Bacillus thuringiensis microcapsule with ultraviolet resistance, and preparation method thereof
A kind of Bacillus chrysogenum, anti-ultraviolet technology, applied in the direction of botanical equipment and methods, chemical instruments and methods, biocides, etc., can solve the problem of unsatisfactory performance in anti-ultraviolet
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment approach
[0047] As mentioned above, as a class of widely used biopesticides, it is its insecticidal crystal protein or its protein aggregates (parasporal crystals) that exert the toxic effect of Bt, but its application has disadvantages such as slow insecticidal speed, The residual effect period is short, and it is easily affected by environmental factors such as ultraviolet radiation, drying, and high temperature. Moreover, in the preliminary work of the laboratory, positively charged chitosan (Cs) and negatively charged sodium alginate (sodium alginate, Alg) were used as wall materials, and insecticidal protein Although the microcapsules prepared as the core have the ability to resist high temperature and drying, the performance of anti-ultraviolet is not ideal. In view of this, the present inventor has carried out a large number of researches on the preparation method, drug effect, period effect, especially anti-ultraviolet radiation stability etc. of Bacillus thuringiensis insectic...
Embodiment 1
[0091] Example 1: Preparation of Bacillus thuringiensis microcapsules with UV resistance using Cry1Ac crystals as the core 1. Preparation of microcapsule preparation reagents
[0092] 0.5M NaCl solution: 58.5g NaCl, add deionized water to make up to 2L;
[0093] 100mM Tris buffer: 121g Tris, add deionized water to make up to 1L;
[0094] 1.0 mg / mL 95% DD Cs: Dissolve 1 g 95% DD Cs in 1L 0.5M NaCl solution, stir magnetically for 6 hours, and adjust the pH to 5.0;
[0095] 1.0mg / mL Alg: Dissolve 1g Alg in 1L 0.5M NaCl solution, stir magnetically for 18h, and adjust the pH value to 5.0;
[0096] Nano-TiO 2 Complexes with Cs (TiO 2 -Cs): 2.7g TiO 2 , 2.7g Cs was dissolved in 1L deionized water, stirred magnetically for 6 hours at room temperature, and adjusted the pH value to 5.0;
[0097] Nano-ZnO suspension: take nano-ZnO particles (50 ± 10nm) and dissolve them in water with a pH of 4.0 and 7.0, stir on a magnetic stirrer for 30min, and prepare a 10g / L suspension;
[0098]...
Embodiment 2
[0113] Example 2: Investigate the ability of Cry1Ac crystal microcapsules to resist ultraviolet rays
[0114] Studies have shown that the three-dimensional structure of crystal protein is destroyed after ultraviolet irradiation, so that crystal protein cannot dissolve in alkaline solution and insect midgut environment, so it cannot play an insecticidal effect.
[0115] In this experiment, nanoparticles were used as UV protective agents, which were tightly and uniformly wrapped on the surface of microcapsules, thereby improving the ability of Cry1Ac crystal protein to resist ultraviolet radiation. After the unwrapped crystal protein and the microcapsules loaded with three different nanomaterials were air-dried in a petri dish, they were put into a solar simulator and irradiated for the same time. Take out the sample and suspend it with deionized water, keep a part for the determination of biological activity, and detect the level of insecticidal activity of insecticidal protein...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap