Method for batch preparation of triglyceride oil base with different chain lengths by purifying vegetable oil
A vegetable oil and triglyceride technology, applied in chemical instruments and methods, separation methods, fat oil/fat refining, etc., can solve problems affecting vegetable oil composition and fatty acid distribution, and achieve the effect of increasing yield and reducing the number of purifications
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Embodiment 1
[0042] The inventive method step is as follows (taking rice oil as example):
[0043] 1. Pretreatment of chromatographic column adsorbent: activate pretreatment of the adsorbent, the adsorbent includes alumina, silicic acid, activated carbon, silica gel and, among them, alumina: fully washed, heated at 200°C for 4h ;Silicic acid: fully washed, filtered, activated at 110°C for 20h; Activated carbon: fully washed, soaked in 2 drops of 2mol / L dilute hydrochloric acid, rinsed with distilled water several times until the pH test paper shows neutral, baked at 105°C for 5h ; Silica gel: activated at 107°C for 1.5h;
[0044]2. Filling of chromatographic column adsorbent: Use n-hexane to evenly fill the adsorbent alumina, silicic acid, activated carbon, and silica gel into the chromatographic column at a height ratio of 2:1:1:2, and fill the adsorbent to the height of 30cm, in order to prevent the packing from leaking, the bottom layer is covered with a layer of absorbent cotton;
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Embodiment 2
[0050] Test Example 1 prepared long-chain triglyceride oil base based on chromatographic column purification of rice oil and determined the impact on the composition of rice oil after purification, including the following detection:
[0051] (1) Effect of chromatography column purification on oryzanol in rice oil:
[0052] Adopt the reverse phase high performance liquid chromatography (C18 post) to measure the content of oryzanol, the result is as follows figure 2 As shown in (a), no oryzanol was detected in the oil base after one purification, indicating that the method for preparing rice oil oil base based on chromatographic purification in the present invention can completely remove oryzanol in rice oil, and the removal rate is basically 100% .
[0053] (2) Effect of chromatography column purification on tocopherol in rice oil:
[0054] Adopt normal phase high performance liquid chromatography (Sehperisorb Silica column) to measure tocopherol composition and content, the...
Embodiment 3
[0064] The difference between this embodiment and Example 1 is that the adsorbent is silicic acid, silica gel, activated carbon, alumina, magnesium silicate, diatomaceous earth, kaolin, silicic acid: silica gel: activated carbon: alumina: magnesium silicate: diatom Soil: kaolin is 2: 4.5: 4: 5: 1: 1: 4, the height of filling adsorbent is 30cm, and other conditions are the same as in Example 1,
[0065] Chromatographic column effect diagram (such as Figure 4 ), the test rice oil yield was 81.38%. The reason may be that after all the adsorbents are mixed, the activated carbon with less density is distributed in the whole prepared chromatographic column, and the shape of diatomite is fluffy, fine particles, uncertain properties and viscous soft, difficult to filter, so that despite using the same proportion of n-hexane as in Example 1, a large amount of oil remains in the chromatographic column; detect the content of tocopherol and oryzanol in the rice oil after purification, th...
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