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Composition with enzymatic anti-inebriation effect and preparation method and application thereof

A technology of composition and function, which is applied in the field of enzyme-catalyzed anti-alcoholism composition and its preparation, can solve the problems of unreported, difficult to decompose alcohol, lack of alcohol, etc., and achieve the reduction of alcohol content, quick effect, and comprehensive prescription Effect

Pending Publication Date: 2019-08-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So, is there any way for us to enjoy the pleasure of alcohol while preventing alcohol from harming our bodies? At present, the research on anti-alcoholic drugs in my country mainly focuses on traditional Chinese medicine or herbal preparations.
However, these preparations usually only have liver protection function, and it is difficult to break down alcohol
Also, some medications even claim to reduce blood alcohol levels, but evidence for this is lacking
At present, there is no report on the preparation that really has the effect of directly decomposing alcohol

Method used

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  • Composition with enzymatic anti-inebriation effect and preparation method and application thereof
  • Composition with enzymatic anti-inebriation effect and preparation method and application thereof
  • Composition with enzymatic anti-inebriation effect and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0043] Cloning (yeast), expression, purification of embodiment 1 alcohol dehydrogenase

[0044] 1. Plasmid construction. The ADH1B2 gene (corresponding NCBI accession number CN20036-1) on the commercial plasmid ADH1B2-pPIC9k (purchased from Jiangsu Hongxun Biotechnology Co., Ltd., containing human ADH1B2 gene and N-terminal-His×6 tag) Amplified by PCR (upstream primer ADH1B2-pPIC3.5k-F: aattattcgaaggatccATGGCCACCATGCACCACCACCATCACCA, downstream primer ADH1B2-pPIC3.5k-R: ccgccctagggaattcTCAAAACGTCAGGACGGTACG), after obtaining the target fragment (including ADH1B2 and His×6 tags), use homologous recombinase ⅡOne StepCloning Kit, the human alcohol dehydrogenase ADH1B2 gene and the His×6 tag were constructed between the BamHI and EcoR I restriction sites of the pPIC3.5k plasmid to obtain the expression vector ADH1B2-pPIC3.5k.

[0045] 2. Construction of a yeast expression system. The plasmid ADH1B2-pPIC3.5k obtained in step 1 was digested with Sac I fast enzyme and transformed in...

Embodiment 2

[0049] The cloning (yeast), expression and purification of acetaldehyde dehydrogenase are similar to the above method.

[0050] 1. Plasmid construction. The ALDH2 gene (corresponding NCBI accession number CNCN19443-1) on the commercial plasmid ALDH2-pPIC9k (purchased from Jiangsu Hongxun Biotechnology Co., Ltd., containing human ALDH2 gene and N-terminal-His×6 tag) Amplified by PCR technology (upstream primer ALDH2-pPIC3.5k-F: attattcgaaggatccATGGCCACCATGCACCACCACCATCACCA, downstream primer ALDH2-pPIC3.5k-R: ccgccctagggaattcTTAGGAGTTCTTTTGTGGGACCTT), after obtaining the target fragment (including ALDH2 gene and His×6 tag), use homologous recombination enzyme ⅡOneStep Cloning Kit, the human acetaldehyde dehydrogenase ALDH2 gene and His×6 tag were constructed between the BamH I and EcoR I restriction sites of the pPIC3.5k plasmid to obtain the expression vector ALDH2-pPIC3.5k.

[0051] 2. Construction of yeast expression system. The plasmid ALDH2-pPIC3.5k obtained in step 1 wa...

Embodiment 3

[0054] Preparation 1 of embodiment 3 enzyme catalyzed hangover composition

[0055] (1) Alcohol dehydrogenase 1g, acetaldehyde dehydrogenase 1g, alcohol dehydrogenase agonist dithiothreitol 1g, acetaldehyde dehydrogenase agonist methionine 1g, and the common prosthetic group of the two dehydrogenases Mix 1 g of nicotinamide adenine dinucleotide evenly to obtain the enzymatic hangover hangover composition I.

[0056] (2) Constant volume: Dissolve the hangover agent composition I raw material obtained in step (1) in distilled water to prepare a 5 mg / mL aqueous solution.

[0057] (3) The aqueous solution in the step (2) is sterilized by filtration through a microporous membrane with a pore size of 0.22 μm, filled in a clean, dry and sterilized container, and sealed with a cover to obtain a hangover-relief composition.

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Abstract

The invention provides a composition with the enzymatic anti-inebriation effect and a preparation method and application thereof. The composition is prepared from 1-10 parts of alcohol dehydrogenase,1-10 parts of acetaldehyde dehydrogenase, 1-10 parts of an alcohol dehydrogenase agonist, 1-10 parts of an acetaldehyde dehydrogenase agonist and 0-10 parts of a common prothetic group of alcohol dehydrogenase and acetaldehyde dehydrogenase. The formula is comprehensive, the activity of an enzyme can be significantly improved, the antialcoholism effect is improved, and the agonists have the liverprotecting effect. The composition can be applied to preparation of an enzymatic antialcoholism agent, and drunkenness can effectively and rapidly relieved. Meanwhile, beneficial improvement on the dosage form and drug-delivery way of the composition is made, for example, by means of atomizing inhalation, the drugs can act on patients through the respiratory tract to achieve the effect of rapidlyrelieving the drunkenness, the use scenario is wider, and use is more convenient. The product is high in bioavailability, stable in quality, simple in production process, wide in application, convenient to use and easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of research and development of biological preparations, and in particular relates to an enzyme-catalyzed anti-alcoholism composition and its preparation method and application. Background technique [0002] my country's wine culture has a long history, the wine table culture is also extremely rich, and the wine market is huge. my country is the world's largest drinking country with a market of 800 billion. According to the latest data, China has surpassed Britain and the United States to become the world's largest alcohol consumption country. As big as the drinking market is, so is the sobering market. [0003] The metabolism of alcohol in the human body mainly depends on two enzymes: alcohol dehydrogenase and acetaldehyde dehydrogenase. Alcohol dehydrogenase can take off two hydrogen atoms in alcohol molecules, so that ethanol can be decomposed into acetaldehyde; and acetaldehyde dehydrogenase can take of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K9/12A61K9/72A61P39/02A61P1/16A61P13/12A23L33/00A61K31/095A61K31/4164A61K31/385A61K31/185A61K31/198A61K31/197A61K31/7084
CPCA61K38/443A61K38/44A61K31/095A61K31/4164A61K31/385A61K31/185A61K31/198A61K31/197A61K31/7084A61K9/0078A61K9/0019A61P39/02A61P1/16A61P13/12C12Y101/01001C12Y102/0101A23L33/00A23V2002/00A61K2300/00A23V2200/334
Inventor 江仁望刘康佳黄伟刘梦瑶刘洋
Owner JINAN UNIVERSITY