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Nano-silver compound modified by S-layer protein and preparation method and application of nano-sliver compound

A protein-modified and nano-silver technology is applied in the field of S-layer protein-modified nano-silver composites and their preparation to achieve the effects of improving biocompatibility, good antibacterial effect, and strong agglutination-promoting effect

Active Publication Date: 2019-08-23
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But these functions are only part of the surface protein's antibacterial mechanism

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  • Nano-silver compound modified by S-layer protein and preparation method and application of nano-sliver compound
  • Nano-silver compound modified by S-layer protein and preparation method and application of nano-sliver compound
  • Nano-silver compound modified by S-layer protein and preparation method and application of nano-sliver compound

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Effect test

Embodiment 1

[0033] The preparation method of the composite (SLP-AgNPs) of S-layer protein modified nano-silver of the present invention is as follows:

[0034] Lactobacillus buchneri (Lactobacillus buchneri) CICC20023 (China Industrial Microbiology Culture Collection and Management Center) was used to culture the S-layer protein to the logarithmic phase of growth in liquid MRS medium, and the bacterial sediment was collected by centrifugation; add 10 mL of wet bacterial cells per gram LiCl solution (5mol / L) was added to the incubator at 37°C for 0.5h, and the supernatant was discarded by centrifugation; then 5mL of CuHCl solution (4mol / L) was added, placed at 37°C for 1h, and the supernatant was collected by centrifugation again; The supernatant was poured into a 14kDa dialysis bag, dialyzed with ultrapure water at 4°C for 48 hours, and finally the precipitate was collected by centrifugation to obtain the surface protein. The precipitate was dissolved in 8 mol / L urea-Tris-HCl solution, th...

Embodiment 2

[0037] The preparation method of the composite (SLP-AgNPs) of S-layer protein modified nano-silver of the present invention is as follows:

[0038] Lactobacillus buchneri (Lactobacillus buchneri) CICC20023 (China Industrial Microbiology Culture Collection and Management Center) was used to culture the S-layer protein to the logarithmic phase of growth in liquid MRS medium, and the bacterial sediment was collected by centrifugation; add 10 mL of wet bacterial cells per gram LiCl solution (5mol / L) was added to the incubator at 37°C for 0.5h, and the supernatant was discarded by centrifugation; then 5mL of CuHCl solution (4mol / L) was added, placed at 37°C for 1h, and the supernatant was collected by centrifugation again; The supernatant was poured into a 14kDa dialysis bag, dialyzed with ultrapure water at 4°C for 48 hours, and finally the precipitate was collected by centrifugation to obtain the surface protein. The precipitate was dissolved in 8 mol / L urea-Tris-HCl solution, th...

Embodiment 3

[0041]The preparation method of the composite (SLP-AgNPs) of S-layer protein modified nano-silver of the present invention is as follows:

[0042] Lactobacillus buchneri (Lactobacillus buchneri) CICC20023 (China Industrial Microbiology Culture Collection and Management Center) was used to culture the S-layer protein to the logarithmic phase of growth in liquid MRS medium, and the bacterial sediment was collected by centrifugation; add 10 mL of wet bacterial cells per gram LiCl solution (5mol / L) was added to the incubator at 37°C for 0.5h, and the supernatant was discarded by centrifugation; then 5mL of CuHCl solution (4mol / L) was added, placed at 37°C for 1h, and the supernatant was collected by centrifugation again; The supernatant was poured into a 14kDa dialysis bag, dialyzed with ultrapure water at 4°C for 48 hours, and finally the precipitate was collected by centrifugation to obtain the surface protein. The precipitate was dissolved in 8 mol / L urea-Tris-HCl solution, the...

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Abstract

The invention discloses a nano-silver compound modified by S-layer protein and a preparation method and bacterial inhibition application of the nano-sliver compound. The purified S-layer protein is extracted from lactic acid bacteria, and under the electrostatic interaction, the S-layer protein is self-assembled on the surface of nano-sliver to form the nano-silver compound. According to the nano-silver compound and the preparation method, the good adhesiveness and bacterial inhibition activity of the S-layer protein are utilized for modifying the nano-sliver, so that the nano-sliver has higher bacterial inhibition performance, the foundation is laid for developing a safe and effective food-grade antibacterial agent, the nano-sliver achieves the high bacterial inhibition effect, through combination of the S-layer protein and the nano-sliver, a synergetic antibacterial effect can be triggered, the problem of the drug tolerance, caused by antibiotic abuse, of bacterial strains can be solved, and the application range of the nanotechnology in the fields of food, drugs and the like is enlarged.

Description

technical field [0001] The invention belongs to the technical field of nano-silver composites, and in particular relates to a nano-silver composite modified by S-layer protein and its preparation method and application. Background technique [0002] At present, the application of antibiotics is relatively common, which also makes bacteria show some drug resistance, and even some superbugs have appeared. Superbugs such as the multidrug-resistant Gram-negative Klebsiella pneumoniae, Escherichia coli and the Gram-positive methicillin-resistant Staphylococcus aureus are responsible for hundreds of thousands of deaths and many Big medical bills. Everyone is also looking forward to finding new ways to replace antibiotics for antibacterial. In recent years, nanotechnology has been widely used in the food field, and a variety of nano-antibacterial particles have been used as food antibacterial agents. [0003] At present, scholars have reported that the surface protein of lactic ...

Claims

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Application Information

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IPC IPC(8): A61K33/38A61K47/64A61K38/16A61P31/04
CPCA61K33/38A61K38/164A61K47/64A61P31/04A61K2300/00
Inventor 饶胜其陈瑞焦新安伍能建杨振泉胡钦郑香峰高璐
Owner YANGZHOU UNIV
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