Method for preparing high-purity lysophosphatidylcholine

A phosphatidylcholine, high-purity technology, applied in the field of preparation of phospholipid metabolites, can solve the problems of high comprehensive preparation cost, limited extraction efficiency, difficult to completely remove, etc., and achieve low comprehensive cost, high production efficiency and high yield high effect

Pending Publication Date: 2019-08-20
杨利平
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this practice has a serious dependence on raw materials. The raw material used is high-purity phosphatidylcholine (PC), which needs to be obtained through synthesis or complex extraction methods, which is expensive and not easy to obtain.
[0006] In the direction of preparing high-purity lysophosphatidylcholine from common soybean lecithin as the starting material, Japanese Patent JP63091306A discloses a preparation method using soybean lecithin as the raw material, after acetone deoiling, phospholipase A2 hydrolysis, silica gel column layer Analysis, ethanol-water elution, finally obtained lysophosphatidylcholine content is 87%, lysophosphatidylethanolamine content is 6% product; The technology described in this patented technology still has the limited purity of the product obtained, it is difficult to thoroughly remove other Problems with structurally similar components (such as lysophosphatidylethanolamine, etc.)
In addition, the patent CN104558021A discloses a method for preparing high-purity natural lysophosphatidylcholine from soybean or egg yolk phospholipids by direct column chromatography using polyhalogenated alkanes-low-carbon alcohol-water mixed eluent; However, limited by the extremely low content of lysophosphatidylcholine in the starting raw materials (for example, the total content of natural lysophosphatidylcholine in soybean lecithin is usually about 1%), the method described in this technology may have the possibility of product yield. Low yield, limited extraction efficiency and high comprehensive preparation cost

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  • Method for preparing high-purity lysophosphatidylcholine
  • Method for preparing high-purity lysophosphatidylcholine

Examples

Experimental program
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Effect test

Embodiment 1

[0044] In this example, 1.26 g of solid lysophosphatidylcholine with a purity of 99.6% was prepared from 20 grams of powdered soybean lecithin (with a phosphatidylcholine content of 23%) as raw material. specific:

[0045] (1) Add the powdered soybean lecithin and 38 grams of water into a 250 ml round-bottomed reaction bottle equipped with a Teflon mechanical stirring rod, and stir at 50° C. for 30 minutes to obtain an emulsion of soybean lecithin; 0.04 grams of phospholipase A1 Dissolve in 2 grams of water, then add dropwise into the emulsion of phospholipid and water within two minutes, keep warm at 50°C and continue stirring for 3 hours;

[0046] (2) Cool down to room temperature, add 200 ml of acetone under stirring and continue to stir at high speed for 2 hours; stop stirring and filter to separate the solid, which is dissolved in 60 ml of chloroform to obtain a solution; the solution is poured into 300 ml of methanol neutralize and stir vigorously; remove the formed sol...

Embodiment 2

[0049] In this example, 0.54 g of solid lysophosphatidylcholine with a purity of 99.1% was prepared from 20 grams of liquid soybean lecithin (with a phosphatidylcholine content of 11%) as a raw material. specific:

[0050] (1) Add the liquid soybean lecithin and 38 g of water into a 250 ml round-bottomed reaction bottle equipped with a Teflon mechanical stirring rod, and stir at 50°C for 30 minutes to obtain an emulsion; 0.04 g of phospholipase A1 is dissolved in 2 gram of water, and then dropwise added to the emulsion of phospholipid and water within two minutes, kept stirring at 50°C for 2 hours;

[0051] (2) Cool down to room temperature, add 200 ml of acetone under stirring and continue to stir at high speed for 2 hours; stop stirring and filter to separate the solid, which is dissolved in 60 ml of dichloroethane to obtain a solution; the solution is poured into 300 ml in isopropanol and vigorously stirred; the formed solid insolubles were removed by filtration, the filtr...

Embodiment 3

[0054] In this example, 1.22 grams of solid lysophosphatidylcholine with a purity of 99.3% was prepared from 20 grams of powdered soybean lecithin (with a phosphatidylcholine content of 23%) as raw material. specific:

[0055] (1) Add the powdered soybean lecithin and 58 g of water to a 500 ml round-bottomed reaction bottle equipped with a Teflon mechanical stirring rod, and stir at 50° C. for 30 minutes to obtain an emulsion; 0.03 g of phospholipase A1 is dissolved in 2 gram of water, and then added dropwise to the emulsion of phospholipids and water within two minutes, kept stirring at 50°C and continued to react for 4 hours;

[0056] (2) Cool down to room temperature, add 300 ml of acetone under stirring and continue to stir at high speed for 2 hours; stop stirring and filter to separate the solid, which is dissolved in 60 ml of dichloromethane to obtain a solution; the solution is poured into 300 ml of ethanol neutralize and stir vigorously; remove the formed solid insolu...

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Abstract

The invention discloses a method for preparing high-purity lysophosphatidylcholine. The method comprises the steps of enabling soybean phospholipid to dissolve in water or a calcium chloride solution,adding phospholipase, and performing an enzymolysis reaction; pretreating enzymolysis reaction products, conveying the pretreated enzymolysis reaction products into a silica gel column, performing repeated eluting by using multi-halogeno low-carbon alkane-low-carbon alcohol-water as an eluant, collecting a solution of an object, and performing concentration and drying so as to obtain high-puritylysophosphatidylcholine products. According to the method disclosed by the invention, ordinary soybean phospholipid is used as a raw material, through the enzymolysis reaction, pretreatment after enzymolysis and column chromatography and refiningtechnology, and the high-purity lysophosphatidylcholine is prepared; and the high-purity lysophosphatidylcholine has the characteristics that the raw materials are cheap and easy to obtain, the technology is simple, the maneuverability is high and product yield is high, is suitable for industrialized production, and the produced lysophosphatidylcholineis high in purity, few in by-products and stable in product quality.

Description

technical field [0001] The invention relates to a method for preparing phospholipid metabolites, in particular to a method for preparing high-purity lysophosphatidylcholine. Background technique [0002] Lysophosphatidylcholine (LPC for short) is one of the metabolites of phospholipids, also known as lysophosphatidylcholine, which is a product formed after phosphatidylcholine removes a fatty acid chain. The emulsification ability and emulsification stability of lysophosphatidylcholine are superior to ordinary phospholipid products; in addition, lysophosphatidylcholine also has unique physiological activity, antibacterial activity and antioxidant properties. Therefore, lysophosphatidylcholine can be used as an additive, pharmaceutical intermediate, drug carrier and high-efficiency emulsifier in the food industry, pharmaceutical industry, cosmetics industry and feed industry. [0003] For example, in the field of medicine, high-purity lysophosphatidylcholine is one of the key...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
CPCC12P13/00
Inventor 杨利平陈鑫
Owner 杨利平
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