Composition, preparation method and application thereof in killing cell strain

A composition-receptor binding technology, applied in the field of composition, application of killing cell lines, and preparation, can solve the problems of difficulty in taking into account in vivo stability, target aggregation ability and poor carrier degradation performance, etc., to improve tumor Targeted delivery effect, promotion of sonodynamic effect, effect of high biosafety

Active Publication Date: 2019-09-06
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of this, the present invention provides a composition, a preparation method and its application in killing cell lines, which are used to solve the pr

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  • Composition, preparation method and application thereof in killing cell strain
  • Composition, preparation method and application thereof in killing cell strain
  • Composition, preparation method and application thereof in killing cell strain

Examples

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Example Embodiment

[0039] Example 1

[0040] This example is one of the examples for preparing BP-PEG-T7 / 5-ALA NSs.

[0041] Step 1. Preparation of BP NSs: Black scales are prepared by liquid phase stripping method to prepare BP NSs; among them, the liquid phase stripping method is: black scales are dissolved in NMP, ultrasonically vibrated intermittently at 200W intensity for 6 hours, and then ultrasonicated for 8 hours at 0°C. After sonication is completed, the unstripped black scale crystals are removed, the supernatant is centrifuged, and the precipitate is collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after every 5s interval, ultrasonic is applied for 1s; the method of removing the unstripped black scale crystals is: centrifugation at 6500rpm for 20min; supernatant centrifugation at 10000rpm, upper Centrifuge the clear liquid for 20 minutes.

[0042] Step two, prepare T7-PEG-NH 2 :Mal-PEG-NH 2 The solution is mixed with T7-Cys solution and then stir...

Example Embodiment

[0047] Example 2

[0048] This example is one of the examples for preparing BP-PEG-T7 / 5-ALA NSs.

[0049] Step 1. Preparation of BP NSs: Black scales are prepared by liquid phase stripping method to prepare BP NSs; among them, the liquid phase stripping method is: black scales are dissolved in NMP, ultrasonically vibrated intermittently at 300W intensity for 5 hours, and then ultrasonicated for 12 hours at 0°C. After sonication is completed, the unstripped black scale crystals are removed, the supernatant is centrifuged, and the precipitate is collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after every 2s interval, ultrasonic is applied for 2s; the method to remove the unstripped black scale crystals is: centrifugation at 6000rpm for 15min; supernatant centrifugation at 12000rpm, upper Centrifuge the clear liquid for 15 minutes.

[0050] Step two, prepare T7-PEG-NH 2 :Mal-PEG-NH 2 The solution is mixed with T7-Cys solution and then stirr...

Example Embodiment

[0055] Example 3

[0056] This example is one of the examples for preparing BP-PEG-T7 / 5-ALA NSs.

[0057] Step 1. Preparation of BP NSs: Black scales are prepared by liquid phase stripping method to prepare BP NSs; among them, the liquid phase stripping method is: black scales are dissolved in NMP, ultrasonically vibrated intermittently at an intensity of 260W for 8 hours, and then ultrasonicated for 6 hours at 0°C. After sonication is completed, the unstripped black scale crystals are removed, the supernatant is centrifuged, and the precipitate is collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after each interval of 4s, ultrasonic is applied for 4s; the method of removing the black scale crystals that is not peeled off is: centrifugation at 8000rpm for 30min; supernatant centrifugation at 8000rpm, upper Centrifuge the clear liquid for 30 minutes.

[0058] Step two, prepare T7-PEG-NH 2 :Mal-PEG-NH 2 The solution is mixed with T7-Cys sol...

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Abstract

The invention belongs to the fields of anti-tumor targeted drug research and development, and especially relates to a composition, a preparation method and an application thereof in a killing cell strain. The present invention provides a composition comprising black phosphorus, 5-aminolevulinic acid, polyethylene glycol, and a transferrin receptor binding peptide. The present invention also provides a preparation method of the above composition, and the present invention provides the application of the above composition or a product obtained by the above preparation method for killing SAS, HSC-2 and HSC-3 cell strains. According to the experimental determination, compared with a positive control drug, the product prepared by the technical solution provided by the invention has a significant inhibitory effect on the growth and reproduction of the tumor cell strain, and can further promote the apoptosis of the tumor cell. The invention provides the composition, the preparation method andthe application thereof in the killing cell strain, which solve the problem that the tumor targeting drug has difficulty in balancing the in vivo stability, the targeted aggregation ability and the carrier degradation performance, and other technical defects in the prior art.

Description

technical field [0001] The invention belongs to the field of research and development of anti-tumor targeted drugs, and in particular relates to a composition, a preparation method and its application in killing cell lines. Background technique [0002] Oral squamous cell carcinoma (OSCC) is one of the malignant tumors of the head and neck, and its incidence is increasing year by year. Currently, the main treatment for this disease is surgery. However, surgical treatment usually removes a large area of ​​oral tissues and organs, which seriously affects the quality of life of patients after surgery. Therefore, how to efficiently and non-invasively treat OSCC is one of the clinical problems that need to be solved urgently. [0003] Tumor sonodynamic therapy (SDT) is the use of ultrasound to activate sonosensitive drugs that selectively accumulate in tumor tissues. The sonosensitive drugs used for SDT treatment have problems such as unsatisfactory stability in vivo and limited...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/04A61K47/60A61K47/62A61P35/00
CPCA61K41/0033A61K47/02A61K47/60A61K47/62A61P35/00
Inventor 李楠黄盛兴南川川袁理孙海鹏陈伟璇赵跃宏
Owner SHENZHEN PEOPLES HOSPITAL
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