Bacillus haynesii LY-23, bacterial agent, application of bacterial agent and product applying bacillus haynesii LY-23

A technology of Bacillus spp. and LY-23, applied in the field of microorganisms, can solve the problems that the industrial production potential has not been fully utilized, donkeys are prone to digestive system diseases, and the source of core bacteria is unclear, etc., so as to reduce digestive system diseases. Probability, improve immunity, good effect of enzyme activity

Active Publication Date: 2019-09-10
SHENYANG BOYANG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the traditional breeding of donkeys is mainly based on crop straw, green hay, bran and cakes, supplemented by a small amount of concentrate, and there are many cases where the feed ingredients are simple and the nutritional ratio is unreasonable.
During the growing period, a large amount of concentrate is supplemented, and the roughage is mostly low-quality roughage such as bean stalks and corn stalks. The qu

Method used

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  • Bacillus haynesii LY-23, bacterial agent, application of bacterial agent and product applying bacillus haynesii LY-23
  • Bacillus haynesii LY-23, bacterial agent, application of bacterial agent and product applying bacillus haynesii LY-23
  • Bacillus haynesii LY-23, bacterial agent, application of bacterial agent and product applying bacillus haynesii LY-23

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Isolation, purification and biochemical characteristics of Bacillus haynesii from donkey:

[0057] (I) Medium for separation: beef extract peptone medium, the medium components are as follows: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 1000mL distilled water, pH 7.2, sterilized at 121°C for 30min.

[0058] (II) Separation method:

[0059] (1) Sample collection and microbial isolation: Select a healthy adult black donkey from the black donkey breeding base in Dong'e County, collect the contents of the large intestine, quickly put it into a sterilized screw-top test tube with a liquid medium, and bring it back to the experiment Immediately after the chamber, the gradient dilution was carried out, and three plates were used for each gradient, separated and cultured by pouring method, and cultured upside down at 37°C.

[0060] (2) Separation and purification: take the above plate with various bacteria, select a single colony of Bacillus, and use continuous str...

Embodiment 2

[0070] Carbohydrate fermentation experiment of Bacillus hainei LY-23: Carry out sugar fermentation experiment using common bacterial biochemical identification tube (Guangdong Huankai Biotechnology Co., Ltd.). The results show that the bacteria can utilize various carbohydrates widely present in nature such as glucose, fructose, maltose, sucrose, cellobiose, etc. The results are shown in Table 1. It can also be used as an alternative strain for fermenting plant-based feed.

[0071] Table 1 Carbohydrate fermentation by Bacillus hainei LY-23

[0072]

[0073]

Embodiment 3

[0075] Enzyme production experiment of Bacillus hainei LY-23.

[0076] Basal medium: beef extract peptone medium; milk powder medium (protease): A: 3.2g skimmed milk powder, add distilled water to 120mL, sterilize at 121°C for 1min; B: 3.2g agar, add distilled water to 120mL, 121°C Sterilize for 20 minutes; sterilize A and B respectively, cool to 60°C and mix well, then pour onto a plate for detection of protease activity.

[0077] On the solidified and cooled medium plate, use the Oxford cup method to inoculate the target strain, and measure the size of the hydrolysis circle on the milk powder medium plate to determine whether it can secrete protease.

[0078] Amylase detection: add the bacteria solution to be tested into the sample wells of the qualitative medium solid plate, set up 3 repetitions, incubate at 37°C for 20-24h, add 0.1mL iodine solution to the amylase qualitative medium, and evenly mix the iodine solution Cover the plate with coating, let it stand for 10 minu...

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Abstract

The invention provides bacillus haynesii LY-23, a bacterial agent, application of the bacterial agent and a product applying the bacillus haynesii LY-23, and relates to the field of microorganisms. The bacillus haynesii LY-23 is separated from the large intestine content of a healthy adult black donkey, multiple carbohydrates can be utilized, the bacillus haynesii LY-23 has good enzymatic activity, wherein proteinase activity, amylase activity and cellulase activity are good, and especially, the bacillus haynesii LY-23 has extremely high cellulase activity; the bacillus haynesii LY-23 furtherincreases the rate of digesting nutrient substances in feed by animals and increases the content of serum immune globulin IgG in the animals. Based on the functions of the bacillus haynesii LY-23, the bacillus haynesii LY-23 can be applied to fermentation, preparation of feed additives, feed, products capable of improving the intestinal tract environment of equus animals and products capable of improving the immunity of the equus animals.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a bacillus hainei bacillus LY-23, a bacterial agent and its application and products using the same. Background technique [0002] Donkeys belong to the order Perissodactyla, Equidae, Equus, herbivorous animals. Our country has a long history of donkey domestication, and many excellent local breeds have been formed. [0003] With the development of the industry, the donkey breeding industry has become an important part of my country's special animal breeding industry, forming a complete industrial chain and playing an important role in increasing farmers' income and driving poverty alleviation. However, the research on domesticated donkeys started late in my country, and the basic research is less, mostly focusing on the prevention and control of common diseases, feeding management technology and fattening performance research. There are few domestic reports on the research on the compo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/20C12R1/07
CPCA23K10/18A23K50/20C12N1/20C12N1/205C12R2001/07
Inventor 李光玉刘晗璐王卓司华哲张婷
Owner SHENYANG BOYANG FEED
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