Method for purifying hydrolyzed phytosterols by oxidase

Abstract

The present invention discloses a method for purifying hydrolyzed phytosterols by oxidase and relates to the technical field of phytosterols. The method comprises the following steps: hydrolyzing is conducted: vegetable oil is transferred into a hydrolysis kettle, sulfuric acid, sulfonic acid and water are added, and then refluxing is conducted for 1-4 hours; distillate deodorization is conducted:the hydrolyzed vegetable oil is subjected to distillate deodorization, a deodorized distillate is subjected to a methyl esterification reaction, vacuum dehydration is conducted, and then triglycerideis hydrolyzed into fatty acids and glycerol; and after a methyl esterification agent is added, sterol is separated and a filtrate is collected; enzyme catalyzed esterification is conducted; desolvation is conducted; vacuum distillation is conducted; sterol and VE extracting is conducted; separation is conducted; and waste granulation is conducted. The hydrolysis can be realized before the esterification, purification efficiency is improved, and a purification rate can reach 99.3-99.8%. Compared with the existing technology, 1-3 hours can be saved. The method is easy to protect environment effectively, then the waste granulation can be realized after the purification, waste is recycled, and the method is not liable to cause pollution; and at the same time, during the production, waste water is less and the method is conducive to production.

Description

technical field [0001] The invention belongs to the technical field of phytosterols, and in particular relates to a purification method for oxidase hydrolyzing phytosterols. Background technique [0002] Vegetable oil is one of the foods rich in phytosterol content, and the content of phytosterol in corn oil is relatively high. Phytosterols are divided into 4-non-methyl sterols, 4-methyl sterols and 4,4'-dimethyl sterols. The non-methyl sterols mainly include B-sitosterol, stigmasterol, campesterol and brassicasterol, etc. , mainly exists in the seeds of plants. The structure of phytosterols is basically similar to that of animal sterols. The only difference is the number of methyl groups attached to the c-4 position and the difference in the side chains at the c-11 position. It is these side chains Small differences in the chain lead to different physiological functions. [0003] Phytosterols are obtained through physical purification from corn and soybeans, and have the ...

AI Technical Summary

Problems solved by technology

[0007] In order to solve the existing purification method, the reaction speed is slow when the enzyme-catalyzed esterification is carried out during purification, and a large amount of impurities appear in the solution, it is difficult to separate the impurities, the purification efficiency in the later stage is low, and time is wasted; the purpose of the present invention is to provide an oxidation The purification method of enzymatic hydrolysis of phytosterol is convenient and effective for protecting the environment. After purification, waste granulation can be realized, waste can be recycled, and it is not easy to cause pollution; at the same time, there is less waste water during production, and the amount of waste water is 50-50% less than that of the existing process. 60%, conducive to production, while saving time and improving efficiency