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Dry powder bacterial agent for removing low-concentration copper ions in wastewater

A low-concentration, dry powder technology, applied in the field of environmental microorganisms, can solve problems such as difficulties in practical and industrial applications, inability to tolerate heavy metals, and few microbial resources, and achieve industrial applications, large bacterial loads, and high bacterial activity.

Pending Publication Date: 2019-09-10
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the lack of microbial resources, low removal efficiency, and inability to tolerate the toxicity of heavy metals are the bottlenecks that limit the application of microbial live cell adsorption. As a result, biosorption is only in the laboratory stage, and there are still difficulties in practical and industrial applications.

Method used

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  • Dry powder bacterial agent for removing low-concentration copper ions in wastewater
  • Dry powder bacterial agent for removing low-concentration copper ions in wastewater
  • Dry powder bacterial agent for removing low-concentration copper ions in wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The dry powder bacterial agent for treating wastewater containing copper ions is prepared according to the following process:

[0032] After the self-isolated Bacillus cereus was isolated and purified by the plate streaking method, the strain was rejuvenated, and the Bacillus cereus was cultured in LB medium with a transfer ratio of 1% to the logarithmic growth phase OD 600 =1 to obtain a copper-tolerant bacterial fermentation broth.

[0033] The immobilization carrier is selected as 150 mesh talcum powder.

[0034] The weight ratio of the copper-resistant bacterial fermentation broth to the immobilized carrier was 60:1. The copper-resistant bacterial fermentation broth was centrifuged at 8000rpm for 5min, and the supernatant was removed and washed repeatedly with phosphate buffered saline (PBS) and centrifuged 3 times. Wash and remove the bottom of the supernatant sludge and resuspend it into the LB medium after high temperature sterilization, and mix the resuspended ...

Embodiment 2

[0036] The dry powder bacterial agent for treating wastewater containing copper ions is prepared according to the following process:

[0037] After the self-isolated Bacillus cereus was isolated and purified by the streaking method on the plate, the strain was rejuvenated. The Bacillus cereus was transferred to LB medium at a transfer ratio of 5%, and cultured until the logarithmic growth phase OD600=1.1 to obtain copper-resistant bacteria fermentation broth.

[0038] The immobilized carrier is 90 mesh talcum powder.

[0039] The weight ratio of the copper-resistant bacterial fermentation broth to the immobilized carrier was 60:1. The copper-resistant bacterial fermentation broth was centrifuged at 8000rpm for 5min, and the supernatant was removed and washed repeatedly with phosphate buffered saline (PBS) and centrifuged 3 times. Wash and remove the supernatant from the bottom of the sludge and resuspend the LB medium after high temperature sterilization, and mix the resuspen...

Embodiment 3

[0041]The dry powder bacterial agent for treating wastewater containing copper ions is prepared according to the following process:

[0042] After the self-isolated Bacillus cereus was isolated and purified by the streaking method on the plate, the strain was rejuvenated. The Bacillus cereus was transferred to LB medium at a transfer ratio of 5%, and cultured until the logarithmic growth phase OD600=1.1 to obtain copper-resistant bacteria fermentation broth.

[0043] 80 mesh talcum powder was selected as the immobilization carrier.

[0044] The weight ratio of the copper-resistant bacterial fermentation broth to the immobilized carrier was 60:1. The copper-resistant bacterial fermentation broth was centrifuged at 8000rpm for 5min, and the supernatant was removed and washed repeatedly with phosphate buffered saline (PBS) and centrifuged 3 times. Wash and remove the supernatant from the bottom of the sludge and resuspend the LB medium after high temperature sterilization, and m...

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Abstract

The invention relates to a dry powder bacterial agent for removing low-concentration copper ions in wastewater. The dry powder bacterial agent is prepared by mixing a fermented copper-resistance bacterium and an immobilization carrier, wherein the copper-resistance bacterium is independently-separated bacillus sp., the bacillus sp. is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.7368. The invention provides new application of the CR1 bacillus sp. with the preservation number of CGMCC NO.7368. The efficient dry powder bacterial agent prepared by mixing a fermentation liquid of the bacillus sp. and the immobilization carrier is suitable for treating copper-polluted wastewater with the low concentration of 2-16 mg / L, the treatment time is short, the effluent concentration is low, and industrialized application is achieved.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a dry powder bacterial agent for removing low-concentration copper ions in wastewater. Background technique [0002] Environmental pollution is one of the most serious challenges facing mankind in the 21st century. Among them, heavy metal pollution has the characteristics of long-term, cumulative, latent and irreversible, and its impact on human health and ecological security is more serious and far-reaching, and has become the focus of global attention. Copper mine mining, smelting, electroplating industry and electronics industry discharge a large amount of copper-containing wastewater every year, and the copper ion content in wastewater is about 15mg / L. The discharge of heavy metal copper ions is very harmful to water and soil. Copper is one of the essential trace elements for the human body, but if copper exceeds the standard in the human body, it will ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34C12R1/085C02F101/20
CPCC12N11/14C02F3/34C02F2101/20
Inventor 王小雨韩业轩
Owner NORTHEAST NORMAL UNIVERSITY
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