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Preparation method of whey protein and pectin composite double-emulsion simultaneously embedding arbutin and coumaric acid and product thereof

A technology of whey protein and arbutin, which is applied in the direction of medical preparations, cosmetic preparations, toiletry preparations, etc. Desorption and other issues, to achieve improved bioavailability, significant application, and enhanced whitening effect

Inactive Publication Date: 2019-10-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional double emulsions only use surfactants to maintain the stability between different phase interfaces. Because surfactants are easy to desorb and have replacement phenomena, traditional double emulsions are thermodynamically unstable, and they are prone to delamination during processing and storage. Flocculation and other phenomena

Method used

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  • Preparation method of whey protein and pectin composite double-emulsion simultaneously embedding arbutin and coumaric acid and product thereof
  • Preparation method of whey protein and pectin composite double-emulsion simultaneously embedding arbutin and coumaric acid and product thereof
  • Preparation method of whey protein and pectin composite double-emulsion simultaneously embedding arbutin and coumaric acid and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Dissolve arbutin, gelatin and sodium chloride in water, stir magnetically until fully dissolved at 1000rpm, obtain an aqueous solution containing 500 μg / mL arbutin, 30 μg / mL gelatin and 30 μg / mL sodium chloride, as Inner water phase W1;

[0056] (2) Dissolve coumaric acid and PGPR in olive oil, stir magnetically at 1000 rpm until fully dissolved, and obtain an oil solution containing 500 μg / mL coumaric acid and 80 μg / mL PGPR as oil phase O1;

[0057] (3) Add 10mL of internal water phase W1 dropwise to 30mL of oil phase O1, and place it under a probe-type ultrasonic instrument. After completion, the W1 / O1 emulsion was obtained, and stood in a refrigerator at 4°C for 30 minutes;

[0058] (4) Dissolve whey protein and pectin in water, mix and stir magnetically at 1000 rpm for 10 minutes, adjust the pH value to 3.5, and obtain 30 μg / mL whey protein and 10 μg / mL pectin as the external water phase W2;

[0059] (5) Take 10mL W1 / O1 emulsion and drop it into 30mL whey prot...

Embodiment 2

[0065] (1) Dissolve arbutin, gelatin and sodium chloride in water, stir magnetically until fully dissolved at 1000rpm, obtain an aqueous solution containing 500 μg / mL arbutin, 30 μg / mL gelatin and 30 μg / mL sodium chloride, as Inner water phase W1;

[0066] (2) Dissolve coumaric acid and PGPR in olive oil, stir magnetically at 1000 rpm until fully dissolved, and obtain an oil solution containing 500 μg / mL coumaric acid and 80 μg / mL PGPR as oil phase O1;

[0067] (3) Add 10mL of internal water phase W1 dropwise to 30mL of oil phase O1, and place it under a probe-type ultrasonic instrument. After completion, the W1 / O1 emulsion was obtained, and stood in a refrigerator at 4°C for 30 minutes;

[0068] (4) Dissolve whey protein and pectin in water, mix and stir magnetically at 1000 rpm for 10 minutes, adjust the pH value to 3.5, and obtain 20 μg / mL whey protein and 20 μg / mL pectin as the external water phase W2;

[0069] (5) Take 10mL W1 / O1 emulsion and drop it into 30mL whey prot...

Embodiment 3

[0073] (1) Dissolve arbutin, gelatin and sodium chloride in water, stir magnetically until fully dissolved at 1000rpm, obtain an aqueous solution containing 500 μg / mL arbutin, 30 μg / mL gelatin and 30 μg / mL sodium chloride, as Inner water phase W1;

[0074] (2) Dissolve coumaric acid and PGPR in olive oil, stir magnetically at 1000 rpm until fully dissolved, and obtain an oil solution containing 500 μg / mL coumaric acid and 80 μg / mL PGPR as oil phase O1;

[0075] (3) Add 10mL of internal water phase W1 dropwise to 30mL of oil phase O1, and place it under a probe-type ultrasonic instrument. After completion, the W1 / O1 emulsion was obtained, and stood in a refrigerator at 4°C for 30 minutes;

[0076] (4) Dissolve whey protein and pectin in water, mix and stir magnetically at 1000 rpm for 10 minutes, adjust the pH value to 3.5, and obtain 10 μg / mL whey protein and 30 μg / mL pectin as the external water phase W2;

[0077] (5) Take 10mL W1 / O1 emulsion and drop it into 30mL whey prot...

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Abstract

The invention discloses a whey protein and pectin composite double-emulsion simultaneously embedding arbutin and coumaric acid and a preparation method thereof. The preparation method comprises the steps that (1) the hydrophilic arbutin, gelatin and sodium chloride are dissolved in water, the hydrophobic coumaric acid and a W / O type emulsifier are dissolved in oil, an aqueous solution is dropwiseadded into the oil solution, and ultrasonic treatment is carried out to obtain an emulsion; (2) whey protein and pectin are dissolved in water, the pH value is adjusted, and an evenly mixed whey protein and pectin compound solution is obtained; (3) the emulsion is dropwise added into the whey protein and pectin compound solution, ultrasonic treatment is conducted, and the double-emulsion is obtained. The process is simple, the obtained double-emulsion can simultaneously embed the arbutin and the coumaric acid, the sustained-release performance is good, the double-emulsion is stable in storageand is applied to food and cosmetics as an additive, the biological activity function of products is enhanced, and therefore, the double-emulsion has wide application prospects in the fields of food and cosmetics.

Description

technical field [0001] The invention relates to the technical field of emulsion preparation, in particular to a whey protein pectin composite double emulsion simultaneously embedding arbutin and coumaric acid and a preparation method thereof. Background technique [0002] Both arbutin and coumaric acid are important plant phenolic compounds, which have been widely used in food, cosmetics and medicines. Among them, arbutin has skin whitening, anti-aging and anti-cancer effects; coumaric acid has anti-oxidation, Whitening, anti-inflammatory and inhibiting the activity of cancer cells. Due to the hydrophilicity of arbutin and the hydrophobicity of coumaric acid, the simultaneous application of these two substances has certain difficulties. In addition, studies have shown that arbutin and coumaric acid cannot exist stably in certain environments, such as high acidity, high temperature, enzymes and ultraviolet radiation, which also limit the wide application of arbutin and couma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K8/365A61K8/64A61K8/73A61K8/92A61K8/65A61K8/06A61Q19/02A23L33/00
CPCA23V2002/00A61K8/066A61K8/365A61K8/602A61K8/64A61K8/65A61K8/73A61K8/922A61K2800/82A61Q19/02A23L33/00A23V2200/318
Inventor 罗自生黄皓李莉徐艳群
Owner ZHEJIANG UNIV
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