Method for determining virulence of bactericide to wheat stripe rust

A technology of wheat stripe rust and fungicide, which is applied in the direction of testing pharmaceutical preparations, image data processing, instruments, etc., can solve the problems of low repeatability and large human factors in the measurement method, and achieve less dosage, stable results, accurate results

Inactive Publication Date: 2019-08-20
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are affected by many factors during the test, including the formulation and dose of the drug, the growth of the crop, etc., the repeatability is not high, and the measurement method of the data is greatly affected by human factors, and the workload and plant materials need to reach a certain level. The amount to adjust the rationality of the data

Method used

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  • Method for determining virulence of bactericide to wheat stripe rust
  • Method for determining virulence of bactericide to wheat stripe rust
  • Method for determining virulence of bactericide to wheat stripe rust

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 prepares test petri dish

[0055] 1. Isolation and propagation of wheat stripe rust strains

[0056] (1) Wheat planting: First, select plump and consistent Mingxian 169 wheat seeds, soak them in a 500mL beaker with clean water for one night, after the seeds fully absorb water and swell, pour them into a petri dish with a diameter of 25cm, dry the surface water, and cover Wet gauze, put it into a black plastic bag, and put it in a constant temperature box at 25°C to accelerate germination; secondly, after the seeds are white, mix the peat soil and vermiculite, add water to moisten it, and spread it in a small black 7-square plastic flowerpot. Select healthy Lubai wheat seeds and sprinkle them in the middle, put 15-20 seeds in one pot, cover with a layer of culture soil, water thoroughly, and place in the greenhouse (16h / 8h light / dark), wait for 10d to grow to 1 core Use 1 leaf for later use.

[0057] (2) Collection of wheat stripe rust: first collect disea...

Embodiment 2

[0091] The application test of embodiment 2 detached leaf section plate containing medicine

[0092] In conjunction with the preferred result of above-mentioned embodiment 1, randomly select wheat stripe rust single spore heap isolated bacterial strain, adopt plate-containing drug method to carry out the toxicity determination of triadimefon to wheat stripe rust, concrete method is as follows:

[0093] (1) Preparation of spore suspension: Take out the isolated strains of single spore piles stored at -80°C, place them in a water bath at 42°C for 5 minutes to obtain spore powder, pour an appropriate amount of spore powder into a 10mL centrifuge tube, and add 1mL of 0.1 % Tween 20, shake on a vortex shaker for 1 minute, add 9 mL of water, shake again for about 4 minutes, until the spore powder is fully dispersed to make a spore suspension.

[0094] (2) Inoculation: Spray 75% alcohol to the operating table earlier, then spray a small amount of clear water, and leave it for 2 minut...

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Abstract

The invention relates to a method for determining virulence of bactericide to wheat stripe rust and belongs to the physical protection field. An inhibition condition of triadimefon to the virulence ofthe wheat stripe rust is determined through adopting steps of spraying inoculation of a stripe rust uredospore suspension liquid, fresh-keeping culture medium preparing, in-vitro leaf segment medicament treating, detection and the like. In the invention, an in-vitro leaf segment mode is adopted to replace a whole plant to carry out testing, which is beneficial to solving problems of a large amount of test samples and large influence factors in a test process. Effects of different preservatives and a fresh-keeping substrate on spore germination, and the effects of medicine spraying, dipping and containing modes on an inhibition rate of the stripe rust after bactericide treatment is performed on the in-vitro leaf segment are also determined so as to determine an optimal scheme. The method has high repeatability and potential socioeconomic significance.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a method for measuring the toxicity of fungicides to wheat stripe rust. Background technique [0002] Rust is an important disease that harms wheat production, and its pathogenic fungus Rust is one of the ten hot research fungi. Its occurrence and harm are long-term, explosive, epidemic and variable, and its effective prevention and control has become a long-term problem. At present, different measures such as agricultural methods, breeding of disease-resistant varieties, chemical control, and biological control are usually used in actual production to control and reduce the harm. Among various control measures, chemical control is one of the main methods for the control of wheat stripe rust. [0003] Many research institutes and enterprises at home and abroad have been researching, innovating and screening new fungicides that have significant control effects on whea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15G06T7/62
CPCG01N33/15G06T7/62
Inventor 毕朝位彭复蓉杨宇衡余洋
Owner SOUTHWEST UNIVERSITY
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