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A strain of high folic acid producing Lactobacillus plantarum gslp-7 and its application

A technology of GSLP-7 and Lactobacillus plantarum, applied in the field of microorganisms, can solve the problems of high cost, low absorption and utilization rate of folic acid in human body, and difficult promotion

Active Publication Date: 2021-04-06
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improving food structure has the problems of being difficult to promote due to geographical restrictions and high cost. For example, the consumption of vegetables and fruits in Northwest and Inner Mongolia is generally low, and the absorption and utilization of folic acid in some fruits and vegetables are low, resulting in high demand for folic acid such as pregnant women. A large amount of intake is required, causing pressure on the stomach and digestive system

Method used

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  • A strain of high folic acid producing Lactobacillus plantarum gslp-7 and its application
  • A strain of high folic acid producing Lactobacillus plantarum gslp-7 and its application
  • A strain of high folic acid producing Lactobacillus plantarum gslp-7 and its application

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Experimental program
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Effect test

Embodiment 1

[0035] Example 1, Isolation and Identification of Lactobacillus plantarum GSLP-7

[0036] 1. Separation and purification of bacteria

[0037] Isolation of lactic acid bacteria from Chinese traditional fermented dairy product Zangling mushroom. Take 5g of the sample to be separated and add it to 45mL of normal saline, oscillate and mix, and then dilute step by step. Take 50μL of the diluted bacterial solution and spread it on MRS (MRS medium formula: 10g of egg white, 10g of beef extract, 5g of yeast extract, glucose 20g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Tween 80 1mL, diamine citrate 2g, magnesium sulfate heptahydrate 0.58g, manganese sulfate tetrahydrate 0.25g, distilled water 1000ml, pH6.2-6.4.) Agar plate ( Add 1.5% mass fraction of agar) to MRS, and culture under anaerobic conditions at 37°C for 48-72h. Streak culture was repeated 3 times, and a single colony was picked. One of the strains screened by the above method is coded as GSLP.

[0038] 2. R...

Embodiment 2

[0052] Embodiment 2, identification of other properties of Lactobacillus plantarum GSLP-7

[0053] 1. Identification of acid resistance

[0054] The activated Lactobacillus plantarum GSLP-7 (CGMCC No.17171) strain was inoculated into the MRS medium with pH=2.0 and 3.0 according to the inoculum amount of 3% (v / v), and cultured at a constant temperature of 37°C. At 1h, 3h and 6h, the number of viable bacteria in the medium was measured by the spread plate counting method, and the MRS medium (pH ~ 6.8) without adjusting the pH was used as a blank, and the test was repeated twice.

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Abstract

The invention discloses a strain of high-folate-producing Lactobacillus plantarum GSLP‑7 and its application. The invention provides Lactobacillus plantarum (Lactobacillus plantarum) GSLP‑7, whose preservation number in the General Microorganism Center of China Microorganism Culture Collection Management Committee is CGMCC No. 17171. Lactobacillus plantarum (Lactobacillus plantarum) GSLP-7 provided by the present invention can efficiently synthesize folic acid. When cultured in SDM medium at 37° C. for 16 hours, the output of extracellular folic acid reaches 3.5 μg / mL, and the output of intracellular folic acid reaches 9.7 μg / mL. mL. The plant lactobacillus (Lactobacillus plantarum) GSLP-7 of the present invention is of great significance to the development and production of fermented foods rich in folic acid.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of high folic acid-producing Lactobacillus plantarum GSLP-7 and its application. Background technique [0002] Folic acid is an important vitamin in organisms. As a carrier of one-carbon units, it participates in the methylation reaction, which is closely related to the synthesis of DNA, RNA and protein in the body, and plays a very important role in the metabolic process of the body. role. Folic acid deficiency is common in pregnant women, infants, and middle-aged and elderly people in my country. The "National Nutrition Plan (2017-2030)" clearly stated that the folic acid deficiency rate of pregnant women should be controlled below 5% by 2030. The folic acid intake recommended by the World Health Organization is: 200 μg / d for adults, 60 μg / d for infants, 100 μg / d for children, and 400 μg / d for pregnant women. There are currently four main ways to increase folic acid in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/18C12R1/25
CPCC12N1/20C12P17/182C12N1/205C12R2001/25
Inventor 张健杨贞耐郝一江杨铭赵笑罗天淇朱园花张芸陈泽轩
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY