Nucleic acid composition, detection kit for influenza virus and micro-fluidic chip

A nucleic acid composition and microfluidic chip technology, applied in the biological field, can solve the problems of long period of virus isolation and culture and immunological detection, low PCR throughput and low detection sensitivity, etc.
CN110283940APending Publication Date: 2019-09-27深圳市呈晖医疗科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
深圳市呈晖医疗科技有限公司
Publication Date
2019-09-27

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Abstract

The invention relates to nucleic acid composition, a detection kit for an influenza virus and a micro-fluidic chip. The nucleic acid composition comprises detection primer pairs, wherein the detection primer pairs comprise at least two of the following primer pairs: an influenza A primer pair, an influenza B primer pair, an influenza C primer pair, an influenza A H1 primer pair, an influenza A H3 primer pair, an influenza A H5 primer pair, an influenza A H7 primer pair, an influenza A H9 primer pair, an influenza A N9 primer pair and a high-pathogenicity influenza A H7N9 primer pair. According to the nucleic acid composition, interference between the primers and probes can be avoided, at least two influenza viruses are detected simultaneously once, and detection sensitivity can reach 100 copies / mL.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a nucleic acid composition, an influenza virus detection kit and a microfluidic chip. Background technique

[0002] In terms of virological classification, influenza virus (Influenza virus) belongs to Orthomyxoviridae (Orthomyxoviridae), and influenza virus includes influenza A virus, influenza B virus, influenza C virus and influenza D virus. Influenza A virus, influenza B virus, and influenza C virus can infect humans. Among them, influenza A virus has a wide host range, numerous serotypes, and frequent cross-species transmission. It is an important zoonotic pathogen and the main pathogen that causes new influenza pandemics.

[0003] Influenza virus is an enveloped virus whose genome contains eight single-stranded negative-sense RNA segments. Enveloped viruses have a host-derived lipid bilayer containing two major surface viral glycoproteins responsible for viral attachment: hema...

Claims

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