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Composition, preparation method and application thereof in killing cell strain

A composition-receptor binding technology, applied in the field of composition, application of killing cell lines, and preparation, can solve the problems of difficulty in taking into account in vivo stability, target aggregation ability and poor carrier degradation performance, etc., to improve tumor Targeted delivery effect, promotion of sonodynamic effect, effect of high biosafety

Active Publication Date: 2019-09-06
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention provides a composition, a preparation method and its application in killing cell lines, which are used to solve the problem of in vivo stability and targeting aggregation ability of tumor-targeted drugs in the prior art. And technical defects of poor carrier degradation performance

Method used

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  • Composition, preparation method and application thereof in killing cell strain
  • Composition, preparation method and application thereof in killing cell strain
  • Composition, preparation method and application thereof in killing cell strain

Examples

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Embodiment 1

[0040] This example is one example of preparing BP-PEG-T7 / 5-ALA NSs.

[0041] Step 1. Preparation of BP NSs: Black scales were prepared by liquid-phase exfoliation method to prepare BP NSs; wherein, the liquid-phase exfoliation method was: black scales were dissolved in NMP, and after intermittent ultrasonic vibration at 200W for 6 hours, continued ultrasonication at 0°C for 8 hours. After the ultrasonication was completed, the unstripped black scale crystals were removed, the supernatant was centrifuged, and the precipitate was collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after every interval of 5s, the ultrasonic action is 1s; The supernatant was centrifuged for 20 min.

[0042] Step 2. Preparation of T7-PEG-NH 2 : Mal-PEG-NH 2 The solution was mixed with the T7-Cys solution, stirred, and dried to obtain T7-PEG-NH 2 ;Mal-PEG-NH 2 The solvent of the solution is DMF, Mal-PEG-NH 2 The concentration of the solution is 15mg / ml...

Embodiment 2

[0048] This example is one example of preparing BP-PEG-T7 / 5-ALA NSs.

[0049] Step 1. Preparation of BP NSs: Black scales were prepared by liquid-phase exfoliation method to prepare BP NSs; wherein, the liquid-phase exfoliation method was: black scales were dissolved in NMP, ultrasonically vibrated intermittently at 300W for 5 hours, and then ultrasonicated at 0°C for 12 hours. After the ultrasonication was completed, the unstripped black scale crystals were removed, the supernatant was centrifuged, and the precipitate was collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after every interval of 2s, ultrasonic action is applied for 2s; the method of removing the unstripped black scale crystals is: centrifuged at a speed of 6000rpm for 15min; the speed of centrifugation of the supernatant is 12000rpm, The supernatant was centrifuged for 15 minutes.

[0050] Step 2. Preparation of T7-PEG-NH 2 : Mal-PEG-NH 2 The solution was mixed wit...

Embodiment 3

[0056] This example is one example of preparing BP-PEG-T7 / 5-ALA NSs.

[0057] Step 1. Preparation of BP NSs: Black scales were prepared by liquid-phase exfoliation method to prepare BP NSs; wherein, the liquid-phase exfoliation method was: black scales were dissolved in NMP, ultrasonically vibrated intermittently at 260W for 8 hours, and then ultrasonicated for 6 hours at 0°C. After the ultrasonication was completed, the unstripped black scale crystals were removed, the supernatant was centrifuged, and the precipitate was collected as BP NSs. In the present embodiment, the method of ultrasonic intermittent vibration is: after each interval of 4s, ultrasonic action is applied for 4s; the method of removing unstripped black scale crystals is: centrifuged at a speed of 8000rpm for 30min; the speed of centrifugation of the supernatant is 8000rpm, and The supernatant was centrifuged for 30 min.

[0058] Step 2. Preparation of T7-PEG-NH 2 : Mal-PEG-NH 2 The solution was mixed wit...

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Abstract

The invention belongs to the fields of anti-tumor targeted drug research and development, and especially relates to a composition, a preparation method and an application thereof in a killing cell strain. The present invention provides a composition comprising black phosphorus, 5-aminolevulinic acid, polyethylene glycol, and a transferrin receptor binding peptide. The present invention also provides a preparation method of the above composition, and the present invention provides the application of the above composition or a product obtained by the above preparation method for killing SAS, HSC-2 and HSC-3 cell strains. According to the experimental determination, compared with a positive control drug, the product prepared by the technical solution provided by the invention has a significant inhibitory effect on the growth and reproduction of the tumor cell strain, and can further promote the apoptosis of the tumor cell. The invention provides the composition, the preparation method andthe application thereof in the killing cell strain, which solve the problem that the tumor targeting drug has difficulty in balancing the in vivo stability, the targeted aggregation ability and the carrier degradation performance, and other technical defects in the prior art.

Description

technical field [0001] The invention belongs to the field of research and development of anti-tumor targeted drugs, and in particular relates to a composition, a preparation method and its application in killing cell lines. Background technique [0002] Oral squamous cell carcinoma (OSCC) is one of the malignant tumors of the head and neck, and its incidence is increasing year by year. Currently, the main treatment for this disease is surgery. However, surgical treatment usually removes a large area of ​​oral tissues and organs, which seriously affects the quality of life of patients after surgery. Therefore, how to efficiently and non-invasively treat OSCC is one of the clinical problems that need to be solved urgently. [0003] Tumor sonodynamic therapy (SDT) is the use of ultrasound to activate sonosensitive drugs that selectively accumulate in tumor tissues. The sonosensitive drugs used for SDT treatment have problems such as unsatisfactory stability in vivo and limited...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/04A61K47/60A61K47/62A61P35/00
CPCA61K41/0033A61K47/02A61K47/60A61K47/62A61P35/00
Inventor 李楠黄盛兴南川川袁理孙海鹏陈伟璇赵跃宏
Owner SHENZHEN PEOPLES HOSPITAL
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