Method for extracting small molecule peptide by taking euphausia superba meal as raw material

A technology of Antarctic krill powder and small molecular peptides, which is applied in the field of bioengineering, can solve the problems of high fluorine content restricting processing and safe utilization, and achieve the effects of improving resource utilization, increasing concentration efficiency, and increasing the amount of increase

Inactive Publication Date: 2019-06-14
WEIHAI YUWANG GRP MARINE BIOLOGICAL ENGCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Antarctic krill is rich in protein and various nutrients, and is an important high-quality biological protein pool for human beings, but high fluorine content has always been a problem restricting its processing and safe utilization

Method used

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  • Method for extracting small molecule peptide by taking euphausia superba meal as raw material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention provides a method for extracting small molecular peptides from Antarctic krill powder, which is carried out according to the following steps:

[0029] Step S1, carry out hot water extraction, take 1 kg of Antarctic krill powder in the extraction tank, then add purified water twice the volume of Antarctic krill powder, that is, add 3 kg of purified water, raise the temperature to 90 ° C, and continue the extraction for 3 hours, stirring during the process The paddle keeps stirring;

[0030] Step S2, after the hot water extraction is completed, stop heating and keep the stirring paddle to continue to work. After the extraction tank is cooled to 55°C, add 1g of neutral protease to the extraction tank in sequence and stir for 15 minutes, then add 3g of alkaline protease to shear After cutting for 3 hours, add 0.5 g flavor enzyme to cut for 2 hours. After the reaction is over, raise the temperature of the extraction tank to 90° C. and keep it for 30 minutes to ...

Embodiment 2

[0039] The invention provides a method for extracting small molecular peptides from Antarctic krill powder, which is carried out according to the following steps:

[0040] Step S1, carry out hot water extraction, take 1 kg of Antarctic krill powder in the extraction tank, then add purified water 2.5 times the volume of Antarctic krill powder, that is, add 2.5 kg of purified water, raise the temperature to 98 ° C, and continue the extraction for 3 hours. The stirring paddle keeps stirring;

[0041] Step S2, after the hot water extraction is completed, stop heating and keep the stirring paddle to continue to work. After the extraction tank is cooled to 60°C, add 1 g of neutral protease to the extraction tank in sequence and stir for 15 minutes, then add 3 g of alkaline protease to shear After cutting for 3 hours, add 0.5 g flavor enzyme to cut for 2 hours. After the reaction is over, raise the temperature of the extraction tank to 98° C. and keep it for 30 minutes to inactivate th...

Embodiment 3

[0050] The invention provides a method for extracting small molecular peptides from Antarctic krill powder, which is carried out according to the following steps:

[0051] Step S1, carry out hot water extraction, take 1 kg of Antarctic krill powder in the extraction tank, then add purified water 3 times the volume of Antarctic krill powder, that is, add 3 kg of purified water, raise the temperature to 95 ° C, and continue the extraction for 3 hours, stirring during the process The paddle keeps stirring;

[0052] Step S2, after the hot water extraction is completed, stop heating and keep the stirring paddle to continue to work. After the extraction tank is cooled to 60°C, add 1 g of neutral protease to the extraction tank in sequence and stir for 15 minutes, then add 3 g of alkaline protease to shear After cutting for 3 hours, add 0.5 g flavor enzyme to cut for 2 hours. After the reaction is over, raise the temperature of the extraction tank to 95°C and keep it for 30 minutes t...

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Abstract

The invention relates to the technical field of bioengineering, in particular to a method for extracting small molecule peptide by taking euphausia superba meal as a raw material. After hot-water extraction, enzyme hydrolysis and enzyme inactivation, an enzyme shearing solution is obtained and is subjected to calcium salt precipitation and filtering, through a composite tubular membrane, micromolecular impurities and hyphae are removed, through a nanofiltration membrane, after being subjected to vacuum concentration, the enzyme shearing solution of a certain concentration is obtained, and thenthe small molecule peptide is obtained through centrifugal spray drying; the euphausia superba meal can be comprehensively utilized, the resource utilization rate of the euphausia superba meal is increased, and the additional value of the euphausia superba meal is increased.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for extracting small molecular peptides using Antarctic krill powder as a raw material. Background technique [0002] Functional protein is a natural biopolymer synthesized by animal cells. For example, collagen exists in almost all tissues as an important component of the extracellular matrix, accounting for about 30% of the total animal protein. Collagen has a high ability to resist extension, making skin, etc. The ability of connective tissue to maintain a certain structure to support and protect cells, tissues and body functions is critical to the structure and performance of cells and tissues. Collagen peptide is a peptide mixture obtained from collagen as raw material by acid / alkali treatment, enzymatic degradation or combination of multiple methods, and its main component is a polypeptide with non-uniform molecular weight formed after collagen degradation. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 白义化王欣邢晓明申世轩冯静于洋焦玲玉李春慧孙晓楠石丹璐
Owner WEIHAI YUWANG GRP MARINE BIOLOGICAL ENGCO
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