A kind of method of promoting spore production of pathogenic bacteria of sorrel blight

A technology for pathogenic bacteria and blight, applied in the field of microorganisms, can solve the problems of few conidia, restrict the application of blight pathogenic bacteria, difficult to complete pathogenicity determination and drug screening test, etc., and achieve the promotion of vegetative growth and reproductive growth. Effect

Active Publication Date: 2021-11-09
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many mediums for cultivating fungi, such as PDA medium, oat medium, corn flour medium, carrot medium, etc., but these mediums cannot meet the requirements of rapid growth of the pathogenic bacteria of sweet potato blight, which has become The bottleneck in the research of this bacterium makes it difficult to complete the pathogenicity assay and drug screening test of this bacterium
In addition, it is difficult to induce the conidia of the fungus Blight blight. Although some specific media can meet the requirements of rapid growth of mycelia, there are few conidia produced, which seriously restricts the pathogenic bacteria Blight blight. Application in related research of sweet potato breeding and pathology

Method used

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  • A kind of method of promoting spore production of pathogenic bacteria of sorrel blight
  • A kind of method of promoting spore production of pathogenic bacteria of sorrel blight
  • A kind of method of promoting spore production of pathogenic bacteria of sorrel blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the influence of the culture medium of different carbon source, nitrogen source on the growth of sweet potato blight pathogenic bacteria

[0033] In this example, on the basis of Chapei's medium, the effects of different carbon and nitrogen sources on the pathogen of sweet potato blight were studied by changing the carbon and nitrogen sources.

[0034] The components of Cha's medium are: 1 g of dipotassium hydrogen phosphate, 0.5 g of potassium chloride, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 O) 0.5g, ferrous sulfate is 0.01g, potassium nitrate 3g, sucrose 30g, agar is 20g.

[0035] Maltose, xylose, fructose, lactose, mannose, soluble starch, dextrin, sorbitol, glucose, galactose, trehalose, ribose, cellulose, and rhamnose were used to replace sucrose in Chapei's medium, keeping The nitrogen source in each culture medium was potassium nitrate, and 14 kinds of culture media with different carbon sources were prepared.

[0036] L-cysteine, glycine,...

Embodiment 2

[0055] Embodiment 2: the influence of different mediums on the growth of sweet potato blight pathogenic bacteria

[0056] (1) The preparation of Cha's medium with maltose as the sugar source and peptone as the nitrogen source and the cultivation of the pathogenic bacteria of the blight

[0057] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (0) 0.5g, ferrous sulfate is 0.01g, peptone 3g, maltose 30g, agar are 20g;

[0058] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL, and adjust to pH=7;

[0059] Step 3: Place the prepared medium in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0060] Step 4: Pour the sterilized culture medium into the plate in a sterile environment for standby;

[0061] Step 5: In a sterile environment, use sterilized tweezers to pick up the sweet potato blight pathogenic bacteria cake with...

Embodiment 3

[0093] Embodiment 3: the impact of culture conditions on the spore production of sweet potato blight pathogenic bacteria

[0094] According to the experimental results of Example 2, it is found that dark culture can not induce sporulation of the pathogenic bacterium of the blight, although the growth rate of the pathogen can be improved through the optimization of the medium, it cannot effectively promote the sporulation. Therefore, the present invention further improves the sporulation of the blight The culture conditions of the pathogenic bacteria were optimized in order to obtain a method that can promote the spore production of the pathogenic bacteria of Blight blight.

[0095] In this example, several different culture media were taken as examples to study the mycelium growth and spore production under different culture conditions.

[0096] A: The culture medium with optimized carbon source and nitrogen source, the medium components are: dipotassium hydrogen phosphate 1...

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Abstract

The invention discloses a method for promoting the spore production of the pathogenic bacteria of vine blight. The method comprises: inoculating the pathogenic bacterium of vine blight on the culture medium, and cultivating alternately light and dark in a constant temperature incubator to obtain spores, wherein the Light-dark alternate culture is 12h light-12h dark alternate culture, wherein the light source of light is an ultraviolet lamp, and the wavelength of the ultraviolet lamp is 280nm. By optimizing the light-dark culture time, light source and light source wavelength, the present invention makes the mycelium transition from the vegetative growth stage to the reproductive growth stage on the premise of not affecting the vegetative growth of the pathogenic fungus mycelia, and promotes the growth of the pathogenic fungus of the fungal blight. The formation of conidia can obtain a large number of spores within 7 days, which can fully meet the needs of the number of conidia of the pathogenic fungus of Blight blight in the actual research work of pathology and breeding.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a culture medium for cultivating the pathogenic bacteria of variegated blight and a cultivation method for promoting the spore production of the pathogenic bacterium of variegated blight. Background technique [0002] Sweet potato (Dioscorea esculenta (Lour.) Burkill) named sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc., is a twining herbaceous vine of Convolvulaceae Dioscorea. Native to tropical America, it was introduced into China at the end of the 16th century, and there are currently more than 500 species. Sweet potatoes are distributed from south to north and from east to west in China, and are widely planted in Sichuan, Henan, Shandong, Guangzhou, Anhui, Zhejiang and other places. my country's sweet potato planting area accounts for about 70% of the world's total. More than 100 million tons, accounting for 80% of the world's total output. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N13/00C12N3/00C12R1/645
CPCC12N1/14C12N3/00C12N13/00
Inventor 谢昀烨王汉荣方丽王连平王会福余山红
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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