The invention discloses a processing method of an anti-mosquito polyester knitted fabric. The processing method comprises the following steps of 1 dyeing, wherein a gray fabric is put into a dye vat to be dyed, dispersing dye, a leveling agent DB-212 with the concentration of 1G/L, a deoiling agent DB-808 with the concentration of 1G/L and glacial acetic acid are added, the dyeing temperature is 130 DEG C, the bath ratio is 1:12, and the PH value is 5.5; 2 setting and drying, wherein the dyed fabric is taken out, cleaned in a clean water tank and then put into a setting and drying oven to be dried, the setting temperature ranges from 150 DEG C to 160 DEG C, and the setting time is 1 minute; 3 anti-mosquito padding finishing, wherein an anti-mosquito auxiliary solution is added in a rolling groove of a setting machine, the dried polyester knitted fabric passes through the padding solution contained in the rolling groove and then is delivered into the setting machine to be subjected to anti-mosquito finishing for 2 minutes, the polyester knitted fabric is taken out, and then the finished anti-mosquito polyester knitted fabric product is obtained. According to the processing method, the processing process is short, operation is easy, novel environment-friendly pyrethrin is low inconcentration and high in exhaustion rate, and the environment-friendly requirements are met.