Adenosine deaminase detection kit

A technology of adenosine deaminase and kit, which is applied in the fields of medicine and biochemistry, can solve the problems of low sensitivity and achieve the effects of increased sensitivity, high accuracy and simple operation

Inactive Publication Date: 2019-07-09
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problem of low sensitivity of the existing adenosine deaminase assay kit, the adenosine deaminase assay kit provided by the p

Method used

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Experimental program
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Effect test

Embodiment 1

[0041] The components and concentrations of the adenosine deaminase assay kit of the present embodiment are as follows:

[0042] Reagent R1 includes: purine nucleoside phosphorylase 100.0U / L, xanthine oxidase 300.0U / L, peroxidase 400.0U / L, thimerosal 0.2g / L, 4-aminoantipyrine 0.4mmol / L And 3-morpholine propanesulfonic acid buffer 80.0mmol / L;

[0043] Reagent R2 includes: adenosine 6.0mmol / L, phenol 1.0mmol / L, thimerosal 0.2g / L, 3-morpholinepropanesulfonic acid buffer 80.0mmol / L, polyhexamethyleneguanidine 1.0g / L and β- Cyclodextrin 100.0mg / L;

[0044] The pH values ​​of reagent R1 and reagent R2 are both 6.5.

Embodiment 2

[0046] The components and concentrations of the adenosine deaminase assay kit of the present embodiment are as follows:

[0047] Reagent R1 includes: purine nucleoside phosphorylase 300.0U / L, xanthine oxidase 500.0U / L, peroxidase 800.0U / L, sodium azide 1.0g / L, 4-aminoantipyrine 1.5 mmol / L and Tris buffer 100.0mmol / L;

[0048] Reagent R2 includes: adenosine 20.0mmol / L, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline 3.0mmol / L, sodium azide 1.0g / L, tris Hydroxymethylaminomethane buffer 100.0mmol / L, polyhexamethyleneguanidine 3.0g / L and β-cyclodextrin 500.0mg / L;

[0049] Both reagent R1 and reagent R2 have a pH of 7.5.

Embodiment 3

[0051] The components and concentrations of the adenosine deaminase assay kit of the present embodiment are as follows:

[0052] Reagent R1 includes: purine nucleoside phosphorylase 200.0U / L, xanthine oxidase 400.0U / L, peroxidase 600.0U / L, sodium azide 0.5g / L, 4-aminoantipyrine 1.0 mmol / L and Tris buffer 90.0mmol / L;

[0053] Reagent R2 includes: adenosine 10.0mmol / L, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline 20.0mmol / L, sodium azide 0.5g / L, three Hydroxymethylaminomethane buffer 90.0mmol / L, polyhexamethyleneguanidine 2.0g / L and β-cyclodextrin 300.0mg / L;

[0054] The pH values ​​of reagent R1 and reagent R2 are both 7.0.

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Abstract

The invention belongs to the technical field of medicine and biochemistry and particularly relates to an adenosine deaminase detection kit. The kit comprises a reagent R1 and a reagent R2; the reagentR1 comprises purine nucleoside phosphorylase, xanthine oxidase, peroxidase, a preservative, 4-amino antipyrine and a buffer solution; the reagent R2 comprises adenosine, a color developing agent, a preservative and a buffer solution. The adenosine deaminase detection kit adopts a continuous monitoring method for detection, and the main wavelength is 550 nm; the detection kit has the advantages ofhigh sensitivity and accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to an adenosine deaminase assay kit. Background technique [0002] Adenosine deaminase (adenosine deaminase, ADA) is an important enzyme in purine nucleoside metabolism. Its activity is a sensitive indicator reflecting liver damage and can be used as one of the routine inspection items of liver function. The determination of ADA activity in serum can be used to: judge acute liver injury and residual disease; assist in the diagnosis of chronic liver disease; help in the diagnosis of liver fibrosis; help in the identification of jaundice. In addition, ADA deficiency is related to severe combined immunodeficiency disease (SCID), which can be used as an important enzyme in nucleic acid metabolism; ADA deficiency can lead to nucleic acid metabolism disorders, affect the development of thymus, and cause immune dysfunction. CSF ADA detection can be used as an important...

Claims

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Application Information

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IPC IPC(8): C12Q1/48C12Q1/34C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/34C12Q1/48C12Q2326/96G01N2333/9029G01N2333/908G01N2333/91142G01N2333/978
Inventor 陈青松林耀文李传清
Owner 浙江夸克生物科技有限公司
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