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Bacterial cellulose hydrogel dressing for diabetic wound surface and preparation method thereof

A technology of bacterial cellulose and hydrogel, applied in medical science, bandages, etc., can solve the problem that dressings are not suitable for diabetic wounds, and achieve the effects of reducing blood sugar on wounds, ensuring safety, and promoting healing rates

Active Publication Date: 2019-09-10
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this patent, because: first, bacterial cellulose is loaded with collagen; second, the dressing involved is a dry dressing that has been freeze-dried; third, this patent does not involve any small molecule drug loading, so , the dressing disclosed in this patent is not suitable for diabetic wounds

Method used

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  • Bacterial cellulose hydrogel dressing for diabetic wound surface and preparation method thereof
  • Bacterial cellulose hydrogel dressing for diabetic wound surface and preparation method thereof
  • Bacterial cellulose hydrogel dressing for diabetic wound surface and preparation method thereof

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preparation example Construction

[0051] The preparation method of the bacterial cellulose hydrogel dressing for diabetic wounds of the present invention comprises the following four steps;

[0052] The first step is to modify the raw material bacterial cellulose BC to obtain aldehyde-ylated bacterial cellulose BC-CHO;

[0053] The second step is to further oxidatively modify the aldehyde-ylated bacterial cellulose BC-CHO to obtain carboxylated bacterial cellulose BC-COOH;

[0054] The third step is to react the carboxylated BC-COOH with aminated glucose oxidase to prepare a bacterial cellulose-glucose oxidase BC-GOD hydrogel, wherein the glucose oxidase and the bacterial cellulose skeleton are separated by a amide bonds are connected;

[0055] The fourth step is to soak the BC-GOD gel in the solution of the hematopoietic stem cell mobilization drug AMD3100 overnight to obtain the BC-GOD hydrogel loaded with AMD3100.

Embodiment 1

[0057] 5g of raw bacterial cellulose gel (solid content of 0.27g) was cut into small pieces and placed in 250mL of a solution containing 0.71g of sodium periodate and stirred for 24h, wherein the molar ratio of sodium periodate to bacterial cellulose structural unit was 2 : 1. After the reaction was completed, the reaction was terminated with 10 mL of ethylene glycol and the gel was washed to prepare the aldehydeylated bacterial cellulose BC-CHO. The BC-CHO was then placed in 250 mL of a mixed solution containing sodium chlorite and hydrogen peroxide and stirred for 12 h, wherein the molar ratio of the total aldehyde groups of sodium chlorite to BC-CHO was 2:1, and the ratio of sodium chlorite to peroxide was 2:1. The molar ratio of hydrogen was 1:1, and the gel was washed after the end to prepare carboxylated bacterial cellulose BC-COOH.

[0058] A total of 100 mL of 2 mg / mL glucose oxidase solution was prepared and reacted with 0.01 mol of NAS (acryloyloxysuccinimide) at pH ...

Embodiment 2

[0062] 5g of raw bacterial cellulose gel (solid content of 0.27g) was cut into small pieces and placed in 250mL of sodium periodate solution containing 0.71g and stirred for 18h, wherein the molar ratio of sodium periodate to bacterial cellulose structural unit was 2 : 1. After the reaction was completed, the reaction was terminated with 20 mL of ethylene glycol and the gel was washed to prepare the aldehydeylated bacterial cellulose BC-CHO. The BC-CHO was then placed in 250 mL of a mixed solution containing sodium chlorite and hydrogen peroxide and stirred for 24 hours, wherein the molar ratio of the total aldehyde groups of sodium chlorite to BC-CHO was 2:1, and the ratio of sodium chlorite to peroxide was 2:1. The molar ratio of hydrogen was 1:1, and the gel was washed after the end to prepare carboxylated bacterial cellulose BC-COOH.

[0063] The carboxylated bacterial cellulose BC-COOH was placed in 250 mL of pH 5.0 phosphate buffer, and the catalysts 1-ethyl-(3-dimethyla...

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Abstract

The invention provides a bacterial cellulose hydrogel dressing for a diabetic wound surface and a preparation method thereof. The hydrogel dressing comprises carboxylation bacterial cellulose and amination glucose oxidase, and the carboxylation bacterial cellulose and the amination glucose oxidase are in amido bond connection. The hydrogel dressing further comprises plerixafor; the plerixafor is loaded inside the hydrogel dressing. Glucose oxidase is grafted to the surface of the hydrogel dressing through amido bonds, the gluconic effect in the wound surface can be lowered, and glucose oxidasecannot enter blood circulation; meanwhile, the stem cell mobilizing drug AMD3100 is loaded in the modified cellulose dressing through the physical adsorption effect, the medicine can be released intobody fluid when the dressing makes contact with the wound surface, and the drug is used for prompting generation of new vessels at the wound surface.

Description

technical field [0001] The present invention relates to the technical field of biomedical materials, in particular, to a bacterial cellulose hydrogel dressing for diabetic wounds and a preparation method thereof. The present invention provides a novel bacterial cellulose wound dressing and its application in diabetic wound healing Applications. Background technique [0002] Diabetic wound ulcer is a common complication of diabetes, which is common in the feet of diabetic patients. Due to high blood sugar levels, wounds in diabetics heal at a much slower rate than healthy people. The healing process of wounds is divided into four stages: coagulation period, inflammation period, proliferation period and remodeling period. However, blood vessels and nerves in diabetic wounds have lesions, resulting in slow or impossible regeneration of blood vessels and nerves in the wound proliferation period, which is the cause of The main reason for the slow healing rate of diabetic wounds...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0061A61L26/0066A61L26/008A61L2300/204A61L2300/254A61L2300/412A61L2300/45A61L2300/80C08L1/02
Inventor 朱新远许冠哲金鑫童刚生
Owner SHANGHAI JIAO TONG UNIV
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