Experimental device for automatic simulation of gastrointestinal continuous digestion and application method

Abstract

The invention provides a device capable of realizing automatic simulation of gastrointestinal continuous digestion and an application method of the device. The device comprises a stomach reactor, a stirrer, a constant-temperature water circulation system, a gastrointestinal fluid conveying system, a gastric emptying control device, an automatic pH titration device, a small intestine reactor and asample receiving device. According to the application method, to-be-digested food is conveyed into a stomach digester, stomach digestive fluid is input at a constant speed, the stirrer simulates stomach peristalsis, a micropore glass plate at the bottom of the stomach reactor simulates a pylorus to realize stomach "sieving emptying", the to-be-digested food is conveyed into a pH regulation chamberfor automatic pH regulation, small intestine digestion is simulated in the small intestine reactor provided with an internally-arranged latex sleeve, and the pressure-changing water circulation system is adopted to realize regular vibration of the latex sleeve so as to simulate the stomach peristalsis. Through the device and the application method, the whole reaction process can be continuous andautomatic under the situation that parameters are set; and moreover, the device is simple in structure, free of a complicated control system and easy to assemble and can be used to realize a digestion simulation experiment in a scientific research experiment.

Description

technical field [0001] The invention relates to an experimental device for automatically simulating gastrointestinal continuous digestion and a method for using it, belonging to the technical field of biological experiment instruments. Background technique [0002] At present, more than ten relatively mature in vitro digestion simulation methods have been established in the world, such as PBET method, in vitro gastrointestinal method (IVG), simplified bioaccessible extraction method (SBET), human intestinal microbial ecological simulation system (SHIME), Model methods widely recognized by researchers, such as TNO intestinal model (TIM) and dynamic gastrointestinal tract simulator (SIMGI), have been developed into commercial simulated digestive devices, but they are relatively expensive and poor in popularity. However, the research on devices and methods for simulating digestion in vitro started relatively late in China, including: "A device for simulating digestive tract sys...

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Problems solved by technology

However, most of the current in vitro simulating gastrointestinal integrated digestion models have complex systems, many control components, and high production costs, which are not conducive to general laboratory research and use, and their popularity is poor.

Moreover, most of these devices do not describe the parameters of the operation process in detail enough, and do not take into account the "sieving emptying" effect of the pyloric position in the gastric digestion process and the "peristaltic propulsion" digestion process of the small intestine, and do not consider food in the digestive process. continuous flow process in

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