested antigen.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of chlorpromazine and a preparation method and application thereof. Background technique [0002] Chlorpromazine belongs to phenothiazine metabolites. This drug is mainly metabolized in the liver, and it is easy to produce drug residues. Residual chlorpromazine and some active metabolites of the original drug can cause leukopenia and agranulocytosis, resulting in Human liver and kidney lesions can also cause eye complications. Breeders in some countries and regions add chlorpromazine to food animals, resulting in chlorpromazine residues in related animal foods. Therefore, regular detection of chlorpromazine residues has become a necessary means of monitoring in many countries . [0003] Current immunochromatogr...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558
Inventor 杜霞袁超丁炎
Owner ZHENJIANG YITE BIOTECH DEV CO LTD
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