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Oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and preparation method and application thereof

A particle complex, oyster polypeptide technology, applied in application, food science, function of food ingredients, etc., can solve the problems of nano selenium size regulation stability and active molecule enrichment efficiency, achieve good hangover and liver protection, improve stability Sexual, effective effect

Active Publication Date: 2019-09-06
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nano-selenium preparation technology and synergistic incorporation of active molecules are widely used in the research and development of related innovative medicines. At present, there are mainly problems such as nano-selenium size control, stability, and active molecule enrichment efficiency.

Method used

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  • Oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and preparation method and application thereof
  • Oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and preparation method and application thereof
  • Oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] A preparation method of an oyster polypeptide-nano selenium particle complex having the effect of relieving alcohol and protecting the liver, comprising the following steps:

[0045] (1) Using commercially available oysters as raw materials, remove the shells and take the meat, wash the meat and homogenate directly (the homogenization speed is 12000 r / min, and the homogenization time is 60 s) to form a homogenate liquid, and add water (homogenization time is 60 s). The mass ratio of slurry to water is 1:1), pancreatin is added for enzymolysis, the enzymolysis conditions are: pH 7.0, the enzyme addition amount is 0.1% (w / w) (that is, the enzyme addition amount is 0.1% of the mass of oyster meat %), the reaction temperature was 60 °C, and the reaction time was 4 h. After the enzymatic hydrolysis reaction, inactivate the enzyme in a boiling water bath for 10 min, centrifuge at 8000 r / min at 4 °C for 30 min, and freeze-dry the obtained supernatant (the pre-freezing temperat...

Embodiment 2

[0048] A preparation method of an oyster polypeptide-nano selenium particle complex having the effect of relieving alcohol and protecting the liver, comprising the following steps:

[0049] (1) Using commercially available oysters as raw materials, remove the shells and take the meat, wash the meat and homogenate directly (the homogenization speed is 12000 r / min, and the homogenization time is 60 s) to form a homogenate liquid, and add water (homogenization time is 60 s). The mass ratio of slurry to water is 1:1), adding trypsin Papain for enzymolysis, the conditions of enzymolysis are: pH 5, the enzyme addition amount is 1% (w / w) (that is, the enzyme addition amount is 1% of the mass of oyster meat %), the reaction temperature was 37°C, and the reaction time was 12 h. After the enzymatic hydrolysis reaction, inactivate the enzyme in a boiling water bath for 15 minutes, centrifuge at 10,000 r / min for 10 minutes at 10°C, and freeze-dry the obtained supernatant (the pre-freezing...

Embodiment 3

[0052] A preparation method of an oyster polypeptide-nano selenium particle complex having the effect of relieving alcohol and protecting the liver, comprising the following steps:

[0053] (1) Using commercially available oysters as raw materials, remove the shells and take the meat, wash the meat and homogenate directly (the homogenization speed is 12000 r / min, and the homogenization time is 60 s) to form a homogenate liquid, and add water (homogenization time is 60 s). The mass ratio of slurry to water is 1:2), adding 2.4 L of alkaline protease Alcalase for enzymolysis, the enzymolysis conditions are: pH 11, the enzyme addition is 0.4% (w / w) (that is, the enzyme addition is oyster 0.4% of the meat mass), the reaction temperature was 40°C, and the reaction time was 0.5 h. After the enzymatic hydrolysis reaction, inactivate the enzyme in a boiling water bath for 10 min, centrifuge at 10,000 r / min at 4 °C for 15 min, and freeze-dry the obtained supernatant (the pre-freezing te...

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Abstract

The invention discloses an oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and a preparation method and application thereof. The method includes: homogenizing oyster meat, adding water and active enzyme, enzymolyzing, allowing boiling water bath, centrifuging to take supernate, cold-drying the supernate to obtain oyster polypeptide powder, adding the oyster polypeptide powder into water, well mixing to obtain a solution, adding a sodium arsenite solution into the oyster polysaccharide solution, well mixing, adding an ascorbic acid solution while stirring, allowing water bath reaction, dialyzing to obtain functionalized nano selenium sol, ultrasonically treating, and freeze-drying to obtain the oyster polypeptide-nano selenium granulecompound. Oyster polypeptide is used to stabilize nano selenium, so that stability of nano selenium is remarkably improved, and nano selenium can be stably stored at 4 DEG C for one month; through MTT method detection, nano granules can relieve damage effect of alcohol or hydrogen peroxide on liver. If the compound is converted into healthcare products having a liver protecting function and capable of supplementing selenium element, great social and economic benefit can be generated.

Description

technical field [0001] The invention belongs to the technical field of nano-selenium nutritional preparations and functional food preparations, and in particular relates to an oyster polypeptide-nano-selenium particle compound with the effect of relieving alcohol and protecting the liver, as well as its preparation method and application. Background technique [0002] Selenium (Selenium, Se) is one of the essential and indispensable nutritional elements in organisms, and is an important component of human erythrocyte glutathione peroxidase. Its main function is to participate in the synthesis of enzymes and avoid excessive oxidation of cell membranes. , protect its structure and function, and has multiple biological functions such as anti-oxidation, anti-tumor, prevention of cardiovascular disease, and epilepsy. [0003] Nano-selenium belongs to zero-valent selenium. The general zero-valent element selenium cannot be absorbed and utilized by the human body, but nano-selenium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/16A23L33/18A23L17/00A23L5/30
CPCA23L33/16A23L33/18A23L17/65A23L5/32A23V2002/00A23V2200/30A23V2200/32A23V2250/1626A23V2250/55A23V2300/48Y02A50/30
Inventor 赵谋明周非白郑泽洋苏国万刘小玲
Owner SOUTH CHINA UNIV OF TECH
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