Application of combination of chemical mutagenesis method and negative pressure method in chionanthus retusus polyploid breeding

A technology of polyploid breeding and chemical mutagenesis, applied in the fields of application, botany equipment and methods, liquid fertilizer, etc., can solve the problems of poor stress resistance of tassel trees and poor survival rate of seedlings, etc., to promote the rooting of tassel seedlings, The effect of high plant shape and thick trunk

Active Publication Date: 2019-07-23
TAISHAN RES INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the fringe tree that obtains in the above-mentioned technology is poor in stress resistance, and the seedling survival rate after raising seedlings is relatively poor

Method used

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  • Application of combination of chemical mutagenesis method and negative pressure method in chionanthus retusus polyploid breeding
  • Application of combination of chemical mutagenesis method and negative pressure method in chionanthus retusus polyploid breeding
  • Application of combination of chemical mutagenesis method and negative pressure method in chionanthus retusus polyploid breeding

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of chemical mutagenesis method is combined with the application of negative pressure method in tassel tree breeding, comprises the following steps:

[0036] (1) Collect the tassel tree species, bud out through sand storage, place the sprouted seedlings in a petri dish equipped with colchicine solution, and the mass concentration of colchicine in the mutagen is respectively 0.01%; place the petri dish in a negative pressure pump Inside, under the condition of negative pressure value -95KPa, treat for 10 minutes, after the treated germinated seedlings are washed with sterile water, when they grow to 4-5 leaves, they are transplanted;

[0037](2) The above-mentioned fringe saplings obtained above are planted in the matrix soil composed of 100 parts of garden soil, 1 part of vermiculite, 3 parts of inorganic fertilizers, 0.75 parts of sawdust, 2 parts of plant ash, and 0.1 part of rector stone powder. After planting, spray Apply nutrient solution to the leaves, and o...

Embodiment 2

[0040] A kind of chemical mutagenesis method is combined with the application of negative pressure method in tassel tree breeding, comprises the following steps:

[0041] (1) collect tassel tree species, hide buds through sand, place the sprouted seedlings in a petri dish equipped with colchicine solution, and the mass concentration of colchicine in the mutagen is respectively 0.3%; place the culture medium in a negative pressure pump Inside, under the condition of negative pressure value-95KPa, treat for 10min, after the germinated seedlings grow 4-5 leaves after treatment, carry out transplanting;

[0042] (2) The above-mentioned fringe saplings obtained above are planted in the matrix soil composed of 100 parts of garden soil, 5 parts of vermiculite, 6 parts of inorganic fertilizer, 1.25 parts of sawdust, 3 parts of plant ash, and 0.6 part of rector stone powder. After planting, spray Apply nutrient solution to the leaves, and one week after transplanting, spray liquid fert...

Embodiment 3

[0045] A kind of chemical mutagenesis method is combined with the application of negative pressure method in tassel tree breeding, comprises the following steps:

[0046] (1) Collect the tassel tree species, germinate through sand storage, place the sprouted seedlings in a petri dish equipped with colchicine solution, and the mass concentration of colchicine in the mutagen is respectively 0.05%; place the culture medium under negative pressure In the pump, under the condition of negative pressure value -95KPa, treat for 10 minutes, after the treated sprouted seedlings grow 4-5 leaves, transplant them;

[0047] (2) The above-mentioned fringe saplings obtained above are planted in the matrix soil composed of 100 parts of garden soil, 2 parts of vermiculite, 4 parts of inorganic fertilizers, 1 part of sawdust, 2.5 parts of plant ash, and 0.2 part of rector stone powder. After planting, spray Apply nutrient solution to the leaves, and one week after transplanting, spray liquid fer...

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Abstract

The invention discloses application of a combination of a chemical mutagenesis method and a negative pressure method in chionanthus retusus polyploid breeding. The application includes following steps: collecting chionanthus retusus seeds, germinating through sand storage, putting germinating seedlings into a culture dish with a mutagenic agent, putting the culture dish into a negative pressure pump, treating under negative pressure, and transplanting when the treated germinating seedlings grow to have about 4-5 leaves; spraying nutritional liquid to the leaves after transplanting, spraying liquid fertilizer to roots of chionanthus retusus seedlings, correcting chionanthus retusus plants at proper time, and timely removing diseased stems and weak stems; timely preventing and controlling pest and disease when chionanthus retusus seedlings grow. The method can increase probability of chromosome doubling; formed polyploid plants are thicker in leaf, darker in leaf color, bigger in air hole size and fewer in air hole in unit leaf area, and number of guard cell chloroplasts is obviously increased.

Description

[0001] Technical field: [0002] The invention relates to the field of plant breeding, in particular to the application of a combination of a chemical mutagenesis method and a negative pressure method in polyploid breeding of tassel trees. [0003] Background technique: [0004] Tassel tree: It is a plant of the genus Tassel of Oleaceae. It is a deciduous shrub or tree. The tree is tall and graceful, with lush branches and leaves. It is full of white flowers in early summer, like frost and snow, beautiful and pleasant. Autumn results, drupe oval, blue-black. Looking closely, the leaves of the tassel tree are single leaves opposite, the leaves are oval or oblong, entire, and nearly leathery. Dioecious, panicle born at the top of side branches; corolla white, 4-parted, lobes linear oblanceolate, 2 stamens in male flowers, 2-lobed stigma in female flowers. The flowering period is June-July, and the fruiting period is September-October. Because the small flowers of tassel tree a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01G17/00A01G24/10A01G24/15A01G24/23A01C21/00A01G24/12C05G3/00C05G1/00
CPCA01C21/005A01G17/005A01H1/08A01G24/10A01G24/12A01G24/15A01G24/23C05B7/00C05G3/00C05G5/20C05G5/23C05C5/00C05C9/00C05D9/00
Inventor 张林朱翠翠王峰孙忠奎张安琪程甜甜于永畅谢学阳李承秀任红剑
Owner TAISHAN RES INST OF FORESTRY
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