Hydrogel containing NP10 antimicrobial peptide as well as preparation method and application thereof
A hydrogel and antibacterial peptide technology, applied in the field of hydrogel containing NP10 antibacterial peptide and its preparation, can solve the problems of unsatisfactory antibacterial performance, shortening wound healing time, etc.
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[0030] The invention provides the preparation method of described hydrogel, comprises the following steps:
[0031]1) Mix and swell the gel matrix and water to obtain No. 1 solution;
[0032] 2) mixing the No. 1 solution with sodium β-glycerophosphate to obtain the No. 2 solution;
[0033] 3) dissolving the NP10 antimicrobial peptide in water to obtain an NP10 antimicrobial peptide solution;
[0034] 4) the No. 2 solution, CaCl 2 The aqueous solution and the NP10 antimicrobial peptide solution are mixed to obtain a hydrogel;
[0035] There is no time sequence limitation between the steps 1)-2) and step 3).
[0036] In the present invention, the flow process of the preparation method is shown in figure 1 . When the gel matrix preferably includes sodium alginate and carboxymethyl chitosan, mix and stir sodium alginate and water, and swell for 12 to 24 hours to obtain liquid A; mix and dissolve carboxymethyl chitosan and water, and ultrasonicate for 13 hours ~20min to obtai...
Embodiment 1
[0041] Prepare the following raw materials in mass percentages: NP10 antimicrobial peptide 0.03%, β-sodium glycerophosphate (β-GP) 2%, sodium alginate 3%, carboxymethyl chitosan 2%, 1% CaCl 2 6% aqueous solution, distilled water to make up the balance.
[0042] Containing the preparation method of NP10 antimicrobial peptide hydrogel, comprises the following steps:
[0043] a. Precisely weigh a certain amount of sodium alginate at room temperature and evenly sprinkle it in 600ml distilled water, stir and swell for 12-24 hours to obtain liquid A;
[0044] b. Precisely weigh a certain amount of carboxymethyl chitosan and dissolve it in 200ml distilled water, stir evenly, ultrasonicate for 15 minutes to fully dissolve it, and place it at room temperature to obtain liquid C;
[0045] c. Add a certain amount of β-GP to liquid A to obtain liquid B;
[0046] d. Add 0.1g CaCl 2 Dissolve in 10ml distilled water to get 1% CaCl 2 solution to obtain liquid D;
[0047] e. Dissolve a ce...
Embodiment 2
[0050] Prepare the raw materials of the following mass percentages: NP10 antimicrobial peptide 0.045%, β-sodium glycerophosphate (β-GP) 1%, sodium carboxymethylcellulose 5%, 1% CaCl 2 7% aqueous solution, distilled water to make up the balance.
[0051] Containing the preparation method of NP10 antimicrobial peptide hydrogel, comprises the following steps:
[0052] a. Precisely weigh a certain amount of sodium carboxymethylcellulose at room temperature and evenly sprinkle it in 600ml of distilled water, stir and swell for 12-24 hours to obtain liquid A;
[0053] b. Add a certain amount of β-GP to liquid A to obtain liquid B;
[0054] c. Add 0.1g CaCl 2 Dissolve in 10ml distilled water to get 1% CaCl 2 solution to obtain liquid D;
[0055] d. Dissolve a certain amount of antimicrobial peptide powder in 10ml distilled water with precision weighing at room temperature to obtain F;
[0056] e. Take an appropriate amount of liquid E, liquid F and liquid D, mix, stir evenly to ...
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