Method for extracting small molecule peptide by taking euphausia superba meal as raw material
A technology of Antarctic krill powder and small molecular peptides, which is applied in the field of bioengineering, can solve the problems of high fluorine content restricting processing and safe utilization, and achieve the effects of improving resource utilization, increasing concentration efficiency, and increasing the amount of increase
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Embodiment 1
[0028] The invention provides a method for extracting small molecular peptides from Antarctic krill powder, which is carried out according to the following steps:
[0029] Step S1, carry out hot water extraction, take 1 kg of Antarctic krill powder in the extraction tank, then add purified water twice the volume of Antarctic krill powder, that is, add 3 kg of purified water, raise the temperature to 90 ° C, and continue the extraction for 3 hours, stirring during the process The paddle keeps stirring;
[0030] Step S2, after the hot water extraction is completed, stop heating and keep the stirring paddle to continue to work. After the extraction tank is cooled to 55°C, add 1g of neutral protease to the extraction tank in sequence and stir for 15 minutes, then add 3g of alkaline protease to shear After cutting for 3 hours, add 0.5 g flavor enzyme to cut for 2 hours. After the reaction is over, raise the temperature of the extraction tank to 90° C. and keep it for 30 minutes to ...
Embodiment 2
[0039] The invention provides a method for extracting small molecular peptides from Antarctic krill powder, which is carried out according to the following steps:
[0040] Step S1, carry out hot water extraction, take 1 kg of Antarctic krill powder in the extraction tank, then add purified water 2.5 times the volume of Antarctic krill powder, that is, add 2.5 kg of purified water, raise the temperature to 98 ° C, and continue the extraction for 3 hours. The stirring paddle keeps stirring;
[0041] Step S2, after the hot water extraction is completed, stop heating and keep the stirring paddle to continue to work. After the extraction tank is cooled to 60°C, add 1 g of neutral protease to the extraction tank in sequence and stir for 15 minutes, then add 3 g of alkaline protease to shear After cutting for 3 hours, add 0.5 g flavor enzyme to cut for 2 hours. After the reaction is over, raise the temperature of the extraction tank to 98° C. and keep it for 30 minutes to inactivate th...
Embodiment 3
[0050] The invention provides a method for extracting small molecular peptides from Antarctic krill powder, which is carried out according to the following steps:
[0051] Step S1, carry out hot water extraction, take 1 kg of Antarctic krill powder in the extraction tank, then add purified water 3 times the volume of Antarctic krill powder, that is, add 3 kg of purified water, raise the temperature to 95 ° C, and continue the extraction for 3 hours, stirring during the process The paddle keeps stirring;
[0052] Step S2, after the hot water extraction is completed, stop heating and keep the stirring paddle to continue to work. After the extraction tank is cooled to 60°C, add 1 g of neutral protease to the extraction tank in sequence and stir for 15 minutes, then add 3 g of alkaline protease to shear After cutting for 3 hours, add 0.5 g flavor enzyme to cut for 2 hours. After the reaction is over, raise the temperature of the extraction tank to 95°C and keep it for 30 minutes t...
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