Pharmaceutical composition for enhancing immune function comprising enzyme treated royal jelly powder as an active ingredient

A technology to enhance immunity and active ingredients, which is applied in the direction of medical preparations containing active ingredients, drug combinations, powder delivery, etc., can solve the problems of unexplained enzyme treatment of royal jelly powder to enhance immune function, etc., to achieve excellent immune function enhancement effect, anti-inflammatory Excellent oxidation and anti-inflammatory activity, excellent activity effect

Inactive Publication Date: 2019-08-02
拜特克有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As mentioned above, various pharmacological effects related to royal jelly are known, but until now it has not been elucidated whether enzyme-treated royal jelly powder has the effect of enhancing immune function, and it is in a state of research on this

Method used

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  • Pharmaceutical composition for enhancing immune function comprising enzyme treated royal jelly powder as an active ingredient
  • Pharmaceutical composition for enhancing immune function comprising enzyme treated royal jelly powder as an active ingredient
  • Pharmaceutical composition for enhancing immune function comprising enzyme treated royal jelly powder as an active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1. the preparation of enzyme treatment royal jelly powder

[0073] More than 5.0% of 1kg freeze-dried royal jelly powder (10-hydroxy-2-decenoic acid) relative to 100 parts by weight; Zhejiang Jiangshan Bee Industry Co., Ltd. (ZHEJIANG JIANGSHAN BEEENTERPRISE CO.,LTD) ) after adding 1000 parts by weight of purified water and mixing, simultaneously mixing fungal endoprotease, probeef 2000P as exoprotease, Prozyme 2000P as fungal exoprotease and Foodpro Alkaline protease as bacterial alkaline endoprotease . Each protease was purchased from Vision Biochem (Korea) and mixed to 1 part by weight (total 3 parts by weight) relative to 100 parts by weight of the above-mentioned royal jelly powder. Then the temperature conditions for the first treatment at a temperature of 55°C, the second treatment at a temperature of 45°C, the third treatment at a temperature of 50°C, and the fourth treatment at a temperature of 55°C The reaction was carried out at a temperature of ...

experiment example 1

[0074] Experimental example 1. Using protein electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)) to confirm the presence of allergens (allergens)

[0075] In order to analyze whether there are proteins causing allergic reactions in the enzyme-treated royal jelly powder obtained in the above Example 1, the protein concentration was determined and separated by molecular weight by sodium dodecyl sulfate polyacrylamide gel electrophoresis at a gel concentration of 10% protein. After electrophoresis, the gel was stained with 1% Coomassie Blue (Coomassie Blueg-250), and the non-stained part was decolorized in a decolorizing solution (45% methanol, 10% acetic acid). figure 1 The result is shown. For comparison, as in Example 1, non-enzyme-treated royal jelly (RJ) without enzyme treatment was used at the same time.

[0076] like figure 1 As shown, protein expression of 47-55 kDa was observed in non-enzyme-treated royal jelly, but not in enzyme-tre...

experiment example 2

[0077] Experimental example 2. Proliferation effect of spleen cells and macrophages based on enzyme treatment of royal jelly powder

[0078] 2-1. Spleen cell proliferation effect of enzyme-treated royal jelly powder

[0079] In order to analyze the spleen cell proliferation ability of the enzyme-treated royal jelly powder obtained in the above-mentioned Example 1, the spleen cells obtained from normal BALB / c mice were divided into 5×10 5 The cells (cells) / well were inoculated in a 48-well plate, and after the samples were treated according to the concentration, they were cultured for 24 hours and 48 hours. Then, 250 μg / mL of 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT, 3-(4,5-dimethylthiazol-2-yl) -2-5-diphenytetrazolium bromide) solution, after culturing for 4 hours, remove the culture medium, and add 200 μL of dimethyl sulfoxide (DMSO, dimethyl sulfoxide) to react at room temperature for 10 minutes, and under the condition of 570nm The absorbance was mea...

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PUM

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Abstract

The invention relates to a pharmaceutical composition for enhancing immune function comprising enzyme treated royal jelly (ERJ) powder as an active ingredient. The enzyme-treated royal jelly (ERJ) powder of the present invention does not contain proteins that cause anaphylactic reactions. The composition has excellent antioxidant and anti-inflammatory activities, can actively proliferate immune cells, can induce excellent activity of natural killer cells, has excellent immune function enhancing effects, and can be effectively used as a medicine or a health-care functional food for enhancing immune functions.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for enhancing immune function comprising Enzyme treated royal jelly (Enzyme treated royal jelly, ERJ) powder as an active ingredient. Background technique [0002] As modern people pay more attention to health and quality of life, such as cancer, immunodeficiency, atopic autoimmunity and other immune-related diseases continue to increase, the research and products of health-care functional foods for enhancing immunity are on the rise trend. Therefore, despite the economic downturn at home and abroad, the domestic health functional food market in 2013 was 1,792 billion yuan, an increase of 5.2% over the previous year, and the average annual growth rate in the past five years was 11.5%, showing a continuous growth trend . [0003] In addition, in terms of health functional foods, functional red ginseng, chlorella, and other products related to immune function improvement account for 25%, acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/644A61K9/14A61P37/04A23L33/00A23L21/20A23L5/20A23K10/14A23K10/20
CPCA61K35/644A61K9/14A61P37/04A23L21/20A23L5/25A23L33/00A23K10/14A23K10/20
Inventor 李道行孙姸暻车芝润李娜英金雅凛灿
Owner 拜特克有限公司
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