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Application of purple sweet potato polysaccharides in preparation of lung cancer resistant drugs

An anti-lung cancer drug, the technology of purple sweet potato, applied in the field of medicine, can solve the problem of less research on anti-tumor activity, and achieve the effect of broadening the application field and low cost

Active Publication Date: 2019-08-23
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition to various nutrients, the stems, leaves and roots of purple sweet potato are also rich in anthocyanins and polysaccharides. The aqueous extract of purple sweet potato has anti-mutation and anti-oxidation capabilities, and the pharmacological effects of polysaccharides are currently a research hotspot. , but mainly focused on the research on antioxidant activity, and the research on the antitumor activity of purple sweet potato polysaccharides is less

Method used

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  • Application of purple sweet potato polysaccharides in preparation of lung cancer resistant drugs
  • Application of purple sweet potato polysaccharides in preparation of lung cancer resistant drugs
  • Application of purple sweet potato polysaccharides in preparation of lung cancer resistant drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Extraction and purification of the crude polysaccharide of purple sweet potato of embodiment 1

[0022] The invention uses an ultrasonic extraction method to extract polysaccharides from purple sweet potatoes, and optimizes the extraction process by using response surface analysis.

[0023] 1. Crude polysaccharide extraction

[0024] Extraction process: purple sweet potato Ningzi No. 4→washing→drying at 40°C→crushing→passing through an 80-mesh sieve→adding ultrapure water→extracting with ultrasonic extraction equipment→filtering→concentrating with a rotary evaporator→ethanol precipitation→Sevage deproteinization→filtering→ Calculate the crude polysaccharide yield.

[0025] (1) Ultrasonic extraction of polysaccharides, specifically as follows: the purple sweet potato Ningzi No. 4 sample was washed, cut into pieces, dried, pulverized, and passed through a 80-mesh sieve to obtain purple sweet potato powder;

[0026] (2) Soak the purple sweet potato powder with 95% ethano...

Embodiment 2

[0042] Example 2 FsPSP-1 inhibits proliferation of human lung cancer cells

[0043] In order to study the effect of FsPSP-1 on the growth and proliferation of human lung cancer cells, the inhibitory effect of FsPSP-1 extracted in Example 1 on human lung cancer cells (A549, H460, H1299) (purchased from Shanghai Cell Bank, Chinese Academy of Sciences) was tested by MTT method . The specific operation is as follows:

[0044] 1. Inoculate the thawed and recovered tumor cell lines A549, H460 and H1299 in 1640 medium containing 10% newborn bovine serum respectively, and place them at 37°C and 5% CO 2 , cultured in an incubator with saturated humidity;

[0045] 2. Take the tumor cells to be tested in the logarithmic growth phase to make 1×10 4 / mL single cell suspension, seeded in 96-well plate, 200uL per well, placed at 37°C, 5% CO 2 Cultivate under conditions for 24 hours until the cells adhere to the wall;

[0046] 3. Discard the original culture medium, add different concent...

Embodiment 3

[0052] Example 3 Effect of FsPSP-1 on Colony Formation of Lung Cancer Cells

[0053] Using the purple sweet potato polysaccharide FsPSP-1 extracted in Example 1, a colony formation experiment was performed to detect the long-term inhibitory effect of FsPSP-1 on human lung cancer cells (A549, H460). The specific operation is as follows:

[0054] 1. Take the A549 and H460 cells in the logarithmic growth phase and inoculate them in 24-well plates respectively, and the cell inoculation density is 300 cells / well;

[0055] 2. 37°C, CO 2 Cultivate in an incubator. After the wall is completely attached, the original culture solution is discarded, and different concentrations of FsPSP-1 are added according to the group. The set polysaccharide concentration is: 0ug / ml, 5ug / ml, 10ug / ml, 15ug / ml ml, 200ug / ml, 30ug / ml, the medium is changed every 3 days;

[0056] 3. After 14 days, there are cell colonies visible to the naked eye, and a cell mass consisting of more than 50 cells is count...

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Abstract

The invention discloses application of purple sweet potato polysaccharides in preparation of lung cancer resistant drugs. The purple sweet potato polysaccharides are heteropolysaccharides extracted from Ningzi #4 purple sweet potatoes, and are mainly composed of three kinds of monosaccharides of xylose, rhamnose and glucose, wherein the xylose content is 84.92+ / -1.57%, the rhamnose content is 2.15%, the glucose content is 0.57%, and the average molecular weight is 0.82*10<4>Da. The purple sweet potato polysaccharides have effects of obviously inhibiting cell proliferation of lung cancer cellssuch as A549, H1299 and H460, the BALB / c mouse subcutaneous tumor volume subjected to purple sweet potato polysaccharide FsPSP-1 treatment is obviously reduced, and the tumor weight is obviously reduced. The invention provides novel application of the purple sweet potato polysaccharides in preparation of lung cancer resistant drugs, the application field of the purple sweet potato polysaccharidesis broadened, a material basis is provided for developing natural activity antitumor drugs, and the application has potential social benefits and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to the application of sweet potato polysaccharide, in particular to the application of purple sweet potato polysaccharide in the preparation of anti-lung cancer drugs. Background technique [0002] In recent years, the global incidence of lung cancer has increased by 10-30 times for men and 3-8 times for women. Lung cancer presents the characteristics of "three highs and one low", that is, high incidence, high mortality, high growth rate, and low cure rate. Surgery is the most effective treatment for patients with early-stage lung cancer, but most patients are diagnosed at an advanced stage. Chemotherapy is the main treatment for metastatic lung cancer. However, even with the advances in molecular targeted therapy for lung cancer today, the overall 5-year survival rate of lung cancer is still lower than 20%. [0003] The treatment of lung cancer is still dominated by platinum-based ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61K36/39
CPCA61K31/715A61K36/39A61P35/00C08B37/0003
Inventor 李荣鹏布会敏袁博蒋继宏黄晓梅朱鹏程蒋杰邦吴叶娇
Owner XUZHOU NORMAL UNIVERSITY
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