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Bacillus thuringiensis microcapsule with ultraviolet resistance, and preparation method thereof

A kind of Bacillus chrysogenum, anti-ultraviolet technology, applied in the direction of botanical equipment and methods, chemical instruments and methods, biocides, etc., can solve the problem of unsatisfactory performance in anti-ultraviolet

Active Publication Date: 2019-09-27
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microcapsules have the ability to resist high temperature and drying, but the performance in anti-ultraviolet is not ideal

Method used

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  • Bacillus thuringiensis microcapsule with ultraviolet resistance, and preparation method thereof
  • Bacillus thuringiensis microcapsule with ultraviolet resistance, and preparation method thereof
  • Bacillus thuringiensis microcapsule with ultraviolet resistance, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0047] As mentioned above, as a class of widely used biopesticides, it is its insecticidal crystal protein or its protein aggregates (parasporal crystals) that exert the toxic effect of Bt, but its application has disadvantages such as slow insecticidal speed, The residual effect period is short, and it is easily affected by environmental factors such as ultraviolet radiation, drying, and high temperature. Moreover, in the preliminary work of the laboratory, positively charged chitosan (Cs) and negatively charged sodium alginate (sodium alginate, Alg) were used as wall materials, and insecticidal protein Although the microcapsules prepared as the core have the ability to resist high temperature and drying, the performance of anti-ultraviolet is not ideal. In view of this, the present inventor has carried out a large number of researches on the preparation method, drug effect, period effect, especially anti-ultraviolet radiation stability etc. of Bacillus thuringiensis insectic...

Embodiment 1

[0091] Example 1: Preparation of Bacillus thuringiensis microcapsules with UV resistance using Cry1Ac crystals as the core 1. Preparation of microcapsule preparation reagents

[0092] 0.5M NaCl solution: 58.5g NaCl, add deionized water to make up to 2L;

[0093] 100mM Tris buffer: 121g Tris, add deionized water to make up to 1L;

[0094] 1.0 mg / mL 95% DD Cs: Dissolve 1 g 95% DD Cs in 1L 0.5M NaCl solution, stir magnetically for 6 hours, and adjust the pH to 5.0;

[0095] 1.0mg / mL Alg: Dissolve 1g Alg in 1L 0.5M NaCl solution, stir magnetically for 18h, and adjust the pH value to 5.0;

[0096] Nano-TiO 2 Complexes with Cs (TiO 2 -Cs): 2.7g TiO 2 , 2.7g Cs was dissolved in 1L deionized water, stirred magnetically for 6 hours at room temperature, and adjusted the pH value to 5.0;

[0097] Nano-ZnO suspension: take nano-ZnO particles (50 ± 10nm) and dissolve them in water with a pH of 4.0 and 7.0, stir on a magnetic stirrer for 30min, and prepare a 10g / L suspension;

[0098]...

Embodiment 2

[0113] Example 2: Investigate the ability of Cry1Ac crystal microcapsules to resist ultraviolet rays

[0114] Studies have shown that the three-dimensional structure of crystal protein is destroyed after ultraviolet irradiation, so that crystal protein cannot dissolve in alkaline solution and insect midgut environment, so it cannot play an insecticidal effect.

[0115] In this experiment, nanoparticles were used as UV protective agents, which were tightly and uniformly wrapped on the surface of microcapsules, thereby improving the ability of Cry1Ac crystal protein to resist ultraviolet radiation. After the unwrapped crystal protein and the microcapsules loaded with three different nanomaterials were air-dried in a petri dish, they were put into a solar simulator and irradiated for the same time. Take out the sample and suspend it with deionized water, keep a part for the determination of biological activity, and detect the level of insecticidal activity of insecticidal protein...

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Abstract

The invention relates to a bacillus thuringiensis microcapsule with ultraviolet resistance, and a preparation method thereof. According to the bacillus thuringiensis microcapsule with ultraviolet resistance, bacillus thuringiensis crystal protein (for example, Cry1Ac crystal) is taken as a core, two kinds of polyelectrolytes oppositely charged under preparation conditions are selected, layer-by-layer self-assembling is adopted to form a capsule wall on the surface of the bacillus thuringiensis crystal protein, and the external surface of the capsule wall is loaded with an anti-ultraviolet nanometer material. The bacillus thuringiensis microcapsule possesses high insecticidal effect, long service life, and excellent high temperature resistance, dryness resistance, and anti-ultraviolet stability.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological preparations, and relates to a Bacillus thuringiensis microcapsule with anti-ultraviolet ability and a preparation method thereof. Background technique [0002] Bacillus thuringiensis (Bt) is a ubiquitous Gram-positive aerobic bacterium, which belongs to the second group of group 18 Bacillus in the ninth edition of "Bergey's Bacterial Identification Manual" kind. The difference from other closely related bacillus is that Bt can also produce insecticidal crystal proteins in shapes such as rhombus, bicone, ellipsoid, rectangle and irregular shapes, namely parasporal crystals, while producing spores. [0003] Parasporal crystals are composed of one or more Cytolitic (Cyt) toxin proteins and Crystal (Cry) toxin proteins. Each parasporal crystal is only specific for a narrow-spectrum insect. The main insecticidal spectrum includes Lepidoptera, Homoptera, Orthoptera, Hymenoptera, Isopt...

Claims

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Application Information

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IPC IPC(8): A01N25/28A01N63/00A01N37/46A01P7/04C07K14/325
CPCA01N25/28A01N37/46A01N63/00C07K14/325
Inventor 郭淑元张永静张爱晶
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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