Microbial organic fertilizer and applications thereof

An organic fertilizer and microbial technology, applied in the direction of microorganisms, microorganism-based methods, applications, etc., can solve the problems of soil microbial flora destruction, limited control effect, aggravation of soil-borne diseases, etc., to promote germination and root growth, improve Bioactivity, effect of increasing tuber yield

Inactive Publication Date: 2019-09-17
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control is a method commonly used at present, and the control effect is good, but it will cause problems such as damage to the soil microbial flora, lead to the degradation of the disease-suppressing soil and lead to the degradation of the disease-producing soil, and aggravate the occurrence of soil-borne diseases. The agricultural control The control effect is limited

Method used

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  • Microbial organic fertilizer and applications thereof
  • Microbial organic fertilizer and applications thereof
  • Microbial organic fertilizer and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, isolation and identification of functional strains

[0033] The rhizosphere soil and root system of healthy plants were collected from the plots affected by Fusarium wilt in cucumber and vegetable greenhouses in Baiyin District, Baiyin City, Gansu Province. Weigh a total of 5g of rhizosphere soil and root system, among which the root system is cut into 1mm segments with sterile scissors, and placed in 90ml of sterile water filled with glass beads in a sterile environment, at 28°C, 180rpm·min -1 Incubate on a shaking table for 0.5h, carry out gradient dilution, take 10 -4 、10 -5 、10 -6 0.1mL of the diluted solution was applied and cultured at a constant temperature of 28°C for 3 days. A total of 195 strains of bacteria with different shapes and colors were isolated. The plate confrontation test was carried out on the selected bacteria (references for the plate confrontation test method: Qiao Shengwei. A study on the isolation and identification of an ant...

Embodiment 2

[0040] Embodiment 2, the production of functional bacteria bacterial liquid

[0041]Activate the strain QHZ-3 and inoculate it into LB culture medium for liquid fermentation production. The conditions are: pH 7.0-7.2, fermentation temperature 28±2°C, rotation speed 170r / min, fermentation to obtain QHZ-3 bacterial liquid, bacterial liquid The number of effective viable bacteria in the medium is ≥1.0×10 9 CFU / mL.

[0042] The preparation method of the LB culture medium used is as follows (take the preparation of 1L medium as an example): 950ml of distilled water, 10g of peptone, 5g of yeast extract powder, and 10g of NaCl are mixed evenly, then the volume is adjusted to 1000mL with distilled water, and the pH is adjusted with 5mol / L NaOH To 7.0-7.2, sterilize at 121°C for 30min.

Embodiment 3

[0043] Embodiment 3, the development of microbial organic fertilizer

[0044] Add animal source amino acid stock solution (Jiangyin Lianye Biotechnology Co., Ltd.) to the organic fertilizer (Baiyin Xinhao Biotechnology Co., Ltd., cow dung organic fertilizer) by 15% (V / m, ml / g) of the organic fertilizer quality, Ferment 5d, when its pH value is 6.5-6.8, by 5% (V / m, ml / g) the QHZ-3 bacterium liquid of above-mentioned preparation is inoculated in amino acid organic fertilizer, carries out secondary solid fermentation, and fermentation temperature is 40±2°C, turn the compost once a day during the fermentation process, and ferment for 7 days to make the number of effective viable bacteria of QHZ-3 ≥0.2×10 9 cfu / g, the water content was evaporated to 25% at a temperature of 50°C to obtain microbial organic fertilizers for pot experiments.

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Abstract

The invention discloses a microbial organic fertilizer, which is prepared from the following steps: inoculating Bacillus atrophaeus QHZ-3 having a preservation number of CGMCC No.13639 in a LB culture liquid, and carrying out liquid fermentation to obtain a QHZ-3 bacterial liquid having the effective viable count of more than or equal to 1*10<9> CFU/mL; preparing an amino acid organic fertilizer, wherein an animal source amino acid stock solution is added to an organic fertilizer according to a ratio of 15 ml/100 g, and fermenting is performed for 5 d to achieve the pH value of 6.5-6.8; and inoculating the QHZ-3 bacterial liquid in the amino acid organic fertilizer according to a ratio of 5 ml/100 g, and carrying out secondary solid fermentation, such that the effective viable count of the QHZ-3 is more than or equal to 0.2*10<9> cfu/g, and the water content is evaporated to less than 30%. According to the present invention, the microbial organic fertilizer has antagonistic effect on Rhizoctonia solani, and can effectively promote the growth of potato plants and increase the tuber yield.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a microbial organic fertilizer and its application. Background technique [0002] Potatoes are the fourth largest food crop after rice, wheat and corn, and are also recognized as a complete food in the world. According to FAO data, my country is the largest producer of potatoes. However, in recent years, the continuous planting of potatoes and the application of a large amount of chemical fertilizers have caused the accumulation of pathogenic bacteria in the soil and caused frequent occurrence of soil-borne diseases, which has led to an increase in production costs and reduced the yield and quality of potatoes. [0003] Potato soil-borne diseases are increasing worldwide, especially potato black spot disease caused by Rhizoctonia solani infection, which has become the main disease affecting potato yield and commodity value. In my country's main potato producing ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C12N1/20C12R1/07C05G3/80
CPCC05C11/00C12N1/20C05G3/80C05F3/00C05F11/08
Inventor 邱惠珍罗超越张春红王友玲关小敏马兴邱晓丽
Owner GANSU AGRI UNIV
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