Method for determining free vitamin D in sample through liquid chromatography tandem mass spectrometry

A liquid chromatography and tandem mass spectrometry technology, which is applied in the field of liquid chromatography tandem mass spectrometry for the determination of free vitamin D in samples, can solve the problems of no liquid chromatography tandem mass spectrometry detection of Free-25(OH)D, no supporting kits, etc. , to increase the detection specificity, ensure the steady state, and avoid the effect of interference
CN110174477AActive Publication Date: 2019-08-27北京豪思生物科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京豪思生物科技股份有限公司
Publication Date
2019-08-27

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Abstract

The present invention provides a method for determining free vitamin D in a sample through liquid chromatography tandem mass spectrometry. The method comprises: preparing free vitamin D standard substance solutions of different concentrations; adding stable isotope internal standard substances to the standard substance solutions, uniformly mixing, then extracting with n-hexane, drying supernatantswith nitrogen, adding PTAD for derivatization and then drying with the nitrogen, and adding a complex solution for detection of liquid chromatography tandem mass spectrometry; drawing a standard curve according to the concentrations of the standard substance solutions and the detected signal intensity; replacing the standard substance solution in step B with a sample to be determined for conducting detection; and obtaining the concentration of the free vitamin D in the sample to be determined according to the detection result of the sample to be determined with reference to the standard curve, thereby realizing quantitative detection of the free vitamin D in the sample. The present invention successfully establishes the method for determining the free vitamin D in the sample through liquid chromatography tandem mass spectrometry. The method can quickly and specifically detect the content of the free vitamin D in the sample (especially serum).
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Description

technical field

[0001] The invention relates to liquid chromatography tandem mass spectrometry detection technology, in particular to a liquid chromatography tandem mass spectrometry method for determining free vitamin D in a sample. Background technique

[0002] Vitamin D is a fat-soluble vitamin necessary to maintain human health. As a hormone, it regulates calcium and phosphorus metabolism and promotes healthy growth and reconstruction of bones. There are two main forms of vitamin D: ergocalciferol (vitamin D2) and cholecalciferol (vitamin D3). Vitamin D cannot be synthesized by itself, and can only be synthesized by sunlight or external sources of vitamin D2 or D3. Vitamin D enters the liver through blood circulation and is transformed into 25(OH)D, which is then metabolized into dihydroxyvitamin D [1,25(OH)2D] by the renal 1α hydroxylase CYP27B1, which then acts on vitamin D receptors , play a biological effect. In addition to directly causing rickets and osteoporosis...

Claims

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