Brevundimonas aurantiaca and application thereof to Baitiao codonopsis pilosula

A Brevimonas, yellow technology, applied in the field of microorganisms, can solve the problems of discounted effect, damage, chromosomal aberration of liver disease, etc.

Active Publication Date: 2019-09-24
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used preventive and therapeutic pesticides are carbendazim and thiophanate-methyl. Carbendazim is used for soil disinfection and seedling disinfection, but carbendazim residues can cause liver disease and chromosomal aberrations, and are toxic to mammals, while thiophanate-methyl The antibacterial effect of thiophanate in alkaline soil will be greatly reduced, so it is not suitable for the planting area of ​​Gansu Baitiao Codonopsis
The biocontrol microbial agents in the prior art are also affected by the planting environment of Codonopsis codonopsis and the antagonism of alkaloids in the roots of Codonopsis codonopsis. The occurrence of root rot
[0005] At the same time, the additional application of microorganisms other than the original soil environment will affect and damage the original soil micro-ecological environment, and the colonization of foreign strains is poor, requiring long-term application, and a large amount of application can ensure the effectiveness of microbial agents, resulting in the application of The cost of microbial agents increases, but the effect is discounted, and the ecological safety still needs to be evaluated

Method used

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  • Brevundimonas aurantiaca and application thereof to Baitiao codonopsis pilosula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation of Brevundimonas xanthomonas D3 CGMCC No.17101

[0035] Take 5g of soil sample (collected from Zhang County, Gansu Province) into a triangular flask, add 50ml of sterile water, seal it and shake it on a shaker for 20 minutes to make a suspension, absorb 5ml and add it to 100ml of nitrogen-fixing bacteria enrichment medium, 28 Cultivate at ℃, and after 72 hours, absorb 5ml of the bacterial solution and add 100ml of nitrogen-fixing bacteria enrichment medium to continue the cultivation, repeat the cultivation three times, and then isolate a single colony. Separation adopts the plate streaking method and gradient dilution method: the plate streaking method is to use the inoculation loop to dip the bacterial liquid on the solid medium of Ashubei for streaking and separation; the gradient dilution method is to divide the cultured bacterial liquid at 10 -2 、10 -4 、10 -6 、10 -8 Dilution After making a bacterial suspension with sterile water, draw 0.1ml a...

Embodiment 2

[0036] Example 2 Identification of Brevundimonas xanthomonas D3 CGMCC No.17101.

[0037] The isolated and purified D3 strain was identified from the following three aspects.

[0038] 1. Morphological identification

[0039]The D3 isolated in Example 1 was cultured as a single colony and the colonies were observed, mainly including the size, color, transparency, humidity, surface state of the colony (whether it is flat, raised, wrinkled, depressed, etc.), the state of the edge of the colony (whether neat, irregular, radial, etc.). Microscopic examination was performed after staining with biological stains.

[0040] The results showed that the colonies of strain D3 obtained after separation and purification: colony diameter 2-3 mm, colony color yellow, colony opaque, colony surface moist, slightly raised, colony edge neat. Microscopic examination was Gram-negative, with flagella and no spores.

[0041] 2. Separation of 16s rDNA sequence homology

[0042] Cultivate the bacte...

Embodiment 3

[0057] Example 3 Nitrogenase Activity Test of Brevundimonas xanthus D3 CGMCC No.17101

[0058] The Brevundimonas xanthomonas ( Brevundimonas aurantiaca ) D3 CGMCCNo.17101 was used to test the nitrogenase activity, and the acetylene reduction method was used. The specific method is as follows: prepare the suspension of the D3 strain (cells, 1×10 8 ml -1 ), take 1ml and inoculate it in a serum bottle filled with 5ml of semi-solid Ashubei culture medium, and incubate at 28°C for 48h. Replace the cap of the serum bottle with a rubber stopper, draw out 10% of the gas with a sterile syringe, then inject 10% acetylene, and incubate at 28°C for 36h. Use a sterile syringe to extract 0.2ml of the mixed gas from the serum bottle and inject it into the gas chromatograph, measure the amount of ethylene produced, and calculate the nitrogenase activity according to the following formula. N=hxCV / (24.9hst). where N is the nitrogenase activity, that is, the concentration of acetylene produc...

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Abstract

The invention provides brevundimonas aurantiaca D3 which is now stored in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms, and the registration number of the preservation center is CGMCC No.17101. D3 has high nitrogenase activity, has a significant inhibitory effect on fusarium oxysporum, and has high tolerance to extracts of Baitiao codonopsis pilosula. A D3 bacterial solution or a microbial agent with D3 as the core strain can be used as exclusive azotobacter fertilizer of the Baitiao codonopsis pilosula, and can further be used for preventing and controlling root rot of the Baitiao codonopsis pilosula caused by the fusarium oxysporum.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain of Brevundimonas chrysogenum and its application on Codonopsis pilosulae. Background technique [0002] Codonopsis Codonopsis is a traditional tonic medicine commonly used in China. It has the effects of invigorating the middle and Qi, strengthening the spleen and lungs. Modern research shows that Codonopsis pilosula contains various sugars, phenols, sterols, volatile oils, baicalein glucoside, saponins and trace alkaloids, which have the functions of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, and enhancing hematopoietic function. In addition, it can improve the leukopenia caused by chemotherapy and radiotherapy. Gansu is one of the main producing areas of Codonopsis ginseng. The annual output of Baitiao Codonopsis ginseng in Weiyuan accounts for more than 60% of the total output of Codonopsis ginseng in the country, and Weiy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01N63/02A01P3/00C12R1/01
CPCC05F11/08A01N63/00C12R2001/01C12N1/205A01N63/10
Inventor 杜文静丁品刘锦霞李晶李娜武建荣张建军
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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