A method for in vitro preservation of anthurium germplasm resources

A technology of in vitro preservation and anthurium, which is applied in the field of in vitro preservation of plant germplasm resources, can solve the problems of frequent transfer of tissue culture preservation, susceptibility to the influence of diseases and insect pests, and high cost of planting and preservation, so as to ensure the excellent characteristics of germplasm, The effect of reducing the work of subculture inoculation and saving storage space

Active Publication Date: 2021-10-29
三明市农业科学研究院
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  • Abstract
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Problems solved by technology

[0006] The invention provides a method for in vitro preservation of anthurium germplasm resources, the main purpose of which is to overcome the high cost of planting and preservation of anthurium germplasm resources in greenhouses, which are easily affected by diseases and insect pests, and the normal subculture preservation and transfer of tissue culture are frequent, costly defects

Method used

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  • A method for in vitro preservation of anthurium germplasm resources
  • A method for in vitro preservation of anthurium germplasm resources
  • A method for in vitro preservation of anthurium germplasm resources

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Embodiment 1

[0034] In vitro preservation medium: White+0.1g / LC 6 h 5 Na 3 o 7 +30g / L sucrose+5g / L agar powder;

Embodiment 2

[0036] In vitro preservation medium: White+0.15g / LC 6 h 5 Na 3 o 7 +30g / L sucrose+5g / L agar powder;

Embodiment 3

[0038] In vitro storage medium: White+0.2g / LC 6 h 5 Na 3 o 7 +30g / L sucrose+5g / L agar powder;

[0039] 4) Refer to figure 1 , figure 2 , image 3 . Genetic Stability Identification of Progeny of Preserved Materials: Putting "Crown" Test-tube Plantlets in No Added C 6 h 5 Na 3 o 7 (trisodium citrate) control in vitro preservation medium for 3 months, the plants filled the tissue culture bottle, and most of the leaves turned yellow (such as figure 1 shown), and after 5 months, the plants were senescent and dead. However, the "Crown" test-tube plantlets grew slowly on the in vitro preservation medium that added trisodium citrate, and the plants grew normally after 6 months (such as figure 2 shown), after 16 months, the plants grow taller and the leaves grow up (as shown in image 3 As shown), the stem of the plant is green, only a small amount of leaves in the lower part are withered and yellow, and the whole stem of the plant is usable; it can be seen that the in ...

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Abstract

A method for in vitro preservation of anthurium germplasm resources, using the thick sucking buds of anthurium after removing leaves as explants, washing them under tap water, disinfecting the surface of the explants with 72-75% alcohol, and using Rinse once with sterile water, treat with 0.1% mercuric chloride, rinse three times with sterile water, blot dry with sterile filter paper; then cut the explants into segmented stem segments and inoculate them into bud induction medium for culture After 45 days, transfer the germinated axillary buds to the proliferation medium to obtain clustered seedlings for 40 days; divide the obtained clustered shoots into individual plants and transfer them to the in vitro preservation medium for preservation; in vitro preservation culture Base: White+0.1~0.2g / LC 6 h 5 Na 3 o 7 +30g / L sucrose+5g / L agar powder. The method of the present invention preserves the Anthurium test-tube seedlings for 16 months, the botanical traits and RAPD amplification bands of the progeny will not be different, the preservation material is stable, no genetic variation will occur, and the species degradation and virus infection can be prevented, and the species quality can be guaranteed. Excellent quality characteristics.

Description

technical field [0001] The invention relates to the field of in vitro preservation of plant germplasm resources, in particular to a method for in vitro preservation of anthurium germplasm resources. Background technique [0002] Anthurium andraeanum, also known as Anthurium, is a perennial herbaceous ornamental plant of the family Araceae, native to Colombia. Because of its colorful heart-shaped spathes and gorgeous spadix flowers, it is very popular among consumers all over the world. It has been reported that only Anthura in the Netherlands has thousands of native and cultivated species of anthurium. Since the cultivated anthurium is a heterozygote, the traits of offspring are easy to separate, and the seed life is extremely short, it is difficult to preserve germplasm resources through seeds, and the female parent is mainly preserved through facility cultivation. However, this conventional preservation method consumes a lot of manpower, material resources and resources. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01N3/00
Inventor 莫智龙林辉锋周辉明陈昌铭郭芸玮夏朝水林发壮姚凤琴陈玮婷曹奕鸯
Owner 三明市农业科学研究院
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