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Application of MOF-Zn fluorescent sensor in detection of chloramphenicol as well as CHL detection method

A fluorescent sensor, chloramphenicol technology, applied in the field of detection of CHL, can solve the problems of high cost, poor repeatability and stability, long antibody development time, etc., and achieve the effect of low detection limit

Inactive Publication Date: 2019-06-14
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunological detection methods have high sensitivity and strong specificity, but the corresponding antibody development time is long and the cost is high, and the repeatability and stability are poor

Method used

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  • Application of MOF-Zn fluorescent sensor in detection of chloramphenicol as well as CHL detection method
  • Application of MOF-Zn fluorescent sensor in detection of chloramphenicol as well as CHL detection method
  • Application of MOF-Zn fluorescent sensor in detection of chloramphenicol as well as CHL detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 prepares MOF-Zn solution:

[0034] First synthesize the ligand bbi: weigh a certain amount of imidazole (3.1-7.5g), NaOH (2.0-8.0g) and urea (3.6-4.5g) in H 2 O, DMA, DMSO, DMF, C 2 h 5 OH, CH 3 OH and other one or several combination solutions (10mL), fully stirred and mixed, reacted in an oil bath at 50-70°C for 1.5-12h, and then weighed a certain amount of 1,1-C 4 h 8 Cl 2 , 1,2-C 4 h 8 Cl 2 , 1,3-C 4 h 8 Cl 2, 1,4-C 4 h 8 Cl 2 , 2,2-C 4 h 8 Cl 2 and 2,3-C 4 h 8 Cl 2 (0.014~0.8mol) one or several combinations are added to the above solution several times, and after 5~8 hours, the solution is immersed in 500mLH 2 O, DMA, DMSO, DMF, C 2 h 5 OH,CH 3 OH and other mixtures of one or more combinations of ice and water, let it stand overnight to obtain a large amount of white needle-shaped product, which was filtered by suction to obtain white needle-shaped crystals, which were dried in a vacuum drying oven for later use.

[0035] Weigh ...

Embodiment 2

[0037] Example 2: MOF-Zn is used as a fluorescent probe for the detection of CHL

[0038] Add 100-150 μL of CHL solution to the MOF-Zn solution, and then add ERY, MTR, AMX, CFM, CED, AZM, CEC, PEN, AZL, FOX, CSU, ATM, CAZ, LIN, AMK, GEN, S XT, CRO. Image 6 The fluorescence of CHL on MOF-Zn solution decreased obviously.

[0039] In MOF-Zn solution, when the amount of CHL increased from 0 μL to 90 μL, the fluorescence intensity of MOF-Zn decreased monotonously and sharply, Figure 7 With the increase of CHL concentration, the fluorescence intensity of MOF-Zn solution was gradually quenched. This indicates that MOF-Zn can be highly sensitive to CHL as a fluorescent probe. By Stern-Volmer (SV) equation: I 0 / I=Ksv[Q]+1 can quantitatively explain the fluorescence quenching efficiency, and can also calculate the detection limit; where I 0 is the fluorescence intensity before the analyte, I is the fluorescence intensity after the analyte, and Ksv is the quenching constant (M -...

Embodiment 3

[0041] Example 3: MOF-Zn is used as a fluorescent probe for the practical detection of CHL

[0042] Get 1ml of commercially available chloramphenicol eye drops (Shandong Bausch & Lomb Freda Pharmaceutical Co., Ltd., containing 2.5 mg of chloramphenicol) and add it to a 100ml volumetric flask to dilute 100 times as the test solution. Take 50 μL of MOF-Zn solution, add 30 μL of the solution to be tested, and add 3 ml of distilled water; take another 50 μL of MOF-Zn solution, add 30 μL of distilled water, and then add 3 ml of distilled water. The two solutions were tested using a spectrofluorometer, from Figure 11 It can be seen that no fluorescence quenching occurred in the blank test, but significant fluorescence quenching occurred after adding a very small amount of chloramphenicol solution.

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Abstract

The invention discloses application of an MOF-Zn fluorescent sensor in detection of chloramphenicol as well as a CHL detection method. The application comprises the steps of taking solution of a metalorganic framework material MOF-Zn, adding to-be-detected solution and testing mixed solution by using a fluorospectrophotometer. When concentration of CHL is increased to 0.01M from 0M, fluorescenceintensity of MOF-Cd monotonously and sharply decreases, which shows that the MOF-Zn can be highly sensitive to CHL when being taken as a fluorescent probe. When the concentration of CHL is increased to 0.071mM, the fluorescence intensity of MOF-Cd is almost completely quenched. Based on standard deviation (sigma) of three times of repeated measurements based on fluorescence intensity of blank solution, a detection limit (3sigma / Ksv) is calculated to be 2.334ppb. 200[mu]L solution of substances like antibiotics is added into the MOF-Zn solution, and then 40[mu]L CHL is added, so that other substances like the antibiotics exist under the condition that CHL exists. After mixed substances (Y+CHL) are added, the fluorescent intensities of all the substances like the antibiotics are quenched, which shows that the probe can selectively identity CHL and the other substances like the antibiotics do not interfere with detection on CHL.

Description

technical field [0001] The invention relates, in particular, to an application of a MOF-Zn fluorescence sensor in detecting chloramphenicol and a method for detecting CHL. Background technique [0002] With growing concerns about public health and water quality, there is now a greater need to detect and remove pollutants from wastewater. Metal ions are important pollutants discharged by chemical companies; chloramphenicol is widely used to treat bacterial infections in humans and animals, and has now become a class of important organic pollutants in water. Abuse of chloramphenicol leads to high levels of chloramphenicol residues. Various types of chloramphenicol have been detected in surface and groundwater, as well as drinking water, and it is important to monitor and remove these specific pollutants from water. Therefore, it is necessary to develop a method for the detection of CHL with high sensitivity. [0003] So far, several methods have been developed for the sensiti...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 牛和林吴笛周国松刘文胜邢鹏程
Owner ANHUI UNIVERSITY
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