Single-molecule detection method based on dynamic Raman spectrum

A single-molecule detection and Raman spectroscopy technology, used in Raman scattering, material analysis by optical means, measurement devices, etc., can solve the problems of irreversibility, insufficient resolution, uncontrollability, etc., to achieve intuitive data, use The effect of wide range and good nanoelectrode properties

Inactive Publication Date: 2019-08-02
深圳市新零壹科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a combination voltage regulation based A New Method for Detection of Molecular Interactions by Dynamic Raman Spectroscopy

Method used

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  • Single-molecule detection method based on dynamic Raman spectrum
  • Single-molecule detection method based on dynamic Raman spectrum
  • Single-molecule detection method based on dynamic Raman spectrum

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preparation example Construction

[0050] In step 1), the specific method for preparing the gold electrode can be: place a platinum wire coil with a diameter of about 1 cm (the diameter of the platinum wire is 0.1 mm) on the liquid surface of the HCl and ethanol electrolyte with a volume ratio of 1:1, and place A gold wire with a diameter of 0.2mm is placed at the center of the platinum wire circle, and the tip is about 1mm below the liquid surface. The gold wire is connected to the positive pole and the platinum wire circle is connected to the negative pole. The bottom is just in contact with the liquid surface of the electrolyte, and a 4.2KHz 13.5V square wave is applied, and the gold wire is quickly lifted after 0.5 to 1.5 seconds to complete the secondary etching, and a gold nano-electrode with a relatively uniform tip size is obtained.

[0051] 2) Cleaning of gold electrodes;

[0052] In step 2), the specific method of cleaning the gold electrode can be: put the etched end of the etched gold electrode upsi...

Embodiment 1

[0069] Example 1, see Figure 1 to Figure 5 :

[0070] The invention provides a single-molecule detection method based on dynamic Raman spectroscopy, comprising the following steps:

[0071] Soak the polytetrafluoroethylene-wrapped gold electrode in a 5mM ethanol solution of p-carboxythiophenol for 7 hours, then wash with ethanol and ultrapure water three times each to remove excess molecules to be tested on the surface of the gold electrode. Add 7 μL 10mM p-carboxythiophenol ethanol solution to 1000 μL 150pM 40nm gold nanosphere solution, incubate at room temperature for 12 hours, centrifuge the resulting solution at 5000r / min to remove the supernatant and wash with ultrapure water, repeat three times to obtain p-carboxythiophenol Phenol-modified 40nm gold nanosphere solution. Clean the electrolytic cell, install the gold nanoelectrodes, and add 90 μL of 5 mM PBS and 3 mM K to the electrolytic cell 4 Fe(CN) 6 Then add 30 μL of p-carboxythiophenol-modified 40nm gold nanosp...

Embodiment 2

[0073] Example 2, see figure 1 and Figure 6 :

[0074] The invention provides a single-molecule detection method based on dynamic Raman spectroscopy, comprising the following steps:

[0075] Soak the polytetrafluoroethylene-wrapped gold electrode in 5mM ethanol solution of p-aminothiophenol for 7h, then wash with ethanol and ultrapure water three times respectively to remove excess molecules to be tested on the surface of the gold electrode. Add 7 μL 10mM p-carboxythiophenol ethanol solution to 1000 μL 150pM 40nm gold nanosphere solution, incubate at room temperature for 12 hours, centrifuge the resulting solution at 5000r / min to remove the supernatant and wash with ultrapure water, repeat three times to obtain p-carboxythiophenol Phenol-modified 40nm gold nanosphere solution. Clean the electrolytic cell, install the gold nanoelectrodes, and add 90 μL of 5 mM PBS and 3 mM K to the electrolytic cell 4 Fe(CN) 6 Then add 30 μL of p-carboxythiophenol-modified 40nm gold nanos...

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Abstract

The invention discloses a single-molecule detection method based on dynamic Raman spectrum and relates to a dynamic Raman spectrometry method. The method comprises the following specific steps: modifying a molecule to be measured with a Raman signal at the tip; establishing a single-molecule Raman signal detection system; when a sample is irradiated by excitation light, generating the Raman signal, and detecting interaction between molecules by dynamically detecting change of the Raman spectrum; and adjusting bias voltage on a gold electrode, and monitoring change in intensity of the peaks at1003 cm <-1> and 1027 cm <-1> to control the interaction between molecules. The method can change the distance of metal-metal nano-gap by adjusting the bias voltage to regulate the weak interaction between the molecules, and can modify different signal molecules on the gold electrode and nanoparticles to study interaction between different molecules; and the method is wide in application range, high in function, visual in data and simple to operate.

Description

technical field [0001] The invention relates to image super-resolution realization technology, in particular to a single-molecule detection method based on dynamic Raman spectroscopy, that is, a new method for voltage-regulated single-molecule measurement and its application in detecting the interaction between molecules. Background technique [0002] Currently, the reversible assembly of biomacromolecules (such as nucleic acids, proteins, carbohydrates, and lipids) has stimulated the study of non-covalent intermolecular interactions (such as van der Waals forces, metal atom coordination, π-π interactions and hydrogen bonding) in-depth study. These weakly interacting natural biomacromolecules are the basis for molecular recognition and structural conformation. An understanding of these weak noncovalent bonds is important for applications such as drug design, self-assembly of molecular layers on metal surfaces, and synthesis of novel functional materials. At the same time, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65G01N21/658G01N2021/655
Inventor 梁峰
Owner 深圳市新零壹科技有限公司
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