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Application of lactobacillus rhamnosus and compound bacterial powder thereof in products for preventing and treating vaginitis

A technology of Lactobacillus rhamnosus and compound bacteria powder, applied in the direction of Lactobacillus, application, function of food ingredients, etc., can solve the problem of easy recurrence, no recovery of vaginal pH value and quantity and quality of lactobacilli in the vagina, and no real recovery of the vagina. Micro-ecological environment and other problems, to achieve the effect of resisting growth and reproduction and maintaining health

Active Publication Date: 2019-07-16
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the treatment of vaginitis, in the past, antibiotics were simply used to kill pathogenic microorganisms, which was only a superficial cure, and did not restore the vaginal pH value and the quantity and quality of lactobacilli in the vagina, nor did it really restore the destroyed vaginal microecology. environment, so the cure in the true sense has not been achieved, and it is very easy to relapse

Method used

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  • Application of lactobacillus rhamnosus and compound bacterial powder thereof in products for preventing and treating vaginitis
  • Application of lactobacillus rhamnosus and compound bacterial powder thereof in products for preventing and treating vaginitis
  • Application of lactobacillus rhamnosus and compound bacterial powder thereof in products for preventing and treating vaginitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Identification of Lactobacillus rhamnosus LR519

[0027] (1) Strain isolation

[0028] The milk fan (collected from the farmer's home in Jianchuan County, Dali, Yunnan Province) was taken, washed with sterile saline, inserted into skimmed milk with a mass percentage of 12%, and incubated at room temperature until curdling. Take 1 mL of curdled skim milk, dilute it with 9 mL of sterile saline, spread it on MRS solid medium, and incubate at 37°C for 72 hours. Select an MRS plate with a single colony, pick colonies with different sizes, colors, and states, and carry out Gram staining, insert Gram-positive bacilli into MRS liquid medium, and culture at 37°C until turbid. Stretch and separate the bacterial liquid in the MRS solid medium, and repeat this more than 3 times to obtain bacterial colonies and bacterial strains with the same morphology, and store them.

[0029] (2) Strain identification

[0030] The colony was milky white, raised, with ne...

Embodiment 2

[0033] Example 2 Hemolytic test and strain stability evaluation of Lactobacillus rhamnosus LR519

[0034] The results of the hemolysis test showed that Lactobacillus rhamnosus LR519 had no hemolysis, that is, γ hemolysis. The strain stability test showed that after continuous subculture and comparative experiments, no visible changes were found in the strain's morphological characteristics, physical and chemical characteristics, and antibiotic sensitivity, which indicated that the strain's traits could be inherited stably.

Embodiment 3

[0035] Example 3 Lactobacillus rhamnosus LR519 and Lactobacillus plantarum LP45 antibacterial ability test

[0036] (1) Inhibitory ability test to Escherichia coli and Staphylococcus aureus

[0037] (1) Test method

[0038] ① Lactobacillus rhamnosus LR519 and Lactobacillus plantarum LP45 were activated by cryopreservation tubes, respectively inoculated in liquid MRS medium to activate for 3 generations, and the activated lactic acid bacteria were inoculated into liquid MRS medium at 3% inoculum, and kept at 37°C Cultivate for 24 hours, centrifuge the lactic acid bacteria fermentation liquid at 8000 rpm / min for 10 minutes to collect the fermentation supernatant, and use a bacterial filter to filter and sterilize the supernatant for later use;

[0039] ②Pour 10mL of 2.0% plain agar medium cooled to 50℃ into a sterile plate and dry it in a clean bench;

[0040] ③Put the sterilized Oxford cup on the plain agar medium and let it stand for 10 minutes;

[0041] ④Add 1mL cultured E...

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PUM

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Abstract

The invention discloses novel lactobacillus rhamnosus, namely lactobacillus rhamnosus LR519 which has the preservation number of CGMCC No.15969. The strain has a relatively good effect of inhibiting escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans and other vaginal pathogenic bacteria, can be used for regulating the vaginal microecological balance and maintaining health of female vaginae, can be used for preparing medicines, foods and hygienic products for preventing and treating vaginitis. The invention further discloses compound bacterial powder containing thelactobacillus rhamnosus LR519 and lactobacillus plantarum LP45 and application thereof in medicines, foods and hygienic products for preventing and treating vaginitis.

Description

technical field [0001] The present invention relates to a new Lactobacillus rhamnosus LR 519, and at the same time relates to bacteria powder containing Lactobacillus rhamnosus LR 519 and its use. Background technique [0002] Vaginitis, that is, vaginal dysbiosis, is a disease that seriously affects the physical and mental health of women of childbearing age. The main clinical manifestations are abnormal leucorrhea and genital itching. Clinically common vaginitis is mainly divided into four categories, namely bacterial vaginosis, vulvovaginal candidiasis, trichomonas vaginitis, and senile vaginitis. [0003] For the treatment of vaginitis, in the past, antibiotics were simply used to kill pathogenic microorganisms, which was only a superficial cure, and did not restore the vaginal pH value and the quantity and quality of lactobacilli in the vagina, nor did it really restore the destroyed vaginal microecology. environment, so the cure in the true sense has not been achieved...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61K9/14A61P15/02A61P31/04A61P31/10A23L33/135C12R1/225
CPCC12N1/20A61K35/747A61K9/14A61P15/02A61P31/04A61P31/10A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2400/169A61K2300/00A23V2200/324Y02A50/30
Inventor 赵林森鄢梦洁李华文张欢欢齐世华杨玉红路江浩孙新凯
Owner 河北一然生物科技股份有限公司
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