Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of culture medium for enhancing acid resistance of bacillus

The technology of bacillus and culture medium is applied in the field of preparation of culture medium, which can solve the problem of low bacterial concentration and activity, achieve high bacterial concentration and activity, increase acid resistance and improve application effect.

Active Publication Date: 2019-05-31
共 放鸣
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bacillus cultured by traditional techniques, the concentration and activity of the bacteria are not high in acidic environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In order to highlight the role of this medium in promoting the acid resistance of unacclimated bacillus, this example conducts a comparative acid-resistant culture test on unacclimated bacillus.

[0020] A group of test groups and a group of control groups are set, the culture fluid used in the test group is the culture fluid described in this patent, and the culture fluid of the control group is the common culture fluid that does not use graphite silicon.

[0021] The preparation method of the culture medium used in the test group is as follows:

[0022] 1) Crush the graphite silicon and screen the particles with a diameter of 3mm.

[0023] 2) To make the culture medium, add 2.0g glucose, 1.5g peptone, 0.5g sodium chloride, 0.05g beef extract and 3g graphite silicon particles per 100g water, mix well, adjust the pH to 5.0, and press at 121°C under high pressure Sterilize for 20 minutes. By using the high temperature during sterilization, the role of graphite silicon ...

Embodiment 2

[0033] The acid resistance test was carried out on the domesticated bacillus.

[0034] The culture medium of the invention is used to carry out continuous acid-resistant acclimation to the bacillus, and the acid-resistant bacillus is obtained by gradually reducing the pH value of the medium.

[0035] The specific process of continuous acid-resistant acclimatization: gradually reduce the pH value of the medium, set the pH value of the medium at each stage from high to low: 5.5, 5.0, 4.5, 4.0, 3.7, 3.5, 3.3, 3.2, each pH value passage 3- 5 times, repeated acclimatization and then inoculated to the next pH value, and selected acid-resistant Bacillus colonies after each acclimation, and finally obtained the strain with the strongest growth ability in the acidic environment.

[0036] Keep the culture temperature at 36~38℃ during the continuous acclimatization process. This temperature is not only suitable for the cultivation of Bacillus, but also the graphite silicon component in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a culture medium for enhancing acid resistance of bacillus. The preparation method comprises the steps of crushing graphite silicon to prepare the culture medium, adding graphite silicon particles and nutrient substances into water, fully mixing, adjusting pH, and sterilizing the mixture under high temperature and high pressure; and adding the bacillus into the culture solution containing the graphite silicon particles for culture. According to the method, by improving a processing technology of the bacillus, the strain with acid resistance is cultivated, so that the bacillus can keep high strain concentration and activity in an acidic environment when passing through the digestive tract of an animal, and the bacillus can smoothly proliferatein vivo and play beneficial effects. Therefore, the acid resistance of the bacillus is enhanced, and a better application effect can be exerted when the bacillus is applied to the field of feed additives.

Description

technical field [0001] The invention relates to a method for preparing a microbial culture medium, in particular to a method for preparing a culture medium for enhancing acid resistance of bacillus. Background technique [0002] The harmless and beneficial strains and strains of Bacillus to animals and humans have the functions of promoting the development of animal intestines, regulating the balance of animal intestinal flora, enhancing animal immunity, producing various enzymes, and improving the activity of digestive enzymes, etc. It is developed as an animal feed microbial additive. Because of its unique functions, Bacillus can replace antibiotics in the production of pollution-free "green" feed, and the development and application of its related products will have very broad prospects and good economic benefits. [0003] Whether the bacillus can play a role in the animal body is closely related to whether the bacillus can proliferate in the body, the location of the pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12R1/07
Inventor 共放鸣钟国盛
Owner 共 放鸣
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com