Bacillus velezensis, and micro-ecological preparation and preparation method thereof
A technology of Bacillus velaisi and microecological preparations, applied in the field of microecological preparations, can solve the disease prevention and control effect of freshwater fish that has not been seen, the prevention and control of fish diseases that have not seen Bacillus velaisi, and freshwater products that have not been seen Fish pathogenic bacteria have antagonistic effects and other problems, achieving good antibacterial effect and good biological control effect
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[0043] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.
[0044] The chemical reagents used in the examples of the present invention were obtained through conventional commercial channels unless otherwise specified.
[0045] (1) Collection, isolation, purification and screening process of Bacillus veleisi BvL03
[0046] Collect sediment samples from fish ponds in Qiaokou Town, Wangcheng District, Changsha City, Hunan Province, weigh 5 g of sediment and add it to 45 mL of sterile water, shake it with a vortex shaker, and then dilute it with a 10-fold gradient dilution method. diluted to 10 -4 g / mL, 10 -5 g / mL, 10 -6 g / mL, use a pipette to take 100 μL onto the LB plate, spread it evenly with a disposable spreader, set up 3 parallels, culture at 30 ℃ overnight, pick a single clone for purification, and purify 3 times, Pick a single clone of the purified strain and insert it into LB liquid medium, c...
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