Stem cell freezing and storing medium and preparation method and freezing and storing method thereof
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A cryopreservation method and stem cell technology, applied in the field of stem cell cryopreservation and its preparation, can solve the problems of inability to meet the clinical needs of stem cell therapy, serum contamination, etc., to prevent the risk of contamination and allergens, reduce negative effects, and be safe. high sex effect
Active Publication Date: 2019-07-26
广州赛隽生物科技有限公司
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Problems solved by technology
However, the addition of serum has the risk of introducing contamination and allergens, which cannot meet the clinical needs of stem cell therapy
Method used
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preparation example Construction
[0042] The present invention also provides a preparation method of Plasma-Lyte A electrolyte injection, which comprises the following steps:
[0043] (1) Use an electronic balance to accurately weigh 5.26g of USP grade sodium chloride (NaCl), 0.37g of USP grade potassium chloride (KCl), and 0.37g of USP grade magnesium chloride hexahydrate (MgCl). 2 ·6H 2 O) 0.3g, pharmacopoeia grade sodium gluconate (C 6 h 11 NaO 7 )5.02g, pharmacopoeia grade sodium acetate trihydrate (C 2 h 3 NaO 2 ·3H 2 O) 3.68g;
[0044] (2) Add the auxiliary materials weighed in step (1) into a 1L beaker in turn, add 900mL of ultrapure water into the beaker, fully dissolve, and configure it into an aqueous solution;
[0045] (3) Measure the pH of the aqueous solution obtained in step (2) with a pH meter, and adjust the pH of the aqueous solution to 7.2 to 7.4, and finally set the volume to 1 L;
[0046] (4) Divide the aqueous solution obtained in step (3) into several 500mL blue cap reagent bottl...
Embodiment 1
[0056] A stem cell cryopreservation liquid, which is composed of the following components by weight: 1 part of trehalose, 0.2 parts of dextran 40, 3 parts of human serum albumin, 3 parts of dimethyl sulfoxide, 5 parts of hydroxyethyl starch and Maili A injection 87.8 parts.
Embodiment 2
[0058] A stem cell cryopreservation liquid, which is composed of the following components by weight: 1 part of trehalose, 0.5 parts of dextran 40, 5 parts of human albumin, 5 parts of dimethyl sulfoxide, 6 parts of hydroxyethyl starch and Maili A injection 82.5 parts.
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Abstract
The invention discloses a stem cell freezing and storing medium and a preparation method and freezing and storing method thereof. The stem cell freezing and storing medium comprises, by weight, 3-10 parts of dimethyl sulfoxide, 2-7 parts of human serum albumin, 0.5-3 parts of mycose, 0.2-2 parts of dextran 40 and 2-6 parts of hetastarch. Cells can be frozen for a long time by the stem cell freezing and storing medium, freezing damage of the cells can be remarkably reduced, the resuscitated cells have a high degree of survival rate and adherent property, and freezing and storing effect of the cells is improved; meanwhile, the compositions of the stem cell freezing and storing medium are clear and are medical compendial injection-grade excipients without containing serum, the risk of contamination and allergen introduced by the use of heterogeneous serum is effectively prevented, the content of DMSO (dimethylsulfoxide) is low, the negative effect of the DMSO on the cells is lowered, highsecurity and good stability are achieved, requirements of CFDA (China food and drug administration) and FDA (food and drug administration) are met, and the stem cell freezing and storing medium can be directly used in human infusion and is suitable for clinical research and treatment.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a stem cell cryopreservation solution, a preparation method and a cryopreservation method thereof. Background technique [0002] Stem cells are a type of cells with self-renewal and multi-directional differentiation potential, which have been widely used in scientific research and clinical treatment. Stem cells have broad application prospects in cell therapy, regenerative medicine, drug discovery, toxicology, and developmental biology. At present, there are stem cell drug products on the market all over the world. With the development of science and technology, stem cells will be more effectively and widely used in the treatment of various diseases to make up for the ineffectiveness of traditional drug treatments for certain diseases. [0003] During the preparation or clinical application of stem cells, the main storage method is to store them in liquid nitrogen at low temperature...
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