Method for extracting natural proanthocyanidins with different degrees of polymerization from cranberries
A technology of proanthocyanidins and degree of polymerization, applied in the direction of organic chemistry, which can solve the problems of increasing the risk of degradation of active substances and extremely high requirements for processes and equipment, and achieves soft extraction and purification conditions, strong operability and high yield Effect
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reference example 1
[0051] The polyamide resin used in the embodiment of the present invention is activated before use. The specific method is: after immersing the fresh resin in an ethanol solution with a volume fraction of 95% for 24 hours, rinse it with distilled water until it has no alcohol smell, and then use 2.5BV , Soak in 3.5% NaOH solution for 5.5 hours, rinse with distilled water until neutral, then soak in 2.5BV, 2.5% acetic acid solution for 5.5 hours, rinse with distilled water until neutral, and serve.
Embodiment 1
[0053] (1) Water extraction: Add 2000g of frozen cranberries with water in 3 times (the amount of water added is 20kg, 10kg, 10kg in turn), and heat extraction at 70°C respectively, and the time of each heating extraction is 3h , the time of heating, extracting and stirring each time is 5min, and then the clear liquid is obtained after passing through a decanter centrifuge;
[0054] (2) Ultrafiltration: pass the clear liquid obtained in step (1) through an ultrafiltration membrane with a molecular weight cut-off of 50,000 Da, and collect the permeate;
[0055] (3) Adsorption: Put the permeate obtained in step (2) directly on the 40-mesh polyamide resin column I for adsorption. When the leakage point is reached, start to string the column II with the same model and specification, and continue to add the permeate that is 0.8 times the weight of the loaded column;
[0056] (4) Water washing: use 1.5BV of water at a flow rate of 1.5BV / h, under the condition of series connection, ...
Embodiment 2
[0061] (1) Water extraction: dilute 400g cranberry extract concentrate with water to a dilution of 4brix;
[0062] (2) Ultrafiltration: pass the diluted solution obtained in step (1) through an ultrafiltration membrane with a molecular weight cut-off of 100,000 Da, and collect the permeate;
[0063] (3) Adsorption: Put the permeate obtained in step (2) directly on the 80-mesh polyamide resin column I for adsorption. The resin volume of column I is 200ml, the ratio of height to diameter is 5:1, and the flow rate on the column is 1.5BV / h. When the leakage point is reached, start to string the column II with the same type and specification, and continue to add permeate of 0.6 times the weight of the loaded column;
[0064] (4) Washing: use 2.5BV of water, at a flow rate of 1.0BV / h, under the condition of series connection, wash the column Ⅰ after step (3) adsorption with water, then untie the series connection, use 0.8BV of water alone, Use water at a flow rate of 1BV / h to catch...
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Abstract
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