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Salt-resistant denitrifying bacterium and application thereof

A denitrifying bacteria and salt-tolerant technology, applied in the field of environmental microorganisms, can solve problems such as the inability to remove nitrite nitrogen, and achieve good practical value and application prospects

Active Publication Date: 2019-09-27
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention aims to solve the technical problem that the nitrogen removal ability of the existing common denitrifying bacteria is inhibited by high salinity and cannot remove nitrite nitrogen in the saline aquaculture wastewater, and provides a salt-tolerant denitrifying bacteria and its application

Method used

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  • Salt-resistant denitrifying bacterium and application thereof
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  • Salt-resistant denitrifying bacterium and application thereof

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specific Embodiment approach 1

[0025] Specific embodiment 1: In this embodiment, a salt-tolerant denitrifying bacterium, which is Alishewanella sp.F2, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District. The preservation date was March 25, 2019, in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the number is CGMCC No: 17433.

[0026] The Alishewanella sp.F2 colony in this embodiment is white and round, with a diameter of 1-2 mm, neat edges, smooth surface, sticky and shiny. Observed under a microscope, the bacteria are rod-shaped, with a length of 1-2 μm and a width of 0.3-0.5 μm.

[0027] The physiological and biochemical characteristics of Alishewanella sp.F2 in this embodiment are as shown in Table 1:

[0028] Table 1 Physiological and biochemical characteristics of Alishewanella sp.F2

...

specific Embodiment approach 2

[0031] Specific embodiment two: the screening method of Alishewanella sp.F2 in this embodiment is realized according to the following steps:

[0032] Step 1: Take 2mL of muddy water samples collected from the seawall of Zhuanghe City, Dalian City, Liaoning Province (39°38′31″N latitude 122°58′19″East longitude) and put it into a 250mL conical flask, add 200mL strains for denitrification culture The bottle mouth is sealed with a sealing film to create an anoxic environment; the enriched culture solution is obtained after standing at a constant temperature for 5 to 7 days at a temperature of 30°C;

[0033]Step 2: Gradiently dilute the enriched culture solution in Step 1 using the doubling dilution method, pipette 0.5mL of each dilution suspension into the strain screening plate culture medium (which has been placed overnight, no bacteria growth), and coat with a glass triangle. After spreading the enriched culture solution evenly with a cloth stick, pour the second layer of ster...

specific Embodiment approach 3

[0052] Specific embodiment three: In this embodiment, the determination of the salt tolerance of Alishewanella sp.F2 is as follows:

[0053] Prepare screening and separation media with different salinity gradients, that is, the NaCl concentrations are 0g / L, 30g / L, 50g / L, 70g / L, and 100g / L; inoculate strain F2 onto the medium plates of various salinities Put it into a constant temperature incubator at 30°C and cultivate it for 5 days. Whether the strain grows is used as the basis for whether the strain can withstand the corresponding salinity; the culture medium not inoculated with denitrifying bacteria is used as the blank control, and each group has 3 parallels.

[0054] After 1 day of culture, no colonies appeared in the medium of each salinity; after 2 days of culture, there were obvious colonies on the medium containing 0g / L and 30g / L NaCl, containing 50g / L, 70g / L and 100g / L There was a small amount of colony growth on the medium containing L NaCl; after 5 days of cultivat...

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Abstract

The invention discloses a salt-resistant denitrifying bacterium and application thereof, relates to the technical field of environment microorganisms and aims to solve the technical problems that the denitrogenation capability of a conventional common denitrifying bacterium is limited by a high salt degree and nitrite nitrogen in salt-containing breeding wastewater cannot be removed. The bacterium is Alishewanella sp.F2, is preserved in the China General Microbiological Culture Collection Center, the preservation date is March 25, 2019, and the preservation number is CGMCC No: 17433. The bacterium is capable of implementing denitrogenation and denitrification under a salt-containing condition. The bacterium disclosed by the invention is adopted to remove nitrite nitrogen pollutants in a water body.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms. Background technique [0002] With the rapid development of marine aquaculture, most of them have entered the form of semi-intensive or intensive aquaculture. Many factors such as high stocking density and excessive feeding residual bait have caused the increase of nitrite nitrogen in the aquaculture water year by year. Nitrite nitrogen is not only one of the eutrophication factors in water bodies, but also an intermediate product of nitrification and denitrification, so it widely exists in natural water bodies and is likely to cause great harm to aquatic animals and human health. Nitrite in water is highly toxic. Humans drinking high-concentration nitrite water for a long time will lead to mental retardation and even death; aquatic animals are more sensitive to nitrite toxicity due to the accumulation of nitrite, resulting in insufficient oxygen content in aquatic animal tis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F103/20
CPCC02F3/34C02F2101/166C02F2103/20C12N1/205C12R2001/01
Inventor 祝惠王鑫壹阎百兴于翔霏陈欣
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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