Preparation method of plant cell enzymolysis extract for treating female HPV
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A technology of HPV virus and plant cells, which is applied in the field of preparation of plant cell enzymatic extracts, can solve the problems of human side effects, elimination, difficult immune system, etc., and achieve the effects of high extraction rate, low production cost, and easy method and operation
Pending Publication Date: 2019-09-10
任辛
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Problems solved by technology
However, most viruses will be cleared by the immune system in women, and some are more stubborn and difficult to be cleared by the immune system, which may lead to cervical lesions
After infection with HPV, the current conventional treatment method is to use a large amount of antibiotics, but all of them will have side effects on the human body, and make the human body develop drug resistance, which is not conducive to human health
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Embodiment 1
[0021] The preparation method of the plant cell enzymolysis extract for the treatment of female HPV virus of the present embodiment comprises the following steps:
[0022] S1: Peel the fresh garlic, wash the lemon, remove the seeds and slice, mix the garlic and the lemon, crush them, and pass through a 20-mesh sieve to obtain the mixture for later use;
[0023] S2: Add edible mature vinegar to the prepared mixture according to the mass ratio of 1:1, place it in an enzymatic hydrolysis container, add amylase, stir at high speed for 30 minutes, stir once a day, and continue stirring for 5 days;
[0024] Wherein, the solution concentration of edible mature vinegar used for enzymolysis of garlic and lemon is 30%, and the enzymolysis temperature is 20°C. The feeding ratio of amylase for enzymolysis of garlic and lemon is 10% of the total amount in the enzymolysis container.
[0025] S3: adding cell fusion enzyme to S2, resting for 30 days;
[0026] Wherein, the cell fusion enzyme...
Embodiment 2
[0029] The preparation method of the plant cell enzymolysis extract of the treatment female HPV virus of the present embodiment, its preparation method comprises the following steps:
[0030] S1: Peel the fresh garlic, wash the lemon, remove the seeds and slice, mix the garlic and the lemon, crush them, and pass through a 20-mesh sieve to obtain the mixture for later use;
[0031] S2: Add edible mature vinegar to the prepared mixture according to the mass ratio of 1:1, place it in an enzymatic hydrolysis container, add amylase, stir at high speed for 20 minutes, stir once a day, and continue stirring for 6 days;
[0032] Wherein, the solution concentration of edible mature vinegar used for enzymolysis of garlic and lemon is 30%, and the enzymolysis temperature is 25°C. The feeding ratio of amylase for enzymolysis of garlic and lemon is 10% of the total amount in the enzymolysis container.
[0033] S3: adding cell fusion enzyme to S2, resting for 35 days;
[0034] Wherein, th...
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Abstract
The invention provides a preparation method of a plant cell enzymolysis extract for treating a female HPV, and relates to the technical field of biological pharmacy. The preparation method of the plant cell enzymolysis extract comprises the steps that fresh garlic is peeled, lemons are thoroughly cleaned and sliced after seed removal, and the garlic and the lemon are mixed and crushed and pass through a 10-20 mesh sieve to obtain a mixture for use; according to the mass ratio of 1:1, edible aged vinegar is added in the prepared mixture, an obtained solution is placed in an enzymolysis container, starch protease is added, and high-speed rotary stirring is conducted for 20-30 minutes, wherein stirring is conducted once every day and continuously conducted for 5-6 days; then a cell fusion enzyme is added in the container, and standing is conducted for 30-40 days; separation and purification are conducted at room temperature, an extract is subjected to clarification filtration to be 200 mesh, sodium carboxymethyl cellulose is added according to the mass ratio of 1:0.25 for mixing, and the plant cell enzymolysis extract can be obtained. The obtained extract can strongly kill the human papilloma virus (HPV), block and reduce the incidence of female cervical cancer and effectively treat female reproductive system diseases.
Description
technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for preparing a plant cell enzymolysis extract for treating female HPV virus. Background technique [0002] Human papillomavirus is a kind of papillomavacuolating virus A belonging to Papovaviridae family. It is a spherical DNA virus that can cause squamous epithelial proliferation of human skin and mucous membranes. Symptoms such as common warts and genital warts (condyloma acuminatum). With the rapid increase in the incidence of genital warts in venereal diseases and the increase of cervical cancer and anal cancer, human papillomavirus (hpv) infection has attracted more and more attention. On October 27, 2017, the list of carcinogens released by the International Agency for Research on Cancer of the World Health Organization was initially compiled for reference. In the list of carcinoids. At present, more than 130 kinds have been isolated, and different t...
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