Method for detecting capsaicin compound in grease

A technology of capsaicin and oil, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems of long time-consuming, complicated pre-treatment, and sensitivity that cannot meet the detection of refined oils, etc., and achieve the effect of shortening the detection time and reducing the detection cost

Active Publication Date: 2019-07-09
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for detecting capsaicin mainly include spectrophotometry, chromatography, sensor method, immunological method, etc., but these methods still generally have the problems of complicated pretreatment and long time consumption, and the sensitivity often cannot meet the requirements of refined oil detection. Require

Method used

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  • Method for detecting capsaicin compound in grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Sample pretreatment scheme

[0016] 1. Preparation of immunomagnetic beads:

[0017] (1) Take 1ml of magnetic beads (10mg / ml, product number MS300 / Carboxyl, Japan JSR Company), place the magnetic beads in a magnetic field, collect the magnetic beads, and discard the supernatant;

[0018] (2) Resuspend the magnetic beads with 1ml PBS (phosphate buffered saline, pH 7.4), add 5mg EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), Shake at room temperature for 15 minutes;

[0019] (3) Place the magnetic beads in a magnetic field, collect the magnetic beads, and remove the supernatant; resuspend the particles with 1ml PBS (pH7.4); repeat this step once;

[0020] (4) Add 20 mg of anti-capsaicin antibody and mix at room temperature for 2 hours;

[0021] (5) add blocking agent, continue to react for 2h;

[0022] (6) Place the magnetic beads in a magnetic field, collect the magnetic beads, and remove the supernatant; resuspend the particles with 1ml...

Embodiment 2

[0035] The influence of immunomagnetic bead blocking agents on the detection results: Based on Example 1, different blocking agents (see Table 1) were used to block the magnetic beads. Different blocking agents had a significant impact on the detection sensitivity, the detection limit of 5-aminovaleric acid was the best, and that of deep-water fish gelatin was the worst. Particles sealed with Tris are prone to aggregation in oil-water mixtures, especially in a magnetic field. After aggregation, it is difficult to disperse, making subsequent detection operations impossible.

[0036] Table 1 Effect of blocking agent on the results

[0037] sealant Working concentration of blocking agent (final concentration) Lower detection limit (μg / kg) Remark BSA (Bovine Serum Albumin) 1% (W / V) 60 Deep-water fish gelatin (Sigma company) 0.2% (W / V) 240 PEG20000 (polyethylene glycol 20000) 1% (W / V) 30 Tris (trishydroxymethylaminomethane) 20mM ...

Embodiment 3

[0039] The influence of immunomagnetic bead reconstitution solution on the detection results: Based on Example 1, the influence of different reconstitution solutions (Table 2) on the detection results was compared. It can be seen from the table that the reconstitution solution added with hydrolyzed milk protein is The increase in detection sensitivity has been significantly improved.

[0040] Table 2 Effect of complex solution on detection results

[0041] Numbering formula Lower detection limit (μg / kg) 1 20mM Tris-HCl, 1% BSA, pH7.5 6 2 PBS, 1%BSA, pH7.4 24 3 20mM Tris-HCl, pH7.5 3 4 PBS, 1% hydrolyzed milk protein, pH7.4 1.5

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Abstract

The invention discloses a method for detecting a capsaicin compound in grease. The method for detecting the capsaicin compound in the grease comprises the following steps that capsaicin molecules in the grease are separated and extracted by using an immunoaffinity method in advance, then the capsaicin molecules separated and extracted are detected by using an immunochromatography, using the immuneaffinity method to separate and extract the capsaicin molecules in the grease means using a solution containing methanol and nano-titanium dioxide to dilute a grease sample, and then immunomagnetic beads specific binding to the capsaicin moleculesare added to capture the capsaicin molecules. The method combines immunochromatography with immunoaffinity technique, the workload of detection of capsaicin in the grease is greatly reduced, and the sensitivity of the detection is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting capsaicin in oil. Background technique [0002] "Gutter oil", also known as "swill oil" and "swill oil", generally refers to all kinds of low-quality oils that exist in daily life. cooking oil. However, some unscrupulous traders, driven by profit and disregarding the safety of the people, privately produce and process "gutter oil" and sell it to small restaurants at a low price as edible oil, which has brought serious harm to consumers' health and life safety. However, detecting waste oil is very difficult. Waste oil is an extremely non-standard research object, with no fixed composition and no typical samples, which brings great uncertainty to the detection. The complex and changeable composition makes it impossible to find characteristic indicators for waste oil, and the characteristic indexes of normal edible oil cannot be applied in the detec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/54326G01N33/558
Inventor 李金峰柯跃斌黎诚耀夏俊杰彭朝琼肖云军吕子全何洁
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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