A strain of Trichoderma longibrachiata md30 and its bio-organic fertilizer
A technology of bio-organic fertilizer and Trichoderma longibranchus, which is applied in the field of agricultural microorganisms to achieve stable growth-promoting ability, increase growth speed, and obvious growth-promoting effect
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Embodiment 1
[0023] Embodiment 1, isolation and identification of functional strains
[0024] Put 5g of soil sample in a Erlenmeyer flask filled with 45ml of sterile water, shake at 28°C for 30min (170r / min) to make a soil suspension. Take 0.1mL of soil suspension and place it on a Trichoderma selective plate and spread it, place it in an incubator at 28°C for 48 hours, observe the growth of colonies on the plate, select different colonies for purification, and inoculate the purified strains on PDA medium and stored in a refrigerator at 4°C until use.
[0025] The selected Trichoderma strains were inoculated on PDA medium, placed in 28°C, 37°C and 40°C incubators respectively, observed after 5 days of cultivation, and the high-temperature Trichoderma that could grow normally at 40°C was screened out.
[0026] Through screening, a Trichoderma strain was finally obtained and named MD30. The strain MD30 grew faster on the PDA plate, with fluffy white hyphae, produced a large number of green ...
Embodiment 2
[0027] Growth measurement under embodiment 2, different temperatures
[0028] The strain MD30 (CGMCC No.17467) was inoculated on PDA medium at different temperatures, and its growth status was observed. The set temperature is 37°C, 40°C and 42°C, and the control is Trichoderma Guizhou NJAU4742 strain with the preservation number CGMCC NO.12166, which is disclosed in CN108675895A.
[0029] It was found that under the high temperature condition of 38°C, the strain MD30 could still grow, but the 4742 strain could not grow normally; under the high temperature condition of 40°C, the strain MD30 could still grow, but the 4742 strain could not grow; and 4742 strains were unable to grow.
[0030] Table 1. Growth rate of Trichoderma mycelium at 40°C
[0031]
[0032] Embodiment 2, the generation of functional bacteria biological fertilizer
[0033] Inoculate the bacterial strain MD30 (CGMCC No.17467) into the PDA culture medium for liquid fermentation. The conditions for fermenta...
Embodiment 3
[0035] Embodiment 3, potted plant growth-promoting effect test
[0036] 3.1 Application effect of growth-promoting biofertilizer on cucumbers (first and second seasons)
[0037] The growth-promoting pot experiment was carried out in the greenhouse of the National Organic Fertilizer Engineering Technology Research Center (Yixing). The test soil was healthy sandy soil without pathogenic bacteria, and the tested cucumber variety was Lufeng cucumber without pathogenic bacteria. Soak cucumber seeds in normal temperature water for 1 hour, place them on moist gauze, and germinate them in a 30°C incubator for 12-24 hours. After more than 75% of the seeds have germinated, select the dewy seeds and move them to the seedling tray for seedling cultivation. After leaves, select seedlings with consistent growth and transplant them into pots. Two treatments were set up in the experiment: 1) CK, no fertilizer was applied; 2) OF treatment, ordinary organic fertilizer was applied; 3) MD30 trea...
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