Pharmaceutical composition for enhancing immune function comprising enzyme treated royal jelly powder as an active ingredient
A technology to enhance immunity and active ingredients, which is applied in the direction of medical preparations containing active ingredients, drug combinations, powder delivery, etc., can solve the problems of unexplained enzyme treatment of royal jelly powder to enhance immune function, etc., to achieve excellent immune function enhancement effect, anti-inflammatory Excellent oxidation and anti-inflammatory activity, excellent activity effect
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Embodiment 1
[0072] Embodiment 1. the preparation of enzyme treatment royal jelly powder
[0073] More than 5.0% of 1kg freeze-dried royal jelly powder (10-hydroxy-2-decenoic acid) relative to 100 parts by weight; Zhejiang Jiangshan Bee Industry Co., Ltd. (ZHEJIANG JIANGSHAN BEEENTERPRISE CO.,LTD) ) after adding 1000 parts by weight of purified water and mixing, simultaneously mixing fungal endoprotease, probeef 2000P as exoprotease, Prozyme 2000P as fungal exoprotease and Foodpro Alkaline protease as bacterial alkaline endoprotease . Each protease was purchased from Vision Biochem (Korea) and mixed to 1 part by weight (total 3 parts by weight) relative to 100 parts by weight of the above-mentioned royal jelly powder. Then the temperature conditions for the first treatment at a temperature of 55°C, the second treatment at a temperature of 45°C, the third treatment at a temperature of 50°C, and the fourth treatment at a temperature of 55°C The reaction was carried out at a temperature of ...
experiment example 1
[0074] Experimental example 1. Using protein electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)) to confirm the presence of allergens (allergens)
[0075] In order to analyze whether there are proteins causing allergic reactions in the enzyme-treated royal jelly powder obtained in the above Example 1, the protein concentration was determined and separated by molecular weight by sodium dodecyl sulfate polyacrylamide gel electrophoresis at a gel concentration of 10% protein. After electrophoresis, the gel was stained with 1% Coomassie Blue (Coomassie Blueg-250), and the non-stained part was decolorized in a decolorizing solution (45% methanol, 10% acetic acid). figure 1 The result is shown. For comparison, as in Example 1, non-enzyme-treated royal jelly (RJ) without enzyme treatment was used at the same time.
[0076] like figure 1 As shown, protein expression of 47-55 kDa was observed in non-enzyme-treated royal jelly, but not in enzyme-tre...
experiment example 2
[0077] Experimental example 2. Proliferation effect of spleen cells and macrophages based on enzyme treatment of royal jelly powder
[0078] 2-1. Spleen cell proliferation effect of enzyme-treated royal jelly powder
[0079] In order to analyze the spleen cell proliferation ability of the enzyme-treated royal jelly powder obtained in the above-mentioned Example 1, the spleen cells obtained from normal BALB / c mice were divided into 5×10 5 The cells (cells) / well were inoculated in a 48-well plate, and after the samples were treated according to the concentration, they were cultured for 24 hours and 48 hours. Then, 250 μg / mL of 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT, 3-(4,5-dimethylthiazol-2-yl) -2-5-diphenytetrazolium bromide) solution, after culturing for 4 hours, remove the culture medium, and add 200 μL of dimethyl sulfoxide (DMSO, dimethyl sulfoxide) to react at room temperature for 10 minutes, and under the condition of 570nm The absorbance was mea...
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