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Solid-state quantum dot sensor as well as preparation method and application thereof

A technology of quantum dots and sensors, applied in the field of fluorescence detection, can solve the problem that quantum dots need to be further improved, and achieve the effect of high sensitivity and simplified detection process

Active Publication Date: 2019-10-01
XIAN JIAOTONG LIVERPOOL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing technology, the stability, detection sensitivity and detection response speed of quantum dots in the sensor need to be further improved.

Method used

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  • Solid-state quantum dot sensor as well as preparation method and application thereof
  • Solid-state quantum dot sensor as well as preparation method and application thereof
  • Solid-state quantum dot sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1 is used to detect the solid-state quantum dot sensor preparation method of hydrogen peroxide

[0082] 0.5 ml of polyvinyl alcohol (PVA) (1%) and 0.5 ml of perfluorosulfonic acid (nafion) (0.05%) were mixed in a 10 ml bottle and stirred at room temperature for 15 minutes. The mixing ratio of PVA-nafion is 20:1. Add 30 μL of QD (1.78 mg / mL) to 1 mL of the prepared PVA-nafion solution and keep stirring for 5 minutes. The mixture was sonicated for 30 seconds. A homogeneous solution of nafion-PVA and QDs was obtained after mixing and sonication, which was immediately used to prepare sensors. Sensors were fabricated on 96-well plates. Incorporate 20 μL of PVA-nafion-QDs membranes into 96-well plates or PET sheets and dry in the open air at room temperature overnight.

[0083] Wherein, the structural formulas of perfluorosulfonic acid and polyvinyl alcohol are shown in formulas I and II respectively, and the structural formula of quantum dot CdTe modified with...

Embodiment 2

[0086] Embodiment 2 is used to detect the preparation method of the solid-state quantum dot sensor of glucose

[0087] Enzyme-modified sensors were prepared by adding 40 μL of QDs solution (1.87 mg / mL) to 0.5 mL of a solution containing 1.0% nafion and 0.5% PVA. Glucose oxidase stock solution was prepared by dissolving 2 mg of solid GOx (glucose oxidase) in 1.5 mL of water. Add 50 µL of GOx stock solution to the mixture of QDs and polymer. 25 μL of the finally obtained solution was added to each microglass cuvette of a commercial 96-well plate, and then dried at room temperature for 3 h. The fabricated sensors were stored in a refrigerator at 4 °C before analysis. The preparation process of the enzyme-modified solid-state quantum dot sensor is shown in figure 1 .

[0088] Calibration of Quantum Dots in Hydrogen Peroxide Solution

[0089] Briefly, 25 mL of a 0.238 g / L solution of QDs (in 5 mM potassium phosphate buffer) was placed in an Erlenmeyer flask and the solution wa...

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Abstract

The invention relates to a solid-state quantum dot sensor as well as a preparation method and application thereof. Quantum dots are embedded into a polymer matrix formed by perfluorosulfonic acid andpolyvinyl alcohol to form the solid-state quantum dot sensor. Glucose oxidase is embedded into the solid-state quantum dot sensor to generate a solid-state quantum dot glucose sensor. According to thesolid-state quantum dot sensor provided by the invention, the quantum dots and the glucose oxidase are stable in the sensor by specifically selecting a polymer matrix, when the sensor is exposed on atest sample, rapid response can be realized, and the detection efficiency and the sensitivity are improved.

Description

technical field [0001] The invention relates to the field of fluorescence detection, in particular to a solid-state quantum dot sensor for detecting hydrogen peroxide or glucose and a preparation method thereof. Background technique [0002] Quantum Dots (QD) is a kind of nanoparticle, mainly composed of II-VI, III-V or IV. The characteristic size of the material can be compared with the de Broglie wavelength or Compared with the mean free path of electrons, the energy of electrons is quantized in three dimensions. Quantum dots have unique optical properties, such as broad absorption peaks, narrow emission peaks, good photostability, and fluorescence intensity, which make quantum dots widely used in the fields of physics, chemistry, electronics, and biology. Studies have shown that quantum dots can be used to prepare fiber-optic glucose biosensors, which are more sensitive than traditional methods for detecting glucose. However, in the prior art, the stability, detection s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/02C09K11/88
CPCG01N21/6428G01N21/64C09K11/025C09K11/883G01N2021/6417G01N21/648G01N33/588C12Q1/006C12Q1/26
Inventor 刘景堂埃麽提阿斯·啊嗎特
Owner XIAN JIAOTONG LIVERPOOL UNIV
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