Lactobacillus plantarum capable of improving sleep and application thereof

A technology of Lactobacillus plantarum and strains, which is applied in the field of microbiology, can solve the problems that do not involve acid resistance, bile salt resistance and colonization ability of lactic acid bacteria, and achieve the effects of excellent simulated gastrointestinal tract survival ability, prolonged sleep time, and wide application prospects

Active Publication Date: 2019-08-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned patents do not involve the acid resistance, bile salt resistance and colonization ability of lactic acid bacteria, while the ability of probiotics to t

Method used

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  • Lactobacillus plantarum capable of improving sleep and application thereof
  • Lactobacillus plantarum capable of improving sleep and application thereof
  • Lactobacillus plantarum capable of improving sleep and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example 1 Screening of Lactobacillus plantarum

[0040] 1 Experimental method

[0041] Strain screening was performed with fresh human feces and kimchi water as samples.

[0042] (1) Pipette 0.5 mL of the sample into 5 mL of MRS medium and incubate at 37°C for 18-24 hours for enrichment.

[0043] (2) Gradient dilution: absorb 0.5 mL of the enriched sample in 4.5 mL of sterile normal saline to obtain 10 -1 Diluent, and then pipet 0.5mL 10 -1 The dilution is in 4.5 mL of saline to obtain 10 -2 Diluent, press this operation to get 10 -3 , 10 -4 , 10 -5 , 10 -6 Diluent.

[0044] (3) Plate-coated culture: draw 100μL of gradient dilution and spread it on the MRS plate, 10 -4 , 10 -5 , 10 -6 1 plate per gradient; incubate at 37°C for 48h.

[0045] (4) Streak separation: select colonies with typical characteristics on the coated plate according to the colony shape, size, edge, transparency, etc., pick the colonies with an inoculation loop and streak them on the selective medium plate, and...

Example Embodiment

[0056] Example 2 Research on the Survival Ability of Different Lactobacillus Plantarum Simulated Gastrointestinal Tract

[0057] 1 Test method

[0058] (1) The configuration of simulated gastric juice: Dissolve pepsin in sterilized physiological saline (0.9% w / v, adjusted to pH 3.0 with hydrochloric acid) to make the final concentration 3g / L. Filter with a 0.22μm sterile filter membrane, ready to use.

[0059] (2) The configuration of simulated intestinal juice: Dissolve trypsin in sterilized saline (0.9% w / v, pH adjusted to 8.0 with NaOH) to make the final concentration 1g / L, and add bile salt to make the final concentration 0.3 %. Filter with a 0.22μm sterile filter membrane, ready to use.

[0060] (3) The experiment was performed after three consecutive activations of different Lactobacillus plantarum (18h each time). Determine the strains that are continuously activated for three generations in A 600 Under the OD value, calculate the amount of bacterial solution required for OD...

Example Embodiment

[0069] Example 3 In vitro adhesion of different Lactobacillus plantarum to HT-29 cells

[0070] 1 Test method

[0071] The normal HT-29 cells were cultured and passaged, and different Lactobacillus plants were continuously activated for 3 generations. Collect the cultured cells, count them with a hemocytometer, and resuspend them to 2×10 in 1640 medium (with double antibodies and fetal bovine serum) 5 Pieces / mL. Add acid-washed and sterilized coverslips to the 6-well plate. Inoculate 2 mL of cell suspension, and wash 3 times with sterile PBS after the cells adhere to the cover glass (about 12h). Take a certain amount of bacteria liquid to collect the bacteria by centrifugation, wash the bacteria with sterile PBS once, then centrifuge again to collect the bacteria, and resuspend in 1640 medium (no double antibody, fetal bovine serum) to the bacteria concentration of 10 8 CFU / mL. Add 2mL 1640 culture medium bacterial suspension to each well of 6-well plate and incubate for 2h. Af...

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Abstract

The invention discloses lactobacillus plantarum capable of improving sleep and application thereof, and belongs to the technical field of microorganisms. The lactobacillus plantarum GDMCC No.60604 hasa good imitative gastrointestinal survival capability and a good cell adhesion capability and can prolong sleeping time induced by pentobarbital sodium and shorten the sleeping latent period inducedby pentobarbital sodium. The lactobacillus plantarum GDMCC No.60604 is used for preparing drug compositions for improving sleep and fermentation food and has extremely broad application prospects.

Description

technical field [0001] The invention relates to a plant lactobacillus capable of improving sleep and an application thereof, belonging to the technical field of microbes. Background technique [0002] Sleep is one of the important physiological processes of the body, and it has an important recovery function of eliminating physical fatigue and recovering body damage. But with the increasing stress of life or under the influence of various diseases, sleep disturbance has become a long-term problem for people. Studies have shown that about 50% of people in our country have experienced sleep disorders in varying degrees. Mild sleep disorders may lead to impaired memory, changes in concentration and learning difficulties, leading to a decline in one's own health and quality of life. Severe cases can cause pathological changes such as diabetes, cardiovascular disease, and cancer, or aggravate existing diseases. [0003] At present, the treatment of sleep disorders is mainly div...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/12A23C11/10A23L11/00A23L2/38A23L33/135A61K35/747A61P25/20C12R1/25A23L11/50
CPCA23C9/123A23C11/106A23L2/382A23L33/135A61K35/747A61P25/20A23V2002/00A23L11/50C12R2001/25C12N1/205A23V2400/169A23V2200/322
Inventor 陈卫田丰伟翟齐啸韩啸于雷雷赵建新张灏
Owner JIANGNAN UNIV
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