Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nucleic acid composition, detection kit for influenza virus and micro-fluidic chip

A nucleic acid composition and microfluidic chip technology, applied in the biological field, can solve the problems of long period of virus isolation and culture and immunological detection, low PCR throughput and low detection sensitivity, etc.

Pending Publication Date: 2019-09-27
深圳市呈晖医疗科技有限公司
View PDF8 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection cycle of virus isolation and culture and immunology is long, and the detection sensitivity is low, which cannot meet the actual needs
However, the throughput of PCR is low, and only one pathogen can be detected at a time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid composition, detection kit for influenza virus and micro-fluidic chip
  • Nucleic acid composition, detection kit for influenza virus and micro-fluidic chip
  • Nucleic acid composition, detection kit for influenza virus and micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0096] The preparation method of the above-mentioned microfluidic chip includes the step of preparing the PCR reaction reagent, the internal standard and the above-mentioned nucleic acid composition into freeze-dried powder.

[0097] Specifically, the PCR reaction reagents, the internal standard and the above nucleic acid composition were respectively frozen and stored at -80°C for 8h-10h. Then carry out vacuum freeze-drying. Concrete vacuum freeze-drying process parameter is as follows table 1:

[0098] Table 1

[0099]

[0100]The PCR reaction reagent, the internal standard and the above nucleic acid composition after freeze-drying according to the above process can be stored and transported at room temperature, and the validity period of the reagent can be extended.

[0101] The above method of using the microfluidic chip includes step S110 and step S130. Specifically:

[0102] Step S110, extracting nucleic acid.

[0103] Specifically, the sample is added to the sam...

Embodiment 1

[0111] The structure of the microfluidic unit of the microfluidic chip of Example 1 is as follows figure 1 shown. The microfluidic unit includes a sampling pool, a buffer pool, a lysate storage pool, a lysis pool, a purification pool, a reaction pool, a waste liquid pool, and a liquid storage pool. A control valve is provided between the liquid storage pool and the waste liquid pool. The control valve is Paraffin valve. In the microfluidic unit:

[0112] An internal standard is set in the buffer pool, and the internal standard is freeze-dried powder. The internal standard consists of an internal standard primer pair and an internal standard probe, and the specific sequences of the internal standard primer pair and internal standard probe are shown in Table 2;

[0113] Lysis solution is accommodated in the lysate storage tank, and the lysate is composed of guanidine hydrochloride with a final concentration of 5mol / L, C with a final volume percentage of 40%. 2 h 5 OH, Tris-...

Embodiment 2

[0122] (1) Obtain 20 clinical samples and number them from 1 to 20, including 10 influenza virus positive samples, 1 negative sample and 9 other subtype influenza A virus samples or virus samples that can cause influenza-like symptoms in humans. specifically:

[0123] Sample No. 1 is positive for influenza A (H10N2), sample No. 2 is positive for influenza B, sample No. 3 is positive for influenza C, sample No. 4 is positive for influenza A (H1N1), and sample No. 5 is positive for influenza A (H1N1). Influenza A H3N1 virus positive sample, sample No. 6 is a positive sample of influenza A H5N1 virus, sample No. 7 is a positive sample of influenza A H7N1 virus, sample No. 8 is a positive sample of highly pathogenic influenza A H7N9 virus, sample No. 9 Sample No. 10 is a positive sample of influenza A H9N1 virus, sample No. 10 is a positive sample of highly pathogenic influenza A H7N9 virus, sample No. 11 is a negative sample of influenza virus, sample No. 12 is a positive sample ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to nucleic acid composition, a detection kit for an influenza virus and a micro-fluidic chip. The nucleic acid composition comprises detection primer pairs, wherein the detection primer pairs comprise at least two of the following primer pairs: an influenza A primer pair, an influenza B primer pair, an influenza C primer pair, an influenza A H1 primer pair, an influenza A H3 primer pair, an influenza A H5 primer pair, an influenza A H7 primer pair, an influenza A H9 primer pair, an influenza A N9 primer pair and a high-pathogenicity influenza A H7N9 primer pair. According to the nucleic acid composition, interference between the primers and probes can be avoided, at least two influenza viruses are detected simultaneously once, and detection sensitivity can reach 100 copies / mL.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid composition, an influenza virus detection kit and a microfluidic chip. Background technique [0002] In terms of virological classification, influenza virus (Influenza virus) belongs to Orthomyxoviridae (Orthomyxoviridae), and influenza virus includes influenza A virus, influenza B virus, influenza C virus and influenza D virus. Influenza A virus, influenza B virus, and influenza C virus can infect humans. Among them, influenza A virus has a wide host range, numerous serotypes, and frequent cross-species transmission. It is an important zoonotic pathogen and the main pathogen that causes new influenza pandemics. [0003] Influenza virus is an enveloped virus whose genome contains eight single-stranded negative-sense RNA segments. Enveloped viruses have a host-derived lipid bilayer containing two major surface viral glycoproteins responsible for viral attachment: hema...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12M1/00C12M1/34
CPCC12Q1/701C12Q1/686C12Q2563/107C12Q2565/629Y02A50/30
Inventor 游腾飞
Owner 深圳市呈晖医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com