Bacillus velezensis, and micro-ecological preparation and preparation method thereof

A technology of Bacillus velaisi and microecological preparations, applied in the field of microecological preparations, can solve the disease prevention and control effect of freshwater fish that has not been seen, the prevention and control of fish diseases that have not seen Bacillus velaisi, and freshwater products that have not been seen Fish pathogenic bacteria have antagonistic effects and other problems, achieving good antibacterial effect and good biological control effect

Active Publication Date: 2019-07-16
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

[0005] Although there have been many research reports on Bacillus velezensis as an important biocontrol bacterium and probiotic, and there are also research reports on the antagonism of pathogenic bacteria in seawater fish, but so far, it has not been seen Involving the research reports that the pat

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  • Bacillus velezensis, and micro-ecological preparation and preparation method thereof
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  • Bacillus velezensis, and micro-ecological preparation and preparation method thereof

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Embodiment Construction

[0043] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0044] The chemical reagents used in the examples of the present invention were obtained through conventional commercial channels unless otherwise specified.

[0045] (1) Collection, isolation, purification and screening process of Bacillus veleisi BvL03

[0046] Collect sediment samples from fish ponds in Qiaokou Town, Wangcheng District, Changsha City, Hunan Province, weigh 5 g of sediment and add it to 45 mL of sterile water, shake it with a vortex shaker, and then dilute it with a 10-fold gradient dilution method. diluted to 10 -4 g / mL, 10 -5 g / mL, 10 -6 g / mL, use a pipette to take 100 μL onto the LB plate, spread it evenly with a disposable spreader, set up 3 parallels, culture at 30 ℃ overnight, pick a single clone for purification, and purify 3 times, Pick a single clone of the purified strain and insert it into LB liquid medium, c...

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Abstract

The invention relates to a Bacillus velezensis, and a micro-ecological preparation and preparation method thereof. The Bacillus velezensis is preserved in China Center for Type Culture Collection, hasa strain preservation number of CCTCC NO: M 2019218, and is classified and named as Bacillus velezensis BvL03, and the Latin academic name is Bacillus velezensis BvL03. The micro-ecological preparation suitable for aquiculture prepared by the strain has broad-spectrum effect of inhibiting fish pathogenic bacteria through drug-delivery routes such as feed addition, water body sprinkling or intraperitoneal injection, and the protection rate reaches 85 percent. After acting on fish bodies, the preparation can effectively prevent fish pathogenic bacteria such as aeromonas hydrophila from infecting freshwater fishes such as grass carps and crucian carps, and particularly has the best protection effect on grass carps.

Description

technical field [0001] The invention relates to the field of microecological preparations, in particular to a strain of Bacillus velez, its probiotics and a preparation method thereof. Background technique [0002] In recent years, in the process of aquaculture, bacterial diseases have occurred frequently and spread widely. Among them, explosive hemorrhagic disease is the most harmful to fish. Once this disease breaks out, it will cause very serious economic losses. However, the prevention and treatment of bacterial diseases mainly relies on antibiotics and chemical drugs, but the methods and dosages used are unscientific, and there are also many hidden dangers, such as the emergence of mutant drug-resistant strains, the destruction of the ecological environment of water bodies, and disturbance of the intestinal tract of aquaculture animals. The ecological balance of microorganisms can cause hidden dangers in the safety of aquatic products and reduce the immunity of aquacul...

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P31/04A23K10/18A23K50/80C12R1/07
CPCC12N1/20A61P31/04A23K10/18A23K50/80A61K2035/115C12R2001/07C12N1/205Y02A40/818
Inventor 夏立秋曹丽娜易敢峰丁学知
Owner HUNAN NORMAL UNIVERSITY
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