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Method for quantitatively analyzing content of alizarin red through fluorescence quenching method

A technique for fluorescence quenching and quantitative analysis, used in fluorescence/phosphorescence, material excitation analysis, etc.

Inactive Publication Date: 2019-05-31
JILIN INST OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, methods for its quantitative analysis have not been reported so far.

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  • Method for quantitatively analyzing content of alizarin red through fluorescence quenching method

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example 1

[0011] Example 1: Prepare alizarin red standard solution with a certain concentration, and adjust the pH to 6 with 1mol / L sodium hydroxide to obtain the test sample; pipette 10 μL of the test sample and add it to the nitrogen-doped graphene quantum dot solution In this method, the content of Alizarin Red in the sample to be tested is 3.003 nmol / L, and the concentration of nitrogen-doped graphene quantum dots is the same as the concentration in the above-mentioned working curve. Fluorescence is measured with a fluorescence analyzer, and the concentration to be tested is determined according to the working curve. The content of Alizarin Red in the test sample was 3.00 nmol / L, and the detection rate was 100.1%.

example 2

[0012] Example 2: Pipette 10 µL of the sample to be tested and add it to the nitrogen-doped graphene quantum dot solution, so that the content of alizarin red in the sample to be tested is 12.46 nmol / L, and the concentration of the nitrogen-doped graphene quantum dot is the same as The concentration in the above-mentioned working curve is the same, and the fluorescence is measured with a fluorescence analyzer. According to the working curve, the content of alizarin red in the sample to be tested is determined to be 12.50 nmol / L, and the detection rate is 99.68%.

example 3

[0013] Example 3: Pipette 10 µL of the sample to be tested and add it to the nitrogen-doped graphene quantum dot solution, so that the content of alizarin red in the sample to be tested is 22.91 nmol / L, and the concentration of the nitrogen-doped graphene quantum dot is the same as The concentration in the above-mentioned working curve is the same, and the fluorescence is measured with a fluorescence analyzer. According to the working curve, the content of alizarin red in the sample to be tested is determined to be 23.00 nmol / L, and the detection rate is 99.61%.

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Abstract

The invention discloses a method for quantitatively analyzing the content of alizarin red through a fluorescence quenching method. According to the method, nitrogen-doped graphene quantum dots are adopted as a fluorescent probe; on the basis of a characteristic that the fluorescence intensity of the nitrogen-doped probe is weakened with the increase of the concentration of alizarin red, high selectivity and high sensitivity detection is performed; and the change value of the fluorescence intensity of the nitrogen-doped graphene quantum dot probe is in a great linear relation with the concentration of the alizarin red, and the correlation coefficient of the fluorescence intensity of the nitrogen-doped graphene quantum dot probe and the concentration of the alizarin red is 0.992. The methodof the invention has the advantages of simple operation, high sensitivity, good selectivity, and quick detection, and is suitable for field in situ analysis.

Description

technical field [0001] The present invention proposes to design a method for quantitative analysis of alizarin red based on fluorescence quenching of nitrogen-doped graphene quantum dots. Background technique [0002] Alizarin red (Alizarin red S) chemical name is 1.2 dihydroxyanthraquinone-3-sulfonic acid sodium salt. Alizarin red is a chemical reagent and also used as a dye. It is yellow-brown or orange-yellow powder and is easily soluble in Water, the pH of 1% aqueous solution is 2.15, and its aqueous solution is light brown. After adding hydrochloric acid, it turns yellow, and after adding sodium hydroxide, it turns blue purple. It is purple when dissolved in ammonia water; slightly soluble in alcohol, insoluble in benzene , Chloroform. Alizarin red is widely used in chemical analysis. The color of alizarin red aqueous solution changes with the pH of the solution, so it can be used as an acid-base indicator. The pH value of the first color range is 4.8 (light orange)~ ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 金丽邱兢瑶张建坡
Owner JILIN INST OF CHEM TECH
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