Microfluidic dissolved oxygen detection chip and applications thereof

Abstract

The invention discloses a microfluidic dissolved oxygen detection chip and applications thereof. The microfluidic dissolved oxygen detection chip comprises a liquid flow layer and a dissolved oxygen detection microsphere capture layer, the liquid flow layer comprises more than one culture detection unit, each culture detection unit comprises more than one liquid flow pipeline, the dissolved oxygendetection microsphere capture layer comprises more than one detection microsphere cavity, each detection microsphere cavity is distributed below the corresponding liquid flow pipeline and communicates with the liquid flow pipeline, and each detection microsphere cavity is used for capturing at least one dissolved oxygen detection microsphere which is carried by a fluid flowing through the liquidflow pipeline corresponding to the detection microsphere cavity, but is settled to the dissolved oxygen detection microsphere capture layer under the action of gravity. The chip can realize multi-channel cell culture and detect the dissolved oxygen content in a real-time localized manner in the culture process, the detected object is directly contacted with the dissolved oxygen detection microspheres, and the response time is short. Meanwhile, the chip can effectively avoid the mutual cross influence among culture units.

Description

technical field [0001] The invention relates to a microfluidic dissolved oxygen detection chip, in particular to a microfluidic chip capable of multi-channel cell culture and real-time localized dissolved oxygen detection, a preparation method and application thereof, belonging to the technical field of dissolved oxygen detection. Background technique [0002] In in vitro studies of cells or tissues, the control of culture conditions is very important. The microenvironment of cells includes complex chemical and mechanical parameters, such as parameters such as oxygen tension, temperature, pH, and osmolarity that characterize physicochemical properties, and growth factors that characterize cell-cell or cell-cell matrix interactions. These parameters provide biological or medical researchers with a series of clues and prompts to adjust the structure, function and behavior of cells to achieve biological or medical purposes. Therefore, it is of great significance to study the c...

AI Technical Summary

Problems solved by technology

Although the detection throughput of this chip is high, the number of captured oxygen-sensitive microbeads in the chamber cannot be controlled, and the influence caused by uneven distribution cannot be ruled out; at the same time, the captured microspheres are easy to slip away when the liquid flows faster, thus The experiment cannot be repeated and calibrated well, and the accuracy will be affected to a certain extent

At the same time, the optical oxygen-sensitive beads used in this chip are more complicated in the synthesis steps, the time required is longer, the amount of synthesis is less, and the efficiency is low.

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