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Establishment method of chicken inbred line

An inbred line and inbreeding coefficient technology, applied in the field of genetics, can solve the problems of ignoring the genetic background of matching individuals, incomplete pedigree records, and difficulty in accurately estimating the inbreeding coefficient of matching individuals, so as to avoid genetic background interference, The effect of reducing the impact of environmental factors and improving the success rate of formation

Pending Publication Date: 2019-08-16
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The general practice is to establish a family line, and then directly select individuals of full siblings (half siblings) for mating, but this method has many defects: first, it is difficult to accurately estimate the inbreeding coefficient of the selected individuals, even in the case of complete pedigrees, Because the establishment of any pedigree assumes that the inbreeding coefficient of the initial basic group individuals and the kinship coefficient between individuals are zero, and incomplete or even wrong pedigree records are common in production practice; background, it is difficult to operate flexibly according to specific inbreeding goals and required inbreeding rates
Third, without a scientific and rational selection and matching plan, it is impossible to avoid the serious decline in growth performance, reproductive performance, and viability of inbred offspring. In particular, reproductive performance is the first factor to consider when establishing inbred lines. relationship is difficult to sustain

Method used

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  • Establishment method of chicken inbred line
  • Establishment method of chicken inbred line
  • Establishment method of chicken inbred line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Taking Langshan chicken as an example, the method for forming the inbred line of Langshan chicken includes the following steps

[0057] (1) Formation of individual family members

[0058] The Langshan chicken flock at the peak egg production age of 28 to 35 weeks was used as the basic group, and 30 roosters and 300 hens with basically the same phenotypic characteristics such as black feathers, black shanks, erect single comb, and white skin were selected. Set up 30 individual families, each family consists of 1 rooster and 10 hens, and put them in individual cages.

[0059] (2) Family expansion and reproduction and record analysis

[0060] Perform artificial insemination on the hens in the established individual families, and mark the corresponding family and the corresponding hen number of each egg. For example, all the eggs produced by the first and partner hens of No. 1 family are recorded as 01-01. The breeding eggs produced by the 2 mating hens are all recorded a...

Embodiment 2

[0063] The formation of embodiment 2 chicken inbred lines

[0064] Step 1: Calculate the age at the start of laying, the weight of eggs at the beginning of laying, the weight of eggs at the beginning of laying, the number of eggs at 43 weeks, the weight of eggs at 43 weeks, and the weight at 43 weeks of all the family hens, and calculate the average , select healthy individuals with an upper and lower limit of 5-15% of the average value, and take into account the consistency of the phenotype,

[0065] Step 2: Verify the pedigree information of the healthy individuals selected in Step 1 and classify them into their families. Count the number of selected individuals in each family, and the families with the number of selected hens > 10 are used for the establishment of inbred lines;

[0066] Step 3: Collect 1.5ml of blood from the wing vein of the selected hen individual, extract the DNA and perform simplified genome sequencing to obtain the genomic single nucleotide polymorphi...

Embodiment 3

[0073] Embodiment 3 carries out the inbreeding coefficient test to the obtained chicken inbred line progeny

[0074] 1) Individual blood collection

[0075] Use a medical disposable syringe to collect 1.5ml of venous whole blood from the selected hen wings, and inject 1.5μL of 0.5mol / L Na immediately after collection 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for later use;

[0076] 2) Blood DNA extraction and quality testing

[0077] Aspirate 0.3ml of the spare blood stored in the enzyme-free tube at room temperature, use the phenol-chloroform extraction method from the peripheral blood of the animal to extract individual blood DNA, and analyze the integrity of the DNA by agarose gel electrophoresis, and detect the purity of the DNA with a spectrophotometer;

[0078] 3) Library construction and simplified genome sequencing

[0079] ①Extract 500ng of genomic DNA that has passed the quality inspection, add 0.6U EcoRI, T4 DNA ligas...

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Abstract

The invention discloses an establishment method of a chicken inbred line. According to the method, inbred line chickens with high inbreeding coefficient are obtained successfully by steps of family individual establishment, family expand reproduction and record analysis, chick feeding and management and establishment and forming of the chicken inbred line. The invention further discloses an identification method of the inbreeding coefficient of the chicken inbred line. By means of establishment of the chicken inbred line and identification of the inbreeding coefficient, the problems of inaccurate inbreeding coefficient and genetic relationship of individuals and low inbreeding coefficient under the condition of complete pedigree are solved; with the scientific and reasonable selecting, remaining and adapting scheme, the problem of severe recession of growth performance, reproductive performance, viability and the like of inbreeding descendants are effectively solved, and the problems that the inbred line is difficult to continue and the like are solved.

Description

technical field [0001] The invention relates to the field of genetics, in particular to a method for forming chicken inbred lines. Background technique [0002] Inbred strains refer to individuals whose phenotype tends to be stable and consistent through the inbreeding of continuous parents and offspring or half-siblings and full-siblings. Due to the relatively homozygous and stable phenotype and genome, inbred lines are used in the cultivation of new varieties (strains), the use of hybrid vigor and the study of the molecular mechanism of inbreeding depression, the identification of genes (chromosomal regions) related to important economic traits, and the elimination of harmful genes. It has important utilization value. The inbreeding degree of an inbred line is usually represented by the inbreeding coefficient, and the pedigree inbreeding coefficient is generally used. In recent years, with the development of molecular biology and bioinformatics technology, the molecular i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 韩威邹剑敏殷建玫朱云芬李国辉薛倩张会永苏一军王克华
Owner JIANGSU INST OF POULTRY SCI
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