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Preparation method of octreotide-coupled nano-chitosan molecular beacon

A nano-chitosan and molecular beacon technology, applied in the field of biomedicine, can solve problems such as poor specificity or stability, harsh process conditions, and complicated preparation methods, and achieve high specificity, high transfection efficiency, and stability Good results

Inactive Publication Date: 2019-07-09
GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a preparation method of octreotide-coupled nano-chitosan molecular beacons for lung cancer diagnosis and treatment, which solves the problem of complex preparation methods and complex processes of drugs or reagents for lung cancer diagnosis and treatment in the prior art. The conditions are harsh and its targeting, specificity or stability is not good, and it can only diagnose the problems of middle and advanced lung cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The nano-chitosan and the molecular beacon were mixed in the PBS buffer of pH 6.0 according to the mass ratio of 7:1, and vortexed for 60 s, and stood at room temperature for 60 min to obtain the nano-chitosan molecular beacon complex; Add 1.0% glutaraldehyde solution dropwise to the nano-chitosan molecular beacon complex, the mass ratio of the glutaraldehyde solution to the nano-chitosan molecular beacon complex is 1000:1, and mix by stirring for the first time Reaction for 1.5h, the stirring speed for the first time is 150rpm, after the reaction, go through the microdialysis column for the first time to dialysis for 1h to remove unbound glutaraldehyde; then according to the mass ratio of octreotide and nano-chitosan molecular beacon complex is 100: Add octreotide at a ratio of 1, carry out the second stirring and mixing reaction for 4 hours, the second stirring speed is 150rpm, after the reaction, the unbound octreotide is removed by the second dialysis of the microdia...

Embodiment 2

[0026] Example 2: The difference from Example 1 is that the chitosan nanometer and the molecular beacon are in a mass ratio of 10:1, and a nano-chitosan molecular beacon complex coupled with octreotide with a particle size of 40nm to 80nm is finally obtained .

Embodiment 3

[0027] Example 3: The difference from Example 1 is that the chitosan nanometer and the molecular beacon are in a mass ratio of 8:1, and finally a nano-chitosan molecular beacon complex coupled with octreotide with a particle size of 45nm to 60nm is obtained .

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Abstract

The invention discloses an octreotide (OCT)-coupled nano-chitosan (CS) molecular beacon (MB) for early diagnosis and treatment of the lung cancer. A nano-chitosan molecular beacon (CS-MB) is synthesized through self-assembly first, and then through chemical reaction, glutaraldehyde is used as a crosslinking agent to synthesize the octreotide-coupled nano-chitosan molecular beacon (CS-MB-OCT). TheCS-MB-OCT nano-composite can dynamically monitor the generation and development of the lung cancer according to the fluorescence intensity, octreotide itself or the composite is combined with microRNAhighly expressed by tumor cells to further suppress the expression of the microRNA, and accordingly, tumor suppression is achieved. The octreotide-coupled nano-chitosan molecular beacon can be applied to lung cancer cells, lung cancer nude mouse transplantation tumors and living animal imaging of early lung cancer transgenic mouse models, and has the advantages of high targeting, high transfection efficiency, high specificity, high stability, low background, high tumor inhibition rate and the like, thereby providing a new idea, a new method and a new technique for early diagnosis and treatment of the lung cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing an octreotide-coupled nano-chitosan molecular beacon used for the diagnosis and treatment of lung cancer. Background technique [0002] Lung cancer is a malignant tumor that seriously threatens human health. Its morbidity and mortality are increasing year by year. Early diagnosis and early treatment are the key to improving the prognosis of patients. However, lung cancer patients often have no obvious symptoms in the early stage of the disease. best time for treatment. Therefore, starting from the "origin" of lung cancer, exploring new methods for efficient and specific early diagnosis and treatment will have a profound impact on the prevention, diagnosis and treatment of lung cancer. [0003] The tumor stem cell theory holds that there is a group of very small tumor cells in tumor tissue cells, called tumor stem cells, and all tumor cells are differen...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K47/61A61K38/08A61P35/00
CPCA61K38/08A61K47/61A61K49/0032A61K49/0054A61P35/00
Inventor 朱海振黄立敏王姿郭熠朱海燕胡建军候净杨飞月蒋莹思雪莹江飞龙
Owner GUIZHOU PROVINCIAL PEOPLES HOSPITAL