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A kind of preparation method and application of modified hickory chick polysaccharide

A technology of Morchella and polysaccharide, applied in directions such as medical preparations, pharmaceutical formulations, organic active ingredients containing active ingredients, etc., can solve problems such as weak alpha-amylase inhibitory ability, and achieve a high alpha-amylase inhibitory rate. Effect

Active Publication Date: 2021-06-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the commonly used hypoglycemic drug acarbose, its inhibitory ability to α-glucosidase, sucrase and maltase is not weaker than that of acarbose, but its inhibitory ability to α-amylase is much weaker acarbose

Method used

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  • A kind of preparation method and application of modified hickory chick polysaccharide
  • A kind of preparation method and application of modified hickory chick polysaccharide
  • A kind of preparation method and application of modified hickory chick polysaccharide

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Experimental program
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Effect test

Embodiment 1

[0024] Extraction of polysaccharides from Morchella: take the body of Morchella, wash and remove impurities, put it in an oven at 105°C for sterilization for 10 minutes, and dry it at 65°C. The dried hickory chick body was crushed and sieved through a 40-mesh sieve, mixed with distilled water at a ratio of 1:10, and extracted by ultrasonic heating at 55°C and 400W power for 45min to obtain an extract. The extract was allowed to stand at 4°C for 12 hours, filtered through 4 layers of gauze, and centrifuged to obtain a supernatant, which was concentrated in a water bath at 85°C, then added with 6 times the amount of absolute ethanol, and stood at -20°C for 12 hours. The precipitate was collected and vacuum freeze-dried to obtain the morel polysaccharide.

[0025] Modification of morel polysaccharide: Weigh 1g of the above-mentioned morel polysaccharide, add 5mL of 30% hydrogen peroxide aqueous solution to dissolve the sample according to the ratio of solid to liquid 1:5, and the...

Embodiment 2

[0027] The extraction of hickory chick polysaccharide is the same as embodiment 1.

[0028] Modification of morel polysaccharide: Weigh 1g of the above-mentioned morel polysaccharide, add 5mL of 30% hydrogen peroxide aqueous solution to dissolve the sample according to the ratio of solid to liquid 1:5, and then add 0.2mol / L disodium hydrogen phosphate with pH=7 - Sodium dihydrogen phosphate buffer solution was adjusted to 25 mL, and reacted at a temperature of 40°C for 3 hours. After the reaction, it was concentrated and vacuum freeze-dried to obtain a modified product, that is, a modified Morchella polysaccharide.

Embodiment 3

[0030] The extraction of hickory chick polysaccharide is the same as embodiment 1.

[0031] Modification of morel polysaccharide: Weigh 1g of the above-mentioned morel polysaccharide, add 3mL of 30% hydrogen peroxide aqueous solution to dissolve the sample according to the ratio of solid to liquid 1:3, and then add 0.2mol / L disodium hydrogen phosphate with pH=7 - Sodium dihydrogen phosphate buffer solution was adjusted to 25 mL, and reacted for 3 hours at a temperature of 40 ° C. After the reaction was completed, it was concentrated and vacuum freeze-dried to obtain a modified product, namely modified Morchella polysaccharide.

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Abstract

The invention discloses a preparation method of modified hickory chick polysaccharide, comprising the following steps: (1) washing hickory chick, removing impurities, high-temperature sterilization, drying, crushing and sieving, and carrying out water extraction and alcohol precipitation Extract morel polysaccharides; (2) add morel polysaccharides to hydrogen peroxide aqueous solution, then add disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution to react, concentrate after the reaction, and freeze-dry to obtain modified morel bacterial polysaccharides. The present invention uses the hydrogen peroxide oxidation method to modify the hickory chick polysaccharide, and the modified hickory chick polysaccharide significantly improves the inhibition rate of α-amylase; meanwhile, the inhibition rate of sucrase, the inhibition rate of maltase, and the inhibition rate of α-glucoside Enzyme also has corresponding improvement; Compared with the acarbose under the same concentration, modified hickory chick polysaccharide of the present invention has no obvious difference except sucrase inhibitory rate and acarbose, to the inhibition of other three kinds of glycosidases. rate increased accordingly.

Description

technical field [0001] The invention relates to a preparation method and application of morel polysaccharide, in particular to a preparation method and application of modified morel polysaccharide. Background technique [0002] Glycosidase is an enzyme that can hydrolyze glycosidic bonds. It plays a key role in the hydrolysis and synthesis of sugar and sugar complexes. It catalyzes the decomposition of sucrose and maltose in the small intestine, and the decomposition product glucose enters the blood, which is causing postprandial hyperglycemia. one of the main factors. Lowering blood sugar by delaying the digestion and absorption of carbohydrates is an important way to treat diabetes. Therefore, taking glycosidase inhibitors can inhibit the digestion of carbohydrates, slow down the absorption of carbohydrates in the human body, and finally achieve the purpose of inhibiting the rapid rise of blood sugar after meals. Among the drugs for the treatment of type 2 diabetes, glyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10
CPCA61K31/715A61P3/10C08B37/0003C08B37/006
Inventor 张雪松谢春芹凡军民曹正许俊齐刘焕颖苗雪
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY