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Preparation method and application of flammulina velutipes polysaccharide (FVP)

A technology of Flammulina velutipes polysaccharides and polysaccharides, applied in the fields of application, polysaccharide/gum-containing food ingredients, food ingredients, etc., can solve the problems that have not been seen in relevant reports, and achieve the effects of easy product quality, simple process flow, and easy access

Active Publication Date: 2019-07-09
时代生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report about the effect of Flammulina velutipes polysaccharide FVP on reducing the pathological damage of IBD

Method used

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  • Preparation method and application of flammulina velutipes polysaccharide (FVP)
  • Preparation method and application of flammulina velutipes polysaccharide (FVP)
  • Preparation method and application of flammulina velutipes polysaccharide (FVP)

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preparation example Construction

[0031] The present invention provides a kind of preparation method of Flammulina velutipes polysaccharide FVP, comprising the following steps:

[0032] Step 1: Dry and crush the fruiting bodies of Flammulina velutipes, mix them with 30-40 times the volume of water, then add pectinase at a mass concentration of 0.1-0.3%, enzymolyze at 40-50°C for 1-2h, then use high temperature and freezing Extract polysaccharides by alternate treatment to obtain crude polysaccharide water extract;

[0033] Step 2: Mix the obtained crude polysaccharide aqueous extract with ethanol with a mass fraction of 95% evenly, let stand at 0-10°C for 20-32 hours, centrifuge, collect the precipitate and dry it to obtain the first dry polysaccharide;

[0034] Step 3: The dried first polysaccharide product was subjected to DEAE-Sepharose FF chromatography and G-100 Sephadex gel chromatography in sequence to obtain Flammulina velutipes polysaccharide FVP.

[0035] In the above scheme, the present invention m...

Embodiment 1

[0048] The preparation method of Flammulina velutipes FVP in the prior art comprises: drying and crushing Flammulina velutipes, mixing with 20 times the volume of water, refluxing and extracting at 100°C for 3 times, each time for 2h, filtering, and concentrating the supernatant obtained several times to 100mL; Chloroform:butanol with a volume ratio of 5:1 deproteinized 5 times to obtain a deproteinized polysaccharide solution, then mixed the obtained deproteinized polysaccharide solution with ethanol with a volume fraction of 95%, overnight at 4°C, and centrifuged to collect the precipitate , and then vacuum freeze-dry the resulting precipitate to obtain dry polysaccharides; the resulting dry polysaccharides were chromatographed on DEAE-Sepharose FF, and distilled water and NaCl solutions (0.05M, 0.1M, 0.2M, 0.3M and 0.5 M) Carry out gradient elution as eluent, collect the polysaccharide in distilled water elution part, obtain Flammulina velutipes polysaccharide FVP, wherein u...

Embodiment 2

[0059] The preparation method of Flammulina velutipes polysaccharide FVP comprises:

[0060] 1) Get fresh Flammulina velutipes fruiting bodies to clean, dry in an oven at 45°C to constant weight, pulverize through a 100-mesh sieve, and count in parts by weight, mix 50 parts of crushed Flammulina velutipes fruiting bodies with 40 times the volume of water, mix them by mass Add pectinase at a concentration of 0.3%, enzymolyze at 50°C for 2 hours to obtain an enzymolyzate;

[0061] 2) The obtained enzymolysis solution was treated at 100°C for 60 minutes and filtered, and the filter residue was immediately frozen at -20°C for 1 hour; the frozen filter residue was taken out and mixed with 40 times the volume of water, extracted at 100°C for 60 minutes, and filtered , mixing the supernatants obtained by two times of filtration to obtain a crude sugar aqueous extract;

[0062] 3) The obtained crude polysaccharide aqueous extract was mixed with chloroform:n-butanol at a volume ratio ...

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Abstract

The invention discloses a preparation method of flammulina velutipes polysaccharide (FVP), which comprises the following steps: drying and crushing flammulina velutipes sporocarp, and then mixing thecrushed sporocarp with water with an amount of being 30-40 times of the volume of the crushed sporocarp, then adding pectinase according to a mass concentration of 0.1-0.3% for enzymolysis, and conducting extracting by adopting a high-temperature and freezing alternative treatment mode to obtain a crude polysaccharide water extract; mixing the obtained crude polysaccharide water extract with 95% ethanol, and carrying out standing at 0-10 DEG C for 20-32 hours, then conducting centrifuging, collecting precipitates and conducting drying to obtain a first polysaccharide dry product; conducting chromatography on the first polysaccharide dry product sequentially by DEAE-agarose gel FF and G-100 glucan gel to obtain the flammulina velutipes polysaccharide (FVP). The preparation method can shorten the extraction time and improve the polysaccharide yield. The invention also discloses application of the polysaccharide to a preparation for increasing the content of short-chain fatty acids in intestinal tracts and adjusting the flora structure in the intestinal tracts, so that the polysaccharide has feasibility in preparation of medicines and / or foods for preventing and treating colitis.

Description

technical field [0001] The invention relates to the technical field of polysaccharide activity, more specifically, the invention relates to a preparation method and application of Flammulina velutipes polysaccharide FVP. Background technique [0002] Polysaccharides are one of the important substances that constitute life. They are ubiquitous in plants. Most polysaccharides are biologically active and can regulate the immune system in multiple directions. Flammulina velutiper, also known as structure bacteria, frozen bacteria, intellectual mushrooms, is rich in protein, carbohydrates, mineral elements, cellulose and crude fiber, and low in fat. It is a rare high-nutrition food. It is widely distributed in my country, and its output ranks fourth in the output of edible fungi, and the industrialization ratio has reached 33%, which has a very high level. Flammulina velutipes polysaccharide is one of the main active ingredients of Flammulina velutipes, which has anti-tumor, imm...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P1/00A23L33/125
CPCA23V2002/00A23L33/125A61P1/00C08B37/0003A23V2200/32A23V2250/51
Inventor 刘会平廖文镇叶菊风刘智钧邓玉娣王向东其他发明人请求不公开姓名
Owner 时代生物科技(深圳)有限公司
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