Method for fabricating microneedle-based diagnostic skin patch coated with aptamer and patch

A technology of aptamers and microneedles, applied in biochemical equipment and methods, diagnosis, microneedles, etc., can solve problems such as little effect

Inactive Publication Date: 2019-08-09
(株)纳斯摩仕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-invasive methods such as iontophoresis, microdialysis, tape stripping, ultrasound, and microneedle have been used with little success (Paliwal et al., 2013 Diagnostic opportunities based on skinbiomarkers. European journal of pharmaceutical sciences: official journal of the European Federation for Pharmaceutical Sciences 50: 546-556)

Method used

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  • Method for fabricating microneedle-based diagnostic skin patch coated with aptamer and patch
  • Method for fabricating microneedle-based diagnostic skin patch coated with aptamer and patch
  • Method for fabricating microneedle-based diagnostic skin patch coated with aptamer and patch

Examples

Experimental program
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Embodiment 1

[0038] Example 1. Reaction Example of Adhering Aptamers to the Surface of Microneedles

[0039] In order to avoid the uncertainty of diagnosis caused by non-specific binding to the protein of the microneedle, after the surface of the microneedle was treated with polyethylene glycol (PEG), the aptamer was adhered to the polyethylene glycol. end, thereby making the device.

[0040] A. Examples of polydimethylsiloxane microneedles

[0041] Silanol (SiOH) is formed on the surface of polydimethylsiloxane constituting the microneedles by plasma oxidation. After silylation treatment of the hydroxyl group by 3-glycidyl etheroxypropyl trimethoxysilane with epoxy resin group, the epoxy resin group and the amino group of polyethylene glycol combined with the aptamer ( Figure 4 ) combined, so that the aptamer-polyethylene glycol ( image 3 ).

[0042] B. Method for coating the surface of polycarbonate microneedles with an aptamer bound to one end of polyethylene glycol

[0043] The ...

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Abstract

The present invention relates to a method for fabricating an aptamer-coated, microneedle-based diagnostic skin patch and a patch fabricated thereby. A patch of the present invention has the advantageof attaching a great number of aptamers, which are much smaller in size than antibodies, onto a relatively great number of microneedle tip surfaces. Allowing the attachment of aptamers for various kinds of biomarkers all together thereto, the patch can also simultaneously detect various kinds of materials (multiplexing). Therefore, a microneedle tip-based skin patch can also be used as a protein chip using an aptamer.

Description

technical field [0001] The present invention relates to a method for preparing a skin patch for diagnosis based on microneedles coated with an aptamer and the patch prepared by this method. Background technique [0002] Skin disease is a major health management problem in the world today. In addition to the more than one million new cases of skin cancer diagnosed each year in the United States (National Cancer Institute, www.cancer.gov), prediction and diagnosis of skin diseases are also important in their management. Current diagnostic methods rely primarily on visual observation and biopsy. However, detection methods that rely on visual observation are not necessarily effective in diagnosing skin conditions or diseases, and cannot detect risks or diseases until clinical signs appear. Furthermore, invasive methods such as biopsies not only increase trauma to the subject, but also increase the possibility of infection. Also, to be on the safe side, the method should be pe...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/543A61M37/00A61B5/00C12N15/115
CPCA61B5/441A61B5/685A61B2562/12A61M2037/0053A61B5/00C12N15/115C12N2310/16G01N33/54353A61M37/0015G01N33/5302G01N33/54393G01N2800/20A61B5/14514A61L31/06A61L31/088A61L31/16A61L2300/258A61L2420/02
Inventor 孙寅植
Owner (株)纳斯摩仕
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